169,099 results

1. pET29-bdSUMO-Anti-ALFA nanobody

   Plasmid

   #212311

   Purpose

   Expression of anti-ALFA nanobody

   Depositor

   Arthur Laganowsky

   Article

   Stover et al J Struct Biol X. 2024 Feb 2;9:100097. do

   Insert

   anti-ALFA nanobody
   

   Tags

   bdSUMO

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. EGFP-DCX

   Plasmid

   #32852

   Depositor

   Joseph Gleeson

   Article

   Tanaka et al J Cell Biol. 2004 Jun 7. 165(5):709-21.

   Insert

   DCX (DCX Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. Murine cell surface protein knockout (McspKO) library

   Pooled Library

   #200445

   Purpose

   Knockout library targeting mouse cell surface genes.

   Depositor

   Junjie Chen

   Article

   Wang et al Proc Natl Acad Sci U S A. 2022 Jun 21;11

   Expression

   Mammalian

   Species

   Mus musculus

   Use

   CRISPR, Lentiviral, and Mouse Targeting

   Available Since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

4. pTUB1:OsTIR1-3FLAG, SAG1:CAT

   Plasmid

   #87258

   Purpose

   TIR1-3FLAG fusion driven by a TUB1 promoter with a CAT drug selectable marker. The TIR1 CDS from Oryza sativa was codon optimized for T. gondii expression.

   Depositor

   David Sibley

   Article

   Brown et al MBio. 2017 May 2;8(3). pii: e00375-17. d

   Inserts

   TIR1
   CAT
   

   Use

   Toxoplasma expression

   Tags

   3FLAG

   Mutation

   Codon optimized for Toxoplasma gondii expression

   Promoter

   TgSAG1 361 bp and TgTUB1 2717 bp

   Available Since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-PGK-Neo-PIP-FUCCI

   Plasmid

   #118616

   Purpose

   Lentiviral expression of the cell cycle marker PIP-FUCCI

   Depositor

   Jean Cook

   Article

   Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

   Insert

   PIP-FUCCI
   

   Use

   Lentiviral

   Tags

   mCherry and mVenus

   Expression

   Mammalian

   Promoter

   hPGK

   Available Since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPB_TRE3G_dCas9-5xGCN4_Hygro

   Plasmid

   #235572

   Purpose

   Dox-inducible expression of dCas9-GCN4 & genomic integration

   Depositor

   Jamie Hackett

   Article

   Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

   Insert

   dCas9-5xGCN4
   

   Use

   CRISPR

   Mutation

   D10A; H840A

   Available Since

   April 29, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLVX-puro-cOVA-IRES-BFP

   Plasmid

   #135074

   Purpose

   Expresses chicken cytoplasmic ovalbumin in lentiviral vector with puromycin resistance. cOVA is coupled with BFP fluorescent reporter using IRES.

   Depositor

   Maria Castro

   Insert

   cOVA-IRES-BFP (OVAL Chicken)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLyGo-Kp-1

   Plasmid

   #163143

   Purpose

   pLyGo cloning vector for a sequence of interest (LPMO) in K. phaffii. Vector encoding the alfa-MF pre-sequence and the LyGo cassette (SapI-ccdB-SapI)

   Depositor

   Morten Norholm

   Article

   Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Yeast

   Promoter

   AOX1

   Available Since

   Aug. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHRdSV40-K560-24xGCN4_v4

   Plasmid

   #72229

   Purpose

   A fusion of the Kinesin-1 motor domain (amino acids 1-560) to the v4 SunTag peptide array

   Depositor

   Ron Vale

   Article

   Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

   Insert

   K560 kinesin
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Feb. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. ppyCAG_RNaseH1_D210N

    Plasmid

    #111904

    Purpose

    Express D210N mutated RNASEH1 which abolishes the catalytic activity of RNASEH1

    Depositor

    Xiang-Dong Fu

    Article

    Chen et al Mol Cell. 2017 Nov 16;68(4):745-757.e5.

    Insert

    RNASEH1 (RNASEH1 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Changed D 210 to N

    Available Since

    July 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCS2-UTX-F-MT2

    Plasmid

    #40619

    Purpose

    Expression of enzyme-dead UTX in mammalian cells

    Depositor

    Kai Ge

    Article

    Wang et al Proc Natl Acad Sci U S A. 2012 Sep 4.

