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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,017 results for: donor

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Showing: 3241 - 3260 of 9017 results

  7. pJ204-loxP-EF1a-tagBFP-HygR-lox2272-BGHpA-siteT9-HDR

    Plasmid
    #154827
    Purpose
    Landing pad for Cre/lox-based recombinase-mediated cassette exchange (RMCE) in CHO cells, donor plasmid for CRISPR/Cas9-mediated targeted integration in CHO cells (site T9)
    Depositor
    Lise Marie Grav
    Publication
    Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    TagBFP
    HygR
    ZsGreen1-DR
    Use
    CRISPR and Cre/Lox
    Tags
    PEST
    Expression
    Mammalian
    Promoter
    CMV, EF1a, and SV40 early promoter
    Available since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    154827-plasmid-map-sequence-id-306202

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  11. pHX_HDR_hPAX7_IRES-EGFP-PGK-Neo

    Plasmid
    #160458
    Purpose
    Homology-directed recombination donor vector for EGFP reporter knock-in to human PAX7 3' UTR
    Depositor
    April Pyle
    Publication
    Xi et al STAR Protoc. 2020 Nov 5;1(3):100158. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    PAX7 Left/5' Homology Arm (PAX7 Human)
    PAX7 Right/3' Homology Arm (PAX7 Human)
    Use
    Insertion of a fluorescent reporter through homol…
    Available since
    Nov. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    160458-plasmid-map-sequence-id-316796

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  12. FOR human NANOS3 TALEN

    Plasmid
    #59722
    Purpose
    TALEN expressing vector used to allow Knock-in of NANOS3-P2A-mCherry knockin donor plasmid (addgene #59721)
    Depositor
    Jacob Hanna
    Publication
    Irie et al Cell. 2015 Jan 15;160(1-2):253-68. doi:
    Insert
    HD HD NG NH HD NG HD NG HD HD HD NG HD HD NI NG
    Use
    TALEN
    Expression
    Mammalian
    Promoter
    CAGGS
    Available since
    Dec. 29, 2014
    Availability
    Academic Institutions and Nonprofits only

  13. REV human NANOS3 TALEN

    Plasmid
    #59723
    Purpose
    TALEN expressing vector used to allow Knock-in of NANOS3-P2A-mCherry knockin donor plasmid (addgene #59721)
    Depositor
    Jacob Hanna
    Publication
    Irie et al Cell. 2015 Jan 15;160(1-2):253-68. doi:
    Insert
    NG NH HD HD HD NI HD HD NG NH NG NI NH NH HD NI
    Use
    TALEN
    Expression
    Mammalian
    Promoter
    CAGGS
    Available since
    Dec. 29, 2014
    Availability
    Academic Institutions and Nonprofits only

  14. pTF-lacZ-rfp

    Plasmid
    #153037
    Purpose
    CRISPR-Cas12a genome editing in E. coli. Contains a constitutively CRISPR array targeting the lacZ gene in E. coli genome and donor DNA to insert an constitutively expressed rfp reporter gene.
    Depositor
    Nigel Scrutton
    Publication
    Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.
    Insert
    CRISPR array
    Expression
    Bacterial
    Available since
    May 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    153037-plasmid-map-sequence-id-318855

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  16. mUAVbla:tetR 5′

    Plasmid
    #149379
    Purpose
    mUAVbla containing a tetracycline resistance (tetA) cassette for building Gene Doctoring donor plasmids from pDOC-GG, using BsaI Golden Gate assembly with GGAG and CGCT overhangs
    Depositor
    Mark Pallen
    Publication
    Thomson et al BMC Biotechnol. 2020 Oct 7;20(1):54. doi
    Insert
    tetA
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    149379-plasmid-map-sequence-id-308791

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Showing: 3241 - 3260 of 9017 results