We narrowed to 2,605 results for: ubiquitin

1. UbcH10 DN AU1

   Plasmid

   #8507

   Depositor

   Joan Ruderman

   Article

   Townsley et al Proc Natl Acad Sci U S A 1997 Mar 18;94(

   Insert

   UbcH10 (UBE2C Human)
   

   Use

   Tags

   AU1 (DTYRYI)

   Expression

   Mammalian

   Mutation

   C114S

   Promoter

   Available since

   June 1, 2005

   Availability

   Academic Institutions and Nonprofits only

2. GP807

   Plasmid

   #164535

   Purpose

   Expresses Smo noK-FLAG-Ub noK

   Depositor

   Gregory Pazour

   Article

   Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

   Insert

   Smoothened noK (Smo Mouse)
   

   Use

   Lentiviral

   Tags

   FLAG_Ub noK

   Expression

   Mutation

   All 21 cytoplasmic Lysines of Smoothened and all…

   Promoter

   CMV

   Available since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTreTight-Htt94Q-CFP

   Plasmid

   #23966

   Depositor

   Nico Dantuma

   Article

   Maynard et al Proc Natl Acad Sci U S A. 2009 Aug 18. 1

   Insert

   N-terminal fragment Huntingtin Q94 (HTT Human)
   

   Use

   Tags

   CFP

   Expression

   Mammalian

   Mutation

   94 polyQ repeat

   Promoter

   Available since

   April 15, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28-mE1

   Plasmid

   #32534

   Depositor

   Jorge Eduardo Azevedo

   Article

   Carvalho et al Mol Biotechnol. 2011 Oct 20.

   Insert

   E1, ubiquitin-activating enzyme (Uba1 Mouse)
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pU6Rosa-CAG-Gal4-Rad18UBD

   Plasmid

   #119012

   Purpose

   Mammalian expression of Rosa26 sgRNA, fusion protein of yeast Gal4 with human Rad18 Ubiquitin binding domain and BFP expression

   Depositor

   Ralf Kuehn

   Article

   Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

   Insert

   Gal4 (GAL4 Budding Yeast)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-Gal4-RNF169UBD

   Plasmid

   #119013

   Purpose

   Mammalian expression of fusion protein of yeast Gal4 with human RNF169 Ubiquitin binding domain and BFP expression

   Depositor

   Ralf Kuehn

   Article

   Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

   Insert

   Gal4 (GAL4 Budding Yeast)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAIP4_2ESK

   Plasmid

   #234232

   Purpose

   Heterologous protein expression of ubiquitin-conjugating enzyme E2 D2 in Escherichia coli

   Depositor

   Christopher Bahl

   Insert

   2ESK (UBE2D2 Human)
   

   Use

   Tags

   10x HisTag-Smt3

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 6, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pAIP4_3ONH

   Plasmid

   #234179

   Purpose

   Heterologous protein expression of ubiquitin-activating enzyme E1-like in Escherichia coli

   Depositor

   Christopher Bahl

   Insert

   3ONH (UBA2 Budding Yeast)
   

   Use

   Tags

   10x HisTag-Smt3

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 6, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pETDuet-AtRBX1-T7-AtSKP1-T7-AtCUL1-S

   Plasmid

   #238003

   Purpose

   Expression AtRBX1-T7, AtSKP1-T7 and AtCUL1-S in in bacterial cells

   Depositor

   Lei Wu

   Article

   Pu et al Biomolecules. 2024 Sep 25;14(10):1209. d

   Inserts

   AtRBX1 (RBX1 Mustard Weed)
   AtSKP1 (SKP1 Mustard Weed)
   AtCUL1 (CUL1 Mustard Weed)
   

   Use

   Tags

   S and T7

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pENTR5'_ubi

    Plasmid

    #27320

    Depositor

    Leonard Zon

    Article

    Mosimann et al Development. 2011 Jan . 138(1):169-77.

