We narrowed to 32,170 results for: uros

1. pMSCV-FLAG-2xSTREP-CCND2(T280A)-Puro

   Plasmid

   #172625

   Purpose

   Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D2(T280A) and a puromycin resistance marker in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CCND2 (CCND2 Human)
   

   Use

   Retroviral

   Tags

   FLAG-2xSTREP

   Expression

   Mammalian

   Mutation

   Thr280Ala

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLVX-EF1a-EGFP-TA Bik-IRES-Puromycin

   Plasmid

   #133026

   Purpose

   Expresses EGFP-tagged tail anchor sequence of Bik

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   Bcl-2-interacting killer (BIK Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-110

   Promoter

   Human elongation factor 1 alpha

   Available Since

   Feb. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. nonphosphorylatable Cenp-B-targted Aurora B FRET sensor

   Plasmid

   #45230

   Depositor

   Michael Lampson

   Article

   Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

   Insert

   nonphos Cenp-B targeted Aurora B FRET sensor
   

   Expression

   Mammalian

   Mutation

   t-a mutation in substrate sequence

   Available Since

   July 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. hPlekhm1-C/pIRES puro Glue (S778-A1056)

   Plasmid

   #73456

   Purpose

   Express human C-terminal part (Q778-A1056) Plekhm1 in mammalian cells

   Depositor

   Paul Odgren

   Article

   Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

   Insert

   Plekhm1 (PLEKHM1 Human)
   

   Tags

   CBP tag, HA tag, and strep tag

   Expression

   Mammalian

   Available Since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pLenti-VQRRA-P2A-GFP-PGK-Puro

   Plasmid

   #110864

   Purpose

   Lentiviral vector for constitutive expression of Cas9-VQRRA-P2A-GFP in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-VQRRA
   

   Use

   Lentiviral

   Tags

   FLAG

   Mutation

   D1135V, R1335Q, T1337R and NLS sequence at the N-…

   Promoter

   EF1s

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. CAG-xFNLS-T2A-EGFP-ires-puro

   Plasmid

   #121171

   Purpose

   CAG promoter driven expression of xFNLS BE3 base editor, EGFP and Puromycin resistance.

   Depositor

   Timo Otonkoski

   Insert

   xFNLS-T2A-EGFP-ires-puro
   

   Use

   CRISPR

   Tags

   T2A-EGFP-ires-puro

   Expression

   Mammalian

   Available Since

   July 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSUPER-retro-puro-tetO-Mek1-shRNA

   Plasmid

   #53179

   Purpose

   Expresses shRNA against mouse Mek1 from puromycin resistance retroviral vector

   Depositor

   Christopher Counter

   Article

   Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

   Insert

   MEK1 (Map2k1 Mouse)
   

   Use

   RNAi and Retroviral

   Expression

   Mammalian

   Available Since

   June 19, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pLenti-EF1a-Ly6G-P2A-PuroR-HA-miR30

   Plasmid

   #163359

   Purpose

   Lentiviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase, with expression of a Ly6G surface marker and a puromycin selection marker.

   Depositor

   Louis Ates, Teunis Geijtenbeek

   Article

   van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

   Insert

   Ly-6G (Ly6g Mouse)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   EF1

   Available Since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. Opie2-NeoR[traF]+Opie2-PuroR[dsxM]

   Plasmid

   #131618

   Purpose

   Expresses a functional NeoR only in females, a functional PuroR only in males, and dsRed in both genders.

