Addgene

We narrowed to 21,140 results for: tes

Showing: 301 - 320 of 21140 results

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p3E-TurboID

Plasmid

#166570

Purpose

Multisite Gateway 3' entry clone for fusions onto the N-terminus of TurboID. Parton lab clone JSS

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

TurboID

Use

Multisite gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pSB1C3-Lux

Plasmid

#109383

Purpose

Arabinose inducible expression of the Vibrio Fischery Lux operon. Used to test burden response in E. coli

Depositor

Tom Ellis

Article

Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

Insert

pARABAD-LUX operon

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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mKate2-P2A-APEX2-csGBP

Plasmid

#108875

Purpose

Expression of APEX2 fused to a conditionally stable nanobody against GFP (includes red cytoplasmic reporter)

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

mKate2-P2A-APEX2-csGBP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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LV-TRE-VP64 mouse MyoD-T2A-dsRedExpress2

Plasmid

#60625

Purpose

Expresses VP64 mouse MyoD and dsRedExpress2 in response to doxycycline

Depositor

Charles Gersbach

Article

Kabadi et al ACS Synth Biol. 2014 Dec 10.

Insert

VP64 mouse MyoD

Use

Lentiviral

Tags

Expression

Mutation

Promoter

TRE

Available since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pME-mKate2-NS

Plasmid

#109729

Purpose

Multisite gateway vector for 5' tagging with mKate2

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

mKate2

Use

Zebrafish plasmids

Tags

Expression

Mutation

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pLys-M1

Plasmid

#109382

Purpose

Arabinose inducible VioB-mCherry expression (stronger RBS). Used to test burden response in E. coli

Depositor

Tom Ellis

Article

Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

Insert

pARABAD-VioB-mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 29, 2018

Availability

Academic Institutions and Nonprofits only

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pLV-CMV-tagRFP-Mad2

Plasmid

#114021

Purpose

Lentiviral delivery of tagRFP-MAD2 expressed from the CMV promoter

Depositor

Geert Kops

Article

Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

Insert

tagRFP-Mad2 (MAD2L1) (MAD2L1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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1457_pDEST_miniTol2_R4-R2_Cryst_mCherry

Plasmid

#171796

Purpose

pDEST miniTol2-clone for R4-R2 3-component gateway assembly (p5E + pME). Include a mCherry selection marker (Crystallin promoter Lens/Eyes)

Depositor

Jean Giacomotto

Article

Tromp et al Front Physiol. 2023 Aug 2;14:1221310. do

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-P2A-APEX2-csChBP

Plasmid

#108877

Purpose

Expression of APEX2 fused to a conditionally stable nanobody against mCherry (includes green cytoplasmic reporter)

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

EGFP-P2A-APEX2-csChBP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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pBAD-jRCaMP1a

Plasmid

#113936

Purpose

Bacterial expression of red fluorescent Ca2+ sensor jRCaMP1a

Depositor

Thomas Hughes, Robert Campbell

Article

Molina et al Biophys J. 2019 May 21;116(10):1873-1886

Insert

jRCaMP1a

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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zbFGF

Plasmid

#12309

Depositor

James Thomson

Article

Ludwig et al Nat Methods. 2006 Aug . 3(8):637-646.

Insert

Fgf2 (fgf2 Zebrafish)

Use

Tags

Expression

Bacterial

Mutation

Ala 11 mutated to Pro, Ser 55 mutated to Asn

Promoter

Available since

Sept. 25, 2006

Availability

Academic Institutions and Nonprofits only

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pBAD-R-GECO1.2

Plasmid

#113937

Purpose

Bacterial expression of red fluorescent Ca2+ sensor R-GECO1.2

Depositor

Thomas Hughes, Robert Campbell

Article

Molina et al Biophys J. 2019 May 21;116(10):1873-1886

Insert

R-GECO1.2

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pSB1C3-H3

Plasmid

#109381

Purpose

Arabinose inducible VioB-mCherry expression (weaker RBS). Used to test burden response in E. coli

Depositor

Tom Ellis

Article

Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

Insert

pARABAD-VioB-mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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acta1-mRuby2-dGBP

Plasmid

#163855

Purpose

Zebrafish muscle specific expression of mRuby2-dVHH[GFP]

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

dVHH[GFP]

Use

In vitro transcription of rna

Tags

Ruby2

Expression

Mutation

Promoter

Available since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pD864-LacZ

Plasmid

#109380

Purpose

Rhamnose inducible LacZ expression. Used to test burden response in E. coli

Depositor

Tom Ellis

Article

Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

Insert

pRHAM-LacZ

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pT3TS-TurboID-EGFP

Plasmid

#163846

Purpose

In vitro transcription of TurboID-EGFP. Parton lab clone JRS

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

TurboID

Use

In vitro transcription of rna

Tags

EGFP

Expression

Mutation

Promoter

Available since

Sept. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5-hygro-eGFP-HEC1-9A

Plasmid

#114028

Purpose

Inducible expression of eGFP-tagged HEC1-9A in FLPin Trex cell lines

Depositor

Geert Kops

Article

Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

Insert

eGFP-HEC1 - 9A (NDC80) (NDC80 Human)

Use

Tags

Expression

Mammalian

Mutation

Hec1 - 9A

Promoter

Available since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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p3E-csGBP

Plasmid

#108891

Purpose

Multisite gateway vector for 3' tagging with a conditionally stable nanobody against GFP

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

csGBP

Use

Multisite gateway

Tags

Expression

Mutation

Promoter

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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LV-TRE-VP16 mouse MyoD-T2A-dsRedExpress2

Plasmid

#60626

Purpose

Expresses VP16 mouse MyoD and dsRedExpress2 in response to doxycycline

Depositor

Charles Gersbach

Article

Kabadi et al ACS Synth Biol. 2014 Dec 10.

Insert

VP16 mouse MyoD

Use

Lentiviral

Tags

Expression

Mutation

Promoter

TRE

Available since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pME-mKate2-P2A-TurboID-NS

Plasmid

#166568

Purpose

Multisite Gateway middle entry clone encoding TurboID with mKate2 marker. Parton lab clone IYN

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

mKate2-P2A-TurboID

Use

Multisite gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge