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MTK234_058

Plasmid

#123984

Purpose

Encodes the spCas9 Safetargeting Chr11 gRNA expression cassette as a type 234 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

sg Safetargeting Chr11

Expression

Mammalian

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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MTK234_038

Plasmid

#123917

Purpose

Encodes the spCas9 pSV40 (site 1) gRNA expression cassette as a type 234 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

sgRNA-pSV40

Expression

Mammalian

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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MTK234_043

Plasmid

#123913

Purpose

Encodes the spCas9 pSV40 (site 2) gRNA expression cassette as a type 234 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

sgRNA-pSV40

Expression

Mammalian

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pET_4E1H.95

Plasmid

#155213

Purpose

Expresses 4E1H.95 de novo protein in E.coli

Depositor

Bruno Correia

Article

Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

Insert

4E1H.95

Tags

6x His Tag

Expression

Bacterial

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pET_4H.01

Plasmid

#155210

Purpose

Expresses 4H.01 de novo designed protein in E.coli

Depositor

Bruno Correia

Article

Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

Insert

4H.01

Tags

6x His Tag

Expression

Bacterial

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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4E1H.95_LUMABS

Plasmid

#155208

Purpose

Expresses 4E1H.95_LUMABS sensor in E.coli

Depositor

Bruno Correia

Article

Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

Insert

4E1H.95_LUMABS

Tags

6x His Tag

Available Since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pET_3E2H.37

Plasmid

#155211

Purpose

Expresses 3E2H.37 de novo designed protein in E.coli

Depositor

Bruno Correia

Article

Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

Insert

3E2H.37

Tags

6x His Tag

Expression

Bacterial

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pET_3H1L_02.395

Plasmid

#155198

Purpose

Expresses 3H1L_02.395 in E.coli

Depositor

Bruno Correia

Article

Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

Insert

3H1L_02.395

Tags

6x His Tag

Expression

Bacterial

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-Avi-Coh (E154C)-HIS

Plasmid

#153447

Purpose

E. coli expression of Rc. CohE construct with E154C mutation.

Depositor

Michael Nash

Article

Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

Insert

Rc.CohE

Tags

6xHis and Avi-tag

Expression

Bacterial

Mutation

E154C mutation on CohE

Promoter

T7 promoter

Available Since

July 23, 2020

Availability

Academic Institutions and Nonprofits only

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MTK234_015

Plasmid

#123991

Purpose

Encodes the spCas9 sgRNA1 hROSA26 (site 4) gRNA expression cassette as a type 234 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

hROSA26 sg4

Expression

Mammalian

Available Since

Feb. 24, 2020

Availability

Academic Institutions and Nonprofits only

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MTK8_005

Plasmid

#123885

Purpose

Encodes the F1 Kanamycin resistance cassette as a type 8 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

F1-KanR-ColE1

Expression

Mammalian

Available Since

Feb. 17, 2020

Availability

Academic Institutions and Nonprofits only

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MTK0_021

Plasmid

#123936

Purpose

Encodes the spacer TU with connectors L3 R4 as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

TUL3R4 spacer

Expression

Mammalian

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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MTK234_047

Plasmid

#124055

Purpose

Encodes the spCas9 mScarlet (site 2) gRNA expression cassette as a type 234 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

mScarlet sgRNA 2

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK234_048

Plasmid

#124056

Purpose

Encodes the spCas9 mScarlet (site 3) gRNA expression cassette as a type 234 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

mScarlet sgRNA 3

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK234_049

Plasmid

#124057

Purpose

Encodes the spCas9 mScarlet (site 4) gRNA expression cassette as a type 234 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

mScarlet sgRNA 4

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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ARB340

Plasmid

#124023

Purpose

Encodes TU with connectors LS R1 and an A4 insulator, and pEF1a expressing nuclear iRFP713

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

LS-A4_EF1a-NLS::iRFP713-bgPA_R1

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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ARB341

Plasmid

#124024

Purpose

Encodes TU with connectors LS R1 and an A4 insulator, and pEF1a expressing nuclear tagBFP

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

LS-A4_EF1a-NLS::tagBFP-bgPA_R1

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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ARB344

Plasmid

#124027

Purpose

Encodes TU with connectors L1 R2 and an B1 insulator, and pCMV expressing cytoplasmic iRFP713

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

L1-B1_CMV-NES::iRFP713-rglPA_R2

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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ARB345

Plasmid

#124028

Purpose

Encodes TU with connectors L1 R2 and an B1 insulator, and pCMV expressing cytoplasmic tagBFP

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

L1-B1_CMV-NES::tagBFP-rglPA_R2

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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ARB346

Plasmid

#124029

Purpose

Encodes TU with connectors L1 R2 and an B1 insulator, and pCMV expressing cytoplasmic mAzamiGreen

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

L1-B1_CMV-NES::mAzamiGreen-rglPA_R2

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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