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We narrowed to 18,824 results for: nls

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Showing: 3181 - 3200 of 18824 results

  1. pCAG-hAsCas12a-RVR(S542R/K548V/N552R)-NLS(nucleoplasmin)-3xHA (AAS1065)

    Plasmid
    #114092
    Purpose
    CAG promoter expression plasmid for human codon optimized AsCas12a-RVR(S542R/K548V/N552R) nuclease with C-terminal NLS and HA tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized AsCas12a (S542R/K548V/N552R)
    Tags
    NLS(nucleoplasmin) and 3xHA
    Expression
    Mammalian
    Mutation
    S542R, K548V and N552R
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114092-plasmid-map-sequence-id-230539

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  4. TV_EMPTY-ires-mNeon-nls-p2a-dtr-wpre_pgk-puro

    Plasmid
    #251174
    Purpose
    Empty targeting vector (SapI sites intact) for C-terminal knockin at desired locus to enable reporting of expression by mNeonGreen and depletion of positive cells with DTR
    Depositor
    Hugo Snippert
    Insert
    double SapI flanked ires-mNeon-nls-p2a-dtr-wpre_pgk-puro
    Use
    CRISPR
    Tags
    ires-mNeon-nls-p2a-dtr
    Available since
    Feb. 20, 2026
    Availability
    Academic Institutions and Nonprofits only
    251174-plasmid-map-sequence-id-500685

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  6. pL6puro-JeT-pmRevErbA-mClover3-NLS-Pest

    Plasmid
    #240109
    Purpose
    A lentiviral (yellow-green) fluorescent reporter of circadian rhythm, based on the murine Nr1d1-gene (REVERBa protein), expression enhanced by synthtic JeT promoter.
    Depositor
    Achim Kramer
    Publication
    Gabriel et al bioRxiv 2025.06.30.661926
    Insert
    murine Nr1d1 promoter+mClover3-NLS-Pest-Reporter (Nr1d1 Mouse)
    Use
    Lentiviral
    Mutation
    added JeT Promoter
    Available since
    Aug. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    240109-plasmid-map-sequence-id-481752

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  8. pEG302 22aa SunTag VP64 gRNA4 (FWA)

    Plasmid
    #120249
    Purpose
    CRISPR-Cas9 SunTag system to target VP64 to the FWA locus with a single guide RNA
    Depositor
    Steven Jacobsen
    Publication
    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:
    Insert
    g4_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available since
    April 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    120249-plasmid-map-sequence-id-242035

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  9. pUBC-SV40NLS-HoTag3-Shunzhi12nm-TSapphire

    Plasmid
    #250617
    Purpose
    Mammalian lentiviral vector for the expression of the Sapphire-tagged nuclear localizing 12nm nanoGEMs under the UBC promoter
    Depositor
    Liam Holt
    Publication
    Herzog et al Mol Cell. 2026 Mar 5;86(5):817-833.e7. d
    Insert
    12nm Shunzhi 60mers
    Use
    Lentiviral
    Tags
    4X(GGGGS) linker, HOTag3, SV40NLS, and TSapphire
    Expression
    Bacterial and Mammalian
    Promoter
    pUBC
    Available since
    Jan. 15, 2026
    Availability
    Academic Institutions and Nonprofits only
    250617-plasmid-map-sequence-id-497478

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  11. pCMV-ABE8eWQ

    Plasmid
    #161815
    Purpose
    Expresses ABE8eWQ in mammalian cells
    Depositor
    Sangsu Bae
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    bpNLS-TadA8e(V106W/D108Q)-32AA linker-hSpCas9(D10A)-bpNLS-P2A-EGFP-NLS
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A in S. pyogenes Cas9, TadA mutations describe…
    Available since
    Jan. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    161815-plasmid-map-sequence-id-320654

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  12. pRN3P-NLS-EGFP-Btgh-3NLS

    Plasmid
    #194469
    Purpose
    Encodes for bacterial O-GlcNAcase BtGH84 fused to enhanced GFP and NLSs for effective nuclear localization, in backbone for T3 In Vitro Transcription.
    Depositor
    Mathieu Boulard
    Publication
    Formichetti et al Dev Cell 10 Jan 2024
    Insert
    BtGH84
    Use
    For in vitro transcription from t3 promoter after…
    Tags
    SV40 Nuclear Localization Signal, SV40 Nuclear Lo…
    Expression
    Bacterial
    Available since
    Jan. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    194469-plasmid-map-sequence-id-384873

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  13. NLS-dCas9-HA-NLS-GFP-VP16

    Plasmid
    #183923
    Purpose
    VP16 activation domain coupled to catalytically inactive dCas9 for localization; labeled with GFP
    Depositor
    Karsten Rippe
    Publication
    Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-GFP-VP16
    Use
    CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    June 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    183923-plasmid-map-sequence-id-364153

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  17. pMini-CMV-NLS-dead R-IscB (D60A, H269A)-NLS_T2A_mCherry_U6-ωRNA

    Plasmid
    #246430
    Purpose
    All-in-one plasmid. Expresses R-IscB in mammalian cells. Dead mutations included.
    Depositor
    Ailong Ke
    Publication
    Xu et al Cell. 2025 Aug 18:S0092-8674(25)00854-2.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    O.gue IscB with dead mutations (D60A, H269A) and delta-TID
    mCherry
    ωRNA
    Tags
    SV40 NLS, Thosea asigna virus 2A peptide, and nuc…
    Expression
    Mammalian
    Mutation
    changed Aspartic Acid 60 to Alanine, changed Hist…
    Promoter
    CMV and U6
    Available since
    Oct. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    246430-plasmid-map-sequence-id-490873

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  19. pcDNA4/TO-myc NES-mCE(K294A) NLS-Flag

    Plasmid
    #82469
    Purpose
    Expresses cytoplasmic restricted form of N-terminal myc tagged and C-terminal flag tagged mRNA capping enzyme, inactive form
    Depositor
    Daniel Schoenberg
    Publication
    Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d
    Insert
    mRNA capping enzyme (Rngtt Mouse)
    Tags
    Flag and Myc
    Expression
    Mammalian
    Mutation
    changed Lysine 294 to Alanine
    Promoter
    CMV
    Available since
    Sept. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    82469-plasmid-map-sequence-id-163073

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Showing: 3181 - 3200 of 18824 results