We narrowed to 4,430 results for: Erf

1. pAM007

   Plasmid

   #170011

   Purpose

   Encodes PslyB-sfgfp reporter and TetR-controlled expression of S. Typhimurium PhoP

   Depositor

   Jeffrey Tabor

   Article

   Brink et al bioRxiv

   Inserts

   Superfolder GFP
   PhoP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PLTetO-1 and PslyB

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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2. Pan-Druggable Cancer Library

   Pooled Library

   #182133

   Purpose

   All-in-one CRISPR/Cas system with 74 individual elements. 63 elements target cancer-related genes with FDA-approved drug treatments, and 3 elements target key medulloblastoma driver genes.

   Depositor

   Prashant Mali

   Article

   Hu et al Biomaterials. 2022 Jan;280:121276. doi:

   Species

   Homo sapiens

   Use

   CRISPR and Lentiviral

   Available since

   Aug. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

3. pTPGI_dCas9

   Plasmid

   #64279

   Purpose

   encodes yeast-optimized dCas9 synthetic transcription factor

   Depositor

   Timothy Lu

   Article

   Farzadfard et al ACS Synth Biol. 2013 Sep 11.

   Insert

   Yeast-optimized dCas9
   

   Use

   Tags

   SV40 NLS

   Expression

   Yeast

   Mutation

   D10A & H840A relative to S. pyogenes Cas9

   Promoter

   pTPGI

   Available since

   May 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSET-dCas9

   Plasmid

   #110183

   Purpose

   Expression of the dCas9 gene in Streptomyces

   Depositor

   Yinhua Lu

   Article

   Zhao et al Biotechnol J. 2018 Jun 3. doi: 10.1002/b

   Insert

   dCas9
   

   Use

   CRISPR; I

   Tags

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Promoter

   ermE*p

   Available since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLKO.1 puro-humanU6-shRNA FASN

   Plasmid

   #82327

   Purpose

   used to silence target human FASN expression via RNA interference (3rd gen lentiviral vector)

   Depositor

   Elizabeth Stoll

   Insert

   shRNA to target Fatty Acid Synthase (FASN) (FASN Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pWZL Hygro-NMYC TRF2 deltaB

   Plasmid

   #16349

   Depositor

   Titia de Lange

   Article

   Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

   Insert

   hTRF2 (TERF2 Human)
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Deleted Basic Domain (aa1-44)

   Promoter

   Available since

   May 14, 2008

   Availability

   Academic Institutions and Nonprofits only

7. Cas9m4

   Plasmid

   #47316

   Purpose

   Cas9 D10A+D839A+H840A+N863A

   Depositor

   George Church

   Article

   Mali et al Nat Biotechnol. 2013 Aug 1. doi: 10.1038

   Insert

   Cas9m4
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. TU#867 (Punc-119sid-1)

   Plasmid

   #25811

   Depositor

   Martin Chalfie

   Article

   Calixto et al Nat Methods. 2010 Jul;7(7):554-9. doi: 1

   Insert

   systemic RNA interference-deficient 1 (sid-1 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Nov. 10, 2010

   Availability

   Academic Institutions and Nonprofits only

9. lentiGuide-Puro PRMT5-CRISPRi

   Plasmid

   #164637

   Purpose

   lentiGuide-Puro backbone with cloned target sequence for human PRMT5. The insert is: 5´- CACCGAGCCGCGTGTCCAGCGGGA-3. sgRNA achieves a downregulation of PRMT5 in combination with dCAS9-KRAB-MCP2

   Depositor

   Günter Schneider

   Insert

   target guide sequence PRMT5 inhibition (PRMT5 Human)
   

   Use

   CRISPR and Lentiviral; Interference

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and EF1A

   Available since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDonor_Tn[sfGFP]

    Plasmid

    #213902

    Purpose

    Mariner transposon expressing sfGFP with kan selection casette

    Depositor

    James Collins

    Article

    English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

    Insert

    mariner transposon with kan resistance and superfolder GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCJH019_SC101_pLAC_Cas12c(4)_Kan

    Plasmid

    #183071

    Purpose

    Wild-type Cas12c_4 in a plasmid containing a SC101 origin and lac promoter for in vivo interference assay.

    Depositor

    Jennifer Doudna

    Article

    Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

    Insert

    Cas12c_4
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Wild-type

    Promoter

    Available since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLPC FLAG hRap1 delta BR delta CT

    Plasmid

    #13257

    Depositor

    Titia de Lange

    Article

    Li et al Mol Biol Cell. 2003 Dec . 14(12):5060-8.

    Insert

    Repressor Activator Protein 1 delta Br delta CT (TERF2IP Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mutation

    contains hRap1 aa2-40 + 96-270 +331-399 and the p…

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

13. pCJH021_SC101_pLAC_Cas12c2_Kan

    Plasmid

    #183072

    Purpose

    Wild-type Cas12c2 (Yan et al., 2019) in a plasmid containing a SC101 origin and lac promoter for in vivo interference assay.

    Depositor

    Jennifer Doudna

    Article

    Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

    Insert

    Cas12c2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. DT042A

    Plasmid

    #159403

    Purpose

    Inducible superfolder GFP (sfGFP) expression, controlled under T7 bacteriophage promoter

    Depositor

    Sheref Mansy

    Article

    Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

    Insert

    pr.T5-LacO-6xHis-sfGFP
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T5 promoter

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSB4A5 JQ929581

    Plasmid

    #38168

    Depositor

    Drew Endy

    Article

    Bonnet et al Proc Natl Acad Sci U S A. 2012 Jun 5;109

    Inserts

    mKate2
    Superfolder GFP
    

    Use

    Synthetic Biology; Biobrick cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

16. MAPTW

    Plasmid

    #205695

    Purpose

    shRNAmir backbone for RNA interference, with mTagBFP2 reporter

    Depositor

    Dapeng Wang

    Article

    Wang et al EMBO Rep. 2022 Apr 5;23(4):e53691. doi:

    Insert

    miR-AB
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    MSCV

    Available since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. p16-1 hTRF2

    Plasmid

    #12299

    Depositor

    Titia de Lange

    Article

    Broccoli et al Nat Genet. 1997 Oct . 17(2):231-5.

    Insert

    hTRF2 (TERF2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A434 deleted; There is a stop codon in the 5'…

    Promoter

    Available since

    Aug. 11, 2006

    Availability

    Academic Institutions and Nonprofits only

18. LI C-D 1-10GFP

    Plasmid

    #54461

    Depositor

    Martin Parniske

    Article

    Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

    Insert

    superfolder GFP 1-10 domain
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSB4A5 JQ929582

    Plasmid

    #38169

    Depositor

    Drew Endy

    Article

    Bonnet et al Proc Natl Acad Sci U S A. 2012 Jun 5;109

    Inserts

    mKate2
    Superfolder GFP
    

    Use

    Synthetic Biology; Biobrick cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 28, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pLPC FLAG hRap1 delta Myb

    Plasmid

    #13253

    Depositor

    Titia de Lange

    Article

    Li et al Mol Biol Cell. 2003 Dec . 14(12):5060-8.

    Insert

    Repressor Activator Protein 1 delta Myb (TERF2IP Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mutation

    contains hRap1 aa2-131 + 184-399 and the point mu…

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only