We narrowed to 14,020 results for: Ada;

1. pNCST-AausFP1

   Plasmid

   #129499

   Purpose

   Expresses AausFP1 constitutively in E. coli (most strains)

   Depositor

   Nathan Shaner

   Article

   Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

   Insert

   AausFP1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   synthetic constitutive (stationary phase) promote…

   Available since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJBEI-6410

   Plasmid

   #47049

   Purpose

   BglBrick plasmid (=pBbA5a-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV pathway enzymes to produce limonene from glucose in E. coli

   Depositor

   Taek Soon Lee

   Article

   Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1

   Insert

   Codon-optimized sequences for MEV pathway expression in E. coli to produce limonene
   

   Use

   Synthetic Biology; Bglbrick

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PlacUV5

   Available since

   Sept. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCRISPR-aBEST

   Plasmid

   #131464

   Purpose

   Expression of a Streptomyces codon optimized Cas9n-tadA fusion protein in pGM1190. A to G base editor for actinomycetes

   Depositor

   Tilmann Weber

   Article

   Tong et al Proc Natl Acad Sci U S A. 2019 Oct 8;116

   Inserts

   Streptomyces codon optimized spCas9n (D10A)
   streptomyces codon optimized tadA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   tipA

   Available since

   Nov. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGLO-GFP-1UAG

   Plasmid

   #82500

   Purpose

   Expresses GFP with 1 UAG codon at the amino acid position 3

   Depositor

   Chang Liu

   Article

   Schwessinger et al Integr Biol (Camb). 2016 Apr 18;8(4):542

   Insert

   Green Fluorescent Protein with 1 UAG codon
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Added an UAG codon at the amino acid position 3

   Promoter

   AraBAD

   Available since

   Sept. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLKO.1-TRC.mKO2

   Plasmid

   #85208

   Purpose

   Backbone for lentiviral gene silencing with monomeric Kusabira-Orange2 expression

   Depositor

   Sandra Martha Gomes Dias

   Article

   Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

   Type

   Empty backbone

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   RNA polymerase III promoter for human U6 snRNA fo…

   Available since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a-6XHis-SUMO-SIRT2

   Plasmid

   #102622

   Purpose

   Expresses a truncation (aa38-356) of human SIRT2 isoform 1 in bacteria for purification

   Depositor

   Hening Lin

   Article

   Jing et al Cancer Cell. 2016 Mar 14;29(3):297-310.

   Insert

   SIRT2 (SIRT2 Human)
   

   Use

   Tags

   6XHis-SUMO

   Expression

   Bacterial

   Mutation

   Deleted amino acids 1-37 and 357-389

   Promoter

   Available since

   Feb. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMXs-IP-EGFP-hAtg13

   Plasmid

   #38191

   Depositor

   Noboru Mizushima

   Article

   Hosokawa et al Mol Biol Cell. 2009 Apr . 20(7):1981-91.

   Insert

   autophagy-related genes 13 (ATG13 Human)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 16, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pEGFP-N1_MeCP2(WT)

   Plasmid

   #110186

   Purpose

   Expression of mouse MeCP2 (WT) in mammalian cells

   Depositor

   Adrian Bird

   Article

   Tillotson et al Nature. 2017 Oct 19;550(7676):398-401. d

   Insert

   MeCP2_e2 FL (mouse cDNA) (Mecp2 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available since

   May 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDicAID_nCas9-PmCDA_NptII_Della

   Plasmid

   #91694

   Purpose

   Dicot Target-AID vector expressing dicot-optimized nCas9-PmCDA1 with sgRNA targeting SlDella

   Depositor

   Akihiko Kondo

   Article

   Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Insert

   SpCas9
   

   Use

   Tags

   PmCDA1

   Expression

   Plant

   Mutation

   D10A for nickase Cas9

   Promoter

   PcUbi

   Available since

   June 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBMN YFP-LID

    Plasmid

    #31767

    Depositor

    Thomas Wandless

    Article

    Bonger et al Nat Chem Biol. 2011 Jul 3. doi: 10.1038/

    Insert

    YFP-LID (FKBP1A Human)
    

