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mCh-KIF5A*-strep

Plasmid

#120163

Purpose

Expresses a chimera of mCherry (fluorescent tag), a KIF5A-based anterograde motor and streptavidin

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

kinesin family member 5A (Kif5a Mouse)

Tags

Streptavidin and mCherry

Expression

Mammalian

Mutation

Truncated KIF5A: 1-559 aa

Promoter

CMV

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag PLAU

Plasmid

#20588

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

PLAU (PLAU Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pLentiCRISPRv2-Stuffer-HepmCherry

Plasmid

#192825

Purpose

Contains stuffer sequence into which sgRNAs can be cloned and expresses mCherry from hepatocyte-specific promoter

Depositor

Kristin Knouse

Article

Keys et al Cell Genom. 2022 Dec 14;2(12):100217. do

Insert

mCherry

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

Hepatocyte-specific promoter (HS-CRM8-TTRmin modu…

Available Since

Nov. 23, 2022

Availability

Academic Institutions and Nonprofits only

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BS-Ura3-GFP-Atg8

Plasmid

#69194

Purpose

Single copy integration of GFP-Atg8, use auxotrophic marker URA3(Kluyveromyces lactis)

Depositor

Zhiping Xie

Article

Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

Insert

GFP-Atg8

Tags

EGFP

Expression

Yeast

Available Since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pILGFP3AG4

Plasmid

#185874

Purpose

A plasmid for in vivo gene amplification (HapAmp) at RPL25 locus (~12 copy)

Depositor

Claudia Vickers

Article

Peng et al Nat Commun. 2022 May 24;13(1):2895. doi:

Type

Empty backbone

Expression

Yeast

Mutation

None

Available Since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

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p3xFLAG CMV14 / BRAF-D594G

Plasmid

#131719

Purpose

Mammalian Expression of Flag tagged BRAF

Depositor

Jacques De Grève

Article

Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

Insert

BRAF (BRAF Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

D594G

Promoter

CMV

Available Since

May 19, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-SLCO1B3_STOP

Plasmid

#161366

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLCO1B3 (SLCO1B3 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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AAV r3.45

Plasmid

#233684

Purpose

An evolved Adeno-associated viral variant, enhances gene delivery and gene targeting in neural stem cells

Depositor

David Schaffer

Article

Jang et al Mol Ther. 2011 Apr;19(4):667-75. doi: 10

Insert

Modified AAV2 Rep, Cap

Use

AAV

Mutation

insertion of LATQVGQKTA (where LA and A are linke…

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-E22-ChR2GFP2x

Plasmid

#153436

Purpose

AAV vector to drive the expression of dChr2-GFP-P2A-GFP in PV cortical interneuronsunder the control of the E22 regulatory element

Depositor

Jordane Dimidschstein

Article

Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

Insert

Chr2-GFP-P2A-GFP

Use

AAV

Available Since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-VARNAM2

Plasmid

#195527

Purpose

Red fluorescent, negative response-polarity voltage indicator under the control of CaMKII promoter

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

VARNAM2

Use

AAV

Expression

Mammalian

Mutation

VARNAM SI linker

Promoter

CaMKII

Available Since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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