    Insert

    zebrafish ubiquitin promoter (ubb Zebrafish)
    

    Use

    Multisite gateway 5' entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 3, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEX4T-E2-25K

    Plasmid

    #63572

    Purpose

    Expression of human Ubiquitin conjugating enzyme E2-25K

    Depositor

    Titia Sixma

    Article

    Pichler et al Nat Struct Mol Biol. 2005 Mar;12(3):264-

    Insert

    UBE2-25K (UBA7 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Isoform 1 (200 resiudes)

    Promoter

    tac

    Available since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA3 MDM2 1-400

    Plasmid

    #45754

    Depositor

    Robert Lefkowitz

    Article

    Shenoy et al Science. 2001 Nov 9;294(5545):1307-13. E

    Insert

    MDM2 1-400 (MDM2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    aa 1-400, lacks catalytic RING domain

    Promoter

    CMV

    Available since

    Aug. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pGEX4T3-Stx3(1-265)

    Plasmid

    #100573

    Purpose

    Expresses cytoplasmic domain of Stx3(1-265) with a GST tag on the N-term.

    Depositor

    Thomas Weimbs

    Insert

    Stx3-1-265 (Stx3 Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    No transmembrane domain, residues 4-264 present.

    Promoter

    tac

    Available since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLSU1/Os-ubi:nnLuz-I{PIV-216}:NosT

    Plasmid

    #212191

    Purpose

    This binary vector expresses mushroom luciferase (nnLuz) containing the PIV intron (216 bp into the gene) with the rice ubiquitin (R. ubi) promoter and UTR

    Depositor

    Wayne Curtis

    Insert

    nnLuz-I{PIV-216}
    

    Use

    Tags

    Expression

    Plant

    Mutation

    PIV intron added at bp 216, eliminates expression…

    Promoter

    Rice ubiquitin

    Available since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pVP16-tev-PRT1

    Plasmid

    #83405

    Purpose

    bacterial expression vector pVP16 containing Arabidopsis thaliana PROTEOLYSIS 1 (PRT1) N-end rule E3 ubiquitin ligase to be used in N-end rule in vitro ubiquitination studies

    Depositor

    Nico Dissmeyer

    Insert

    PROTEOLYSIS1 (PRT1) (PRT1 Mustard Weed, E. coli)
    

    Use

    Tags

    His8, MBP, and tev cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET3a-hUBA1

    Plasmid

    #63571

    Purpose

    Expression of human UBA1 tagged with His

    Depositor

    Titia Sixma

    Article

    Pichler et al Nat Struct Mol Biol. 2005 Mar;12(3):264-

    Insert

    UBA1 (UBA1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Isoform 2 (1018 amino acids)

    Promoter

    T7

    Available since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pENTR221 UBR5 ∆UBR

    Plasmid

    #81063

    Purpose

    Gateway entry vector encoding full length UBR5 W1235L (∆UBR)

    Depositor

    Darren Saunders

    Article

    Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

    Insert

    Ubiquitin protein ligase E3 component N-recognin 5 (UBR5) ∆UBR (UBR5 Human)
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    W1235L (bp g3704t)

    Promoter

    N/A

    Available since

    Jan. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pENTR221 UBR5 ∆HECT

    Plasmid

    #81065

    Purpose

    Gateway entry vector encoding full length UBR5 C2768A (∆HECT)

    Depositor

    Darren Saunders

    Article

    Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

    Insert

    Ubiquitin protein ligase E3 component N-recognin 5 (UBR5) ∆HECT (UBR5 Human)
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    C2768A (bp t8302g and g8303c)

    Promoter

    N/A

    Available since

    Jan. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. Halo-Tev-Flag-Ube2v2

    Plasmid

    #110070

    Purpose

    Express Halo-Tev-Flag-Ube2v2 (human) in mammalian cells

    Depositor

    Yimon Aye

    Article

    Zhao et al ACS Cent Sci. 2018 Feb 28;4(2):246-259.

    Insert

    Halo-Tev-Flag-Ube2v2 (UBE2V2 Human)
    

    Use

    Tags

    Halo-TEV-Flag

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    July 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. WT USP7 catalytic

    Plasmid

    #227201

    Purpose

    Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Article

    Valles et al bioRxiv [Preprint]. 2023 Jan 12:2023.01.

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    aa 207-560

    Promoter

    T7-promoter

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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