We narrowed to 12,270 results for: cel.2

1. p3xFLAG-CMV-14-Mfn1-T564A

   Plasmid

   #191978

   Purpose

   To produce mutants mimicking dephosphorylated Mfn1

   Depositor

   Luca Scorrano

   Article

   Pyakurel et al Mol Cell. 2015 Apr 16;58(2):244-54. doi:

   Insert

   Mfn1 T564A (Mfn1 Mouse)
   

   Expression

   Mammalian

   Mutation

   T564A

   Available Since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. p3xFLAG-CMV-14-Mfn1-T564D

   Plasmid

   #191979

   Purpose

   To produce mutants mimicking phosphorylated Mfn1

   Depositor

   Luca Scorrano

   Article

   Pyakurel et al Mol Cell. 2015 Apr 16;58(2):244-54. doi:

   Insert

   Mfn1 T564D (Mfn1 Mouse)
   

   Expression

   Mammalian

   Mutation

   T564D

   Available Since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-LifeAct-FRB-DMVF-181-N

   Plasmid

   #87610

   Purpose

   FRB-tagged N-terminal Dronpa fragment for BiFC; localizes to actin cytoskeleton.

   Depositor

   Jin Zhang

   Article

   Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

   Insert

   LifeAct-FRB-DMVF181-N
   

   Tags

   17-aa sequence from Abp140 (aka "Lifeact&quo…

   Expression

   Mammalian

   Promoter

   CMV

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAFNF-Venus-Actb

   Plasmid

   #169788

   Purpose

   An actin FRET sensor. Must be expressed with ECFP-actin for FRET.

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   Actb (Actb Mouse)
   

   Tags

   Venus

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJCX3-ACTG2_R257C

   Plasmid

   #228911

   Purpose

   Expresses mutant R257C smooth muscle actin gamma-2 in human cells

   Depositor

   Roberto Dominguez

   Insert

   Actin gamma 2, smooth muscle (ACTG2 Human)
   

   Tags

   HIS-FLAG-TEV

   Expression

   Bacterial and Mammalian

   Mutation

   R257C

   Promoter

   CMV

   Available Since

   Jan. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJCX3-ACTG2_WT

   Plasmid

   #228910

   Purpose

   Expresses wildtype smooth muscle actin gamma-2 in human cells

   Depositor

   Roberto Dominguez

   Insert

   Actin gamma 2, smooth muscle (ACTG2 Human)
   

   Tags

   HIS-FLAG-TEV

   Expression

   Bacterial and Mammalian

   Mutation

   WT

   Promoter

   CMV

   Available Since

   Jan. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR221_SMAD2_HAe2_Bsd

   Plasmid

   #167166

   Purpose

   Blasticidin selection cassette flanked by homology arms from human SMAD2 exon 2 for use in creating CRISPR-Cas9 mediated gene knockout.

   Depositor

   Alexander Loewer

   Insert

   Repair template to knockout SMAD2 by inserting a blasticidin resistance cassette (SMAD2 Human)
   

   Use

   Repair template for gene editing in human cells

   Tags

   blasticidin

   Available Since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. human KANK2-A670V Ankyrin Repeats pET151

   Plasmid

   #164055

   Purpose

   Expresses human KANK2 Ank domain - A670V mutant in bacterial cells

   Depositor

   Ben Goult

   Insert

   Kidney Ankyrin Repeat-Containing Protein 2 A670V mutant (KANK2 Human)
   

   Tags

   His-tag, TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSicoR-RNF40i1

   Plasmid

   #59594

   Purpose

   Expression of shRNA against human RNF40

   Depositor

   Miguel Ramalho-Santos

   Article

   Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

   Insert

   RNF40 (RNF40 Human)
   

   Use

   RNAi

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 26, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pCF730

    Plasmid

    #121668

    Purpose

    EF1a-ProCas9-Flavi-P2A-Puro

    Depositor

    David Savage

    Article

    Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

    Insert

    Cas9(C)
    

    Use

    Lentiviral

    Promoter

    EF-1a core promoter

    Available Since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSicoR-TTF2i2

    Plasmid

    #59623

    Purpose

    Expression of shRNA against human TTF2

    Depositor

    Miguel Ramalho-Santos

    Article

    Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

    Insert

    TTF2 (TTF2 Human)
    

    Use

    RNAi

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pSicoR-TTF2i1

    Plasmid

    #59622

    Purpose

    Expression of shRNA against human TTF2

    Depositor

    Miguel Ramalho-Santos

    Article

    Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

    Insert

    TTF2 (TTF2 Human)
    

    Use

    RNAi

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pSicoR-RNF40i2

    Plasmid

    #59595

    Purpose

    Expression of shRNA against human RNF40

    Depositor

    Miguel Ramalho-Santos

    Article

    Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

    Insert

    RNF40 (RNF40 Human)
    

    Use

    RNAi

    Expression

    Mammalian

    Promoter

    u

    Available Since

    Sept. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pCF708

    Plasmid

    #121685

    Purpose

    EF1a-dTEV-protease-mTagBFP2

    Depositor

    David Savage

    Article

    Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

    Insert

    TEV protease
    

    Use

    Lentiviral

    Promoter

    EF-1a core promoter

    Available Since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEMT-T-HSV2.3 TCR alpha chain

    Plasmid

    #74076

    Purpose

    gBT-I TCR a chain cDNA in pGEM-T

    Depositor

    Francis R. Carbone

    Article

    Mueller et al Immunol Cell Biol. 2002 Apr;80(2):156-63

    Insert

    TCR alpha clone HSV2.3 (Tcra Mouse)
    

    Expression

    Bacterial

    Available Since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCF204

    Plasmid

    #121656

    Purpose

    U6-sgRNA EFS-Cas9-wt-P2A-Puro

    Depositor

    David Savage

    Article

    Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

    Insert

    Cas9
    

    Use

    Lentiviral

    Promoter

    U6

    Available Since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSicoR-PLCE1i1

    Plasmid

    #59628

    Purpose

    Expression of shRNA against human PLCE1

    Depositor

    Miguel Ramalho-Santos

    Article

    Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

    Insert

    PLCE1 (PLCE1 Human)
    

    Use

    RNAi

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Oct. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pSicoR-TTF1i1

    Plasmid

    #59620

    Purpose

    Expression of shRNA against human TTF1

    Depositor

    Miguel Ramalho-Santos

    Article

    Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

    Insert

    TTF1 (TTF1 Human)
    

    Use

    RNAi

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pCF226

    Plasmid

    #121667

    Purpose

    EFS-Cas9-wt-P2A-Puro

    Depositor

    David Savage

    Article

    Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

    Insert

    cas9
    

    Use

    Lentiviral

    Promoter

    EF-1a core promoter

    Available Since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSicoR-PTPRKi2

    Plasmid

    #59611

    Purpose

    Expression of shRNA against human PTPRK

    Depositor

    Miguel Ramalho-Santos

    Article

    Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

    Insert

    PTPRK (PTPRK Human)
    

    Use

    RNAi

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only