We narrowed to 8,728 results for: Set

1. AcrIIC1-mCherry chimera #10

   Plasmid

   #128113

   Purpose

   Expresses a chimeric anti-CRISPR protein targeting the N. meningitidis Cas9

   Depositor

   Dominik Niopek

   Article

   Mathony et al Nat Chem Biol. 2020 Apr 13. pii: 10.1038

   Insert

   AcrIIC1-mCherry chimera #10
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFA0057

   Plasmid

   #131784

   Purpose

   Guide RNA (gGFP) and Cas9 expression plasmid for cleaving pFA6 series GFP C-terminal tagging cassettes, including GFP-His3MX6. Used to perform CRISPR-Swap of alleles.

   Depositor

   Frank Albert

   Article

   Lutz et al PLoS Genet. 2019 Nov 18;15(11):e1008375.

   Insert

   20mer GFP cassette guide (gGFP) and 5' sgRNA
   

   Expression

   Bacterial and Yeast

   Available Since

   Oct. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCas9FnCpf1GG

   Plasmid

   #131013

   Purpose

   Backbone plasmid for generating CRISPR arrays for composite array utilized by SpCas9 and FnCas12a using CRATES. Contains a GFP-dropout cassette and two direct repeats.

   Depositor

   Chase Beisel

   Article

   Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

   Inserts

   direct repeat of SpCas9
   GFP expression cassette
   direct repeat of FnCas12a
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. N229A Lo-MetQ

   Plasmid

   #118268

   Purpose

   N229A Mutation in Methionine Binding Protein (MetQ) used to solve crystal structure

   Depositor

   Douglas Rees

   Article

   Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p

   Insert

   MetQ (N229A) (metQ E. coli)
   

   Tags

   10x His Tag and Enterokinase Cleavage Site

   Expression

   Bacterial

   Mutation

   Changed Asparagine (Asn) 229 to Alanine (Ala)

   Promoter

   T7 Promoter

   Available Since

   Jan. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHyg-AID(31-114)

   Plasmid

   #99514

   Purpose

   Truncated C-terminal AID degron cassette with HygR marker for S. cerevisiae

   Depositor

   Helle Ulrich

   Article

   Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

   Insert

   IAA17(31-114) (AXR3 Mustard Weed)
   

   Use

   Pcr template for yeast tagging cassette

   Mutation

   internal inversion of nucleotides 265-312 of the …

   Available Since

   Sept. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMOD_B3200

   Plasmid

   #91079

   Purpose

   Module B, Promoter: none (begins with P2A to be fused to TALE-VPR_1), Gene: TALE-VPR_2 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: HSP

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   TALE-VPR_2 backbone with Esp3I ccdb cassette for repeat cloning
   

   Use

   TALEN

   Promoter

   none (begins with P2A to be fused to TALE-VPR_1)

   Available Since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMOD_B3400

   Plasmid

   #91080

   Purpose

   Module B, Promoter: none (begins with P2A to be fused to TALE-VP64_1), Gene: TALE-VP64_2 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: HSP

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   TALE-VP64_2 backbone with Esp3I ccdb cassette for repeat cloning
   

   Use

   TALEN

   Promoter

   none (begins with P2A to be fused to TALE-VP64_1)

   Available Since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMOD_A3101

   Plasmid

   #91048

   Purpose

   Module A, Promoter: 35S, Gene: TALE-VPR_1 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: none (ends with P2A to be fused to TALE-VPR_ 2)

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   TALE-VPR_1 backbone with Esp3I ccdb cassette for repeat cloning
   

   Use

   TALEN

   Promoter

   35S

   Available Since

   July 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMOD_A3301

   Plasmid

   #91049

   Purpose

   Module A, Promoter: 35S, Gene: TALE-VP64_1 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: none (ends with P2A to be fused to TALE-VP64_ 2)

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   TALE-VP64_1 backbone with Esp3I ccdb cassette for repeat cloning
   

   Use

   TALEN

   Promoter

   35S

   Available Since

   July 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. DCT.VV.Orf199

    Plasmid

    #12887

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jon Yewdell, Jack Bennink

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf199
    

    Expression

    Mammalian

    Available Since

    Dec. 29, 2006

    Availability

    Academic Institutions and Nonprofits only

11. DCT.VV.Orf200

    Plasmid

    #12888

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jon Yewdell, Jack Bennink

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf200
    

    Expression

    Mammalian

    Available Since

    Dec. 29, 2006

    Availability

    Academic Institutions and Nonprofits only

12. DCT.VV.Orf201

    Plasmid

    #12889

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jon Yewdell, Jack Bennink

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf201
    

    Expression

    Mammalian

    Available Since

    Dec. 29, 2006

    Availability

    Academic Institutions and Nonprofits only

13. DCT.VV.Orf202

    Plasmid

    #12890

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jon Yewdell, Jack Bennink

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf202
    

    Expression

    Mammalian

    Available Since

    Dec. 29, 2006

    Availability

    Academic Institutions and Nonprofits only

14. DCT.VV.Orf205

    Plasmid

    #12893

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jon Yewdell, Jack Bennink

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf205
    

    Expression

    Mammalian

    Available Since

    Dec. 29, 2006

    Availability

    Academic Institutions and Nonprofits only

15. DCT.VV.Orf206

    Plasmid

    #12894

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jon Yewdell, Jack Bennink

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf206
    

    Expression

    Mammalian

    Available Since

    Dec. 29, 2006

    Availability

    Academic Institutions and Nonprofits only

16. DCT.VV.Orf208

    Plasmid

    #12896

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jon Yewdell, Jack Bennink

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf208
    

    Expression

    Mammalian

    Available Since

    Dec. 29, 2006

    Availability

    Academic Institutions and Nonprofits only

17. DCT.VV.Orf209

    Plasmid

    #12897

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jon Yewdell, Jack Bennink

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf209
    

    Expression

    Mammalian

    Available Since

    Dec. 29, 2006

    Availability

    Academic Institutions and Nonprofits only

18. DCT.VV.Orf217

    Plasmid

    #12905

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jon Yewdell, Jack Bennink

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf217
    

    Expression

    Mammalian

    Available Since

    Dec. 29, 2006

    Availability

    Academic Institutions and Nonprofits only

19. DCT.VV.Orf219

    Plasmid

    #12907

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jon Yewdell, Jack Bennink

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf219
    

    Expression

    Mammalian

    Available Since

    Dec. 29, 2006

    Availability

    Academic Institutions and Nonprofits only

20. DCT.VV.Orf224

    Plasmid

    #12912

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jon Yewdell, Jack Bennink

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf224
    

    Expression

    Mammalian

    Available Since

    Dec. 29, 2006

    Availability

    Academic Institutions and Nonprofits only