   Depositor

   Omar Akbari

   Article

   Kandul et al Nat Commun. 2020 Apr 30;11(1):2106. doi:

   Inserts

   PuroR[dsxM]
   NeoR[traF]
   

   Expression

   Insect

   Promoter

   Opie2

   Available Since

   May 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. MSCV-BRD9_BRG1BD-PGK-Puro-IRES-GFP

    Plasmid

    #75121

    Purpose

    Retroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the BRG1 bromodomain

    Depositor

    Christopher Vakoc

    Article

    Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

    Insert

    BRD9 (BRD9 Human)
    

    Use

    Retroviral

    Mutation

    Bromodomain replaced with the bromodomain of BRG1

    Available Since

    June 30, 2016

    Availability

    Academic Institutions and Nonprofits only

11. MSCV-BRD9_BRD1BD-PGK-Puro-IRES-GFP

    Plasmid

    #75119

    Purpose

    Retroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the BRD1 bromodomain

    Depositor

    Christopher Vakoc

    Article

    Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

    Insert

    BRD9 (BRD9 Human)
    

    Use

    Retroviral

    Mutation

    Bromodomain replaced with the bromodomain of BRD1

    Available Since

    May 18, 2016

    Availability

    Academic Institutions and Nonprofits only

12. 1070 pBabe puroL PTEN 360-364A A4

    Plasmid

    #10943

    Depositor

    William Sellers

    Insert

    PTEN (PTEN Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    360-364A (SSSTS to AAAAA); A4: S380A, T382A, T383…

    Available Since

    Jan. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

13. pBabe 3XFLAG-S37A-GATA6-3XAU1 puro

    Plasmid

    #72608

    Purpose

    For mammalian expression of an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human GATA6 with a single serine#37 to alanine mutation

    Depositor

    Kevin Janes

    Article

    Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

    Insert

    GATA binding protein 6 (GATA6 Human)
    

    Use

    Retroviral

    Tags

    3XAU1 and 3XFLAG

    Expression

    Mammalian

    Mutation

    changed serine37 to alanine

    Available Since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

14. integrin beta6 Y762F, Y774F pBabe puro

    Plasmid

    #13598

    Depositor

    Dean Sheppard

    Insert

    integrin beta 6 Y762F Y774F (ITGB6 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    Y762F Y774F

    Available Since

    Jan. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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15. integrin alpha9 / alpha5 EGFP pBabe puro

    Plasmid

    #13608

    Depositor

    Dean Sheppard

    Insert

    integrin alpha 9 / alpha 5 (ITGA9 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Integrin alpha 9 chimera containing the cytoplasm…

    Available Since

    Jan. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-VRERRA-P2A-GFP-PGK-Puro

    Plasmid

    #110865

    Purpose

    Lentiviral vector for constitutive expression of Cas9-VRERRA-P2A-GFP in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-VRERRA
    

    Use

    Lentiviral

    Tags

    FLAG

    Mutation

    D1135V, G1218R, R1335E, T1337R, and NLS sequence …

    Promoter

    EF1s

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. N112 nLV EF-1a-PGK-Puro Gal4-HP1a CS

    Plasmid

    #43969

    Depositor

    Jerry Crabtree

    Article

    Hathaway et al Cell. 2012 Jun 22;149(7):1447-60. doi: 1

    Insert

    Cbx5 (Cbx5 Mouse)
    

    Use

    Lentiviral

    Tags

    Gal4/DBD

    Mutation

    N-term chromo domain removed

    Promoter

    EF-1a

    Available Since

    June 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. MSCV-6NBC-GFP-PGK-Puro-Barcode83

    Plasmid

    #83760

    Purpose

    individual 6N barcode to make barcoded cell line variants

    Depositor

    Monte Winslow

    Article

    Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

    Insert

    Barcode83
    

    Use

    Retroviral

    Available Since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. 1038 pBabe puroL PTEN 360-364A

    Plasmid

    #10940

    Depositor

    William Sellers

    Insert

    PTEN (PTEN Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    360-364A (SSSTS to AAAAA)

    Available Since

    Jan. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

20. hPlekhm1-C/pIRES puro Glue (H987-end)

    Plasmid

    #73458

    Purpose

    Express human C-terminal part (H987-end) Plekhm1 in mammalian cells

    Depositor

    Paul Odgren

    Article

    Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

    Insert

    Plekhm1 (PLEKHM1 Human)
    

    Tags

    CBP tag, HA tag, and strep tag

    Expression

    Mammalian

    Available Since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only