    Use

    Retroviral

    Tags

    HA and YFP

    Expression

    Mutation

    F36V on FKBP12 and additional 19 aa (TRGVEEVAEGVV…

    Promoter

    LTR

    Available since

    Oct. 4, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSH-EFIRES-B-AtAFB2-mCherry

    Plasmid

    #129718

    Purpose

    AAVS1 safe harbor targeting vector with blasticidin resistance gene expressing AtAFB2-mCherry

    Depositor

    Elina Ikonen

    Article

    Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

    Insert

    AFB2 (AFB2 Mustard Weed)
    

    Use

    CRISPR and TALEN; Human safe harbor locus (a avs1…

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    EF1a

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEN84 - CTCF-AID[71-114]-eGFP-FRT-Puro-FRT targeting construct

    Plasmid

    #86230

    Purpose

    Targeting vector to introduce an AID-eGFP cassette at the mouse CTCF locus using puromycine selection. Auxin-inducible degron system.

    Depositor

    Benoit Bruneau

    Article

    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

    Insert

    AID[71-114]-eGFP
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP Abi1

    Plasmid

    #74905

    Purpose

    Expresses human Abi1 fused to GFP

    Depositor

    Giorgio Scita

    Article

    Innocenti et al Nat Cell Biol. 2004 Apr;6(4):319-27. Epu

    Insert

    Abi1 (ABL1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP-Kif2A

    Plasmid

    #52401

    Purpose

    Expresses GFP-tagged Kif2A in mammalian cells under the CMV promoter

    Depositor

    Ryota Uehara, Gohta Goshima

    Article

    Uehara et al J Cell Biol. 2013 Aug 19;202(4):623-36.

    Insert

    Kif2A (KIF2A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCIneoMyc UNC119B

    Plasmid

    #128333

    Purpose

    Mammalian expression vector of Myc-tagged human UNC119B

    Depositor

    Yutaka Hata

    Article

    Iwasa et al Cancer Sci. 2018 Sep;109(9):2767-2780. d

    Insert

    UNC119B (UNC119B Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3 barr1 flag

    Plasmid

    #14687

    Depositor

    Robert Lefkowitz

    Article

    Luttrell et al Science. 1999 Jan 29. 283(5402):655-61.

    Insert

    beta arrestin 1 (Arrb1 Rat)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 19, 2007

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA5FRT/TO-p47-Strep-HA

    Plasmid

    #113475

    Purpose

    Expression of human p47 with C-terminal strep-HA tag

    Depositor

    Hemmo Meyer

    Article

    Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

    Insert

    p47 (NSFL1C Human)
    

    Use

    Tags

    2xstrep-HA

    Expression

    Mammalian

    Mutation

    wildtype without stop codon

    Promoter

    CMV

    Available since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMT2 Beta1b GFP

    Plasmid

    #89893

    Purpose

    Mammalian expression of Beta1b GFP

    Depositor

    Annette Dolphin

    Article

    Page et al J Biol Chem. 2016 Sep 23;291(39):20402-1

    Insert

    Cacnb1 (Cacnb1 Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    GFP sequence inserted just before the Stop codon

    Promoter

    adenovirus major late promoter

    Available since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMT2 Beta1b mCherry

    Plasmid

    #89892

    Purpose

    Mammalian expression of Beta1b mCherry

    Depositor

    Annette Dolphin

    Article

    Page et al J Biol Chem. 2016 Sep 23;291(39):20402-1

    Insert

    Cacnb1 (Cacnb1 Rat)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    mCherry sequence inserted just before the Stop co…

    Promoter

    adenovirus major late promoter

    Available since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET20b_Pa-PiuA

    Plasmid

    #128955

    Purpose

    expresses Pseudomonas aeruginosa PiuA in E. coli

    Depositor

    James Naismith

    Article

    Moynie et al Antimicrob Agents Chemother. 2017 Mar 24

    Insert

    iron transport outer membrane receptor (PiuA) (PA4514 Pseudomonas aeruginosa PAO1)
    

    Use

    Tags

    6xHis and pelB leader

    Expression

    Bacterial

    Mutation

    Signal peptide sequence has been removed

    Promoter

    T7

    Available since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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