    Insert

    UTX (KDM6A Human)
    

    Tags

    6xMyc and Flag

    Expression

    Mammalian

    Mutation

    changed Histidine 1146 and Glutamic Acid 1148 to …

    Promoter

    CMV

    Available Since

    Nov. 5, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pEGFP-N1-TFEB

    Plasmid

    #38119

    Purpose

    Mammalian expression of Transcription factor EB fused to EGFP

    Depositor

    Shawn Ferguson

    Article

    Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

    Insert

    TFEB (TFEB Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    deleted stop codon

    Promoter

    CMV

    Available Since

    July 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. HDM_RSV_B1_F

    Plasmid

    #237353

    Purpose

    Expression plasmid for RSV B1 F

    Depositor

    Jesse Bloom

    Article

    Simonich et al J Virol. 2025 Jul 3:e0053125. doi: 10.11

    Insert

    RSV B1 F human codon optimized from Genbank Accesion AF013254
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. 35S-VP16-GWY/pAM

    Plasmid

    #201234

    Purpose

    Used to produce and express in planta C-terminal fusion proteins with the VP16 activation domain

    Depositor

    Michael Ayliffe

    Article

    Chen et al Plant J. 2023 Jun;114(6):1209-1226. doi:

    Insert

    35S-VP16-GWY
    

    Expression

    Plant

    Promoter

    CaMV 35S

    Available Since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET-TRSter

    Plasmid

    #78865

    Purpose

    Expresses rat Thioredoxin Reductase using an open reading frame in fusion with a mutated E. coli-type SECIS element

    Depositor

    Elias Arnér

    Article

    Arner et al J Mol Biol. 1999 Oct 8;292(5):1003-16.

    Insert

    Thioredoxin Reductase (Txnrd1 Rat)
    

    Tags

    Mutated E. coli-type SECIS element (3’ end of ins…

    Expression

    Bacterial

    Promoter

    T7lac

    Available Since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV.CMV.SV40.THBS1-HA.SV40(polyA)

    Plasmid

    #154850

    Purpose

    Expresses murine Thrombospondin-1 (THBS1) protein with HA-tag at C-terminus

    Depositor

    Kevin Park

    Article

    Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

    Insert

    Thrombospondin-1 (Thbs1 Mouse)
    

    Use

    AAV

    Tags

    HA-tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. 35S-GAL4BD-GWY/pAM

    Plasmid

    #201233

    Purpose

    Used to produce and express in planta N-terminal fusion proteins with the GAL4 BD

    Depositor

    Michael Ayliffe

    Article

    Chen et al Plant J. 2023 Jun;114(6):1209-1226. doi:

    Insert

    35S-GAL4BD-GWY
    

    Expression

    Plant

    Promoter

    CaMV 35S

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTA7002-avrPto

    Plasmid

    #49156

    Purpose

    Plant transformation binary vector for Dexamethasone inducible expression of the type III effector gene avrPto

    Depositor

    Sheng Yang He

    Article

    Hauck et al Proc Natl Acad Sci U S A. 2003 Jul 8;100

    Insert

    avrPto
    

    Use

    Plant transformation binary vector

    Promoter

    dexamethasone inducible

    Available Since

    April 14, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pF3BGX-T2A-lexAp65

    Plasmid

    #138393

    Purpose

    RMCE vector for generating C-terminal T2A-lexA-p65 fusion

    Depositor

    Shu Kondo

    Article

    Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

    Insert

    T2A-lexAp65
    

    Expression

    Insect

    Available Since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-Ef1a-fDIO-ChrimsonR-tdTomato (AAV9)

    Viral Prep

    #171027-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV-Ef1a-fDIO-ChrimsonR-tdTomato (#171027). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO-ChrimsonR-tdTomato plasmid DNA. Flp-dependent, Ef1a-driven expression of ChrimsonR-tdTomato fusion for optogenetic activation. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Patricia Jensen

    Article

    Sciolino et al Sci Adv. 2022 Aug 19;8(33):eabn9134. doi

    Promoter

    EF1a

    Tags

    tdTomato

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only