164,874 results

1. Human SAM library in lenti sgRNA(MS2)_puro backbone

   Pooled Library

   #1000000074

   Purpose

   Genome-wide CRISPR gRNA pooled library for activation of mouse genes

   Depositor

   Feng Zhang

   Available since

   March 17, 2016

   Availability

   Academic Institutions and Nonprofits only

2. RHO3

   Bacterial Strain

   #124700

   Purpose

   E. coli mobilizer strain that facilitates conjugation of mobilizable plasmids by using metabolic counter-selection against the donor strain.

   Depositor

   Herbert Schweizer

   Article

   Lopez et al Appl Environ Microbiol. 2009 Oct;75(20):

   Bacterial Resistance

   Dap (400 µg/ml)

   Available since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

3. pMito-mCherry-FRB

   Plasmid

   #59352

   Purpose

   Expresses mCherry-tagged MitoTrap in mammalian cells.

   Depositor

   Stephen Royle

   Article

   Cheeseman et al J Cell Sci. 2013 May 1;126(Pt 9):2102-13

   Type

   Empty backbone

   Use

   Tags

   FRB and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. sfGFP-C1

   Plasmid

   #54579

   Purpose

   Localization: C1 Cloning Vector, Excitation: 485, Emission: 507

   Depositor

   Michael Davidson, Geoffrey Waldo

   Article

   Pédelacq et al Nat Biotechnol. 2006 Jan;24(1):79-88. do

   Type

   Empty backbone

   Use

   Tags

   sfGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pVC-FUS-WT

   Plasmid

   #175066

   Purpose

   expresses mVenus and mCherry tagged to FUS wildtype in mammalian cells

   Depositor

   Arnold Boersma

   Article

   Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018

   Insert

   FUS glycine rich protein (FUS Human)
   

   Use

   Tags

   mVenus + mCherry

   Expression

   Mammalian

   Mutation

   mVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…

   Promoter

   CMV Promoter

   Available since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pShuttle-Flag-XBP1u

   Plasmid

   #63679

   Purpose

   Adenoviral shuttle vector for XBP1u expression

   Depositor

   Qingbo Xu, Lingfang Zeng

   Article

   Zeng et al Proc Natl Acad Sci U S A. 2009 May 19;10

   Insert

   XBP1 (XBP1 Human)
   

   Use

   Adenoviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   unspliced

   Promoter

   CMV

   Available since

   March 23, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pET-pAG-Hia5

   Plasmid

   #174373

   Purpose

   Plasmid for IPTG-inducible expression and purification of protein A/G-Hia5-6xHis (pAG-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)

   Depositor

   Aaron Straight

   Article

   Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi

   Insert

   protein AG - hia5
   

   Use

   Tags

   6xHis and Protein A/G

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRSET-mSA

   Plasmid

   #39860

   Depositor

   Sheldon Park

   Article

   Lim et al Biotechnol Bioeng. 2013 Jan;110(1):57-67

   Insert

   monomeric streptavidin
   

   Use

   Tags

   6xHis and FLAG

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. VSV.G

   Plasmid

   #14888

   Depositor

   Tannishtha Reya

   Article

   Reya et al Nature. 2003 May 22. 423(6938):409-14.

   Insert

   VSV-G Envelope
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. MZB211

    Plasmid

    #228494

    Purpose

    10xHis-SUMO (Smt3) tagged Ssod7-fused Pfu Polymerase with fidelity enhancing mutation (E. coli based protein expression)

    Depositor

    Richard Baker

    Insert

    pol
    

    Use

    Tags

    10xHis-SUMO(Smt3)

    Expression

    Bacterial

    Mutation

    A408S

    Promoter

    T7 Promoter

    Available since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3 Flag-humanTRAF2 (1087)

    Plasmid

    #66931

    Purpose

    Mammalian Expression of TRAF2

    Depositor

    Michael Karin

    Insert

    TRAF2 (TRAF2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. kiCAP-AAV-MyoAAV2A

    Plasmid

    #224440

    Purpose

    Rep/Cap plasmid for the production of MyoAAV 2A, a muscle-tropic AAV capsid in mice.

    Depositor

    Pardis Sabeti

    Article

    Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

    Insert

    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    

    Use

    AAV

    Tags

    Expression

    Mutation

    RGDQTTL insert between amino acids 588 and 589 of…

    Promoter

    p41

    Available since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKM496 (deletions) Zeo

    Plasmid

    #109301

    Purpose

    ORBIT integrating plasmid for deletion of target gene, using zeocin as a selection marker.

    Depositor

    Kenan Murphy

    Article

    Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

    Insert

    Bxb1 attB site
    

    Use

    Orbit integrating plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMSCVpuro-TagRFP657

    Plasmid

    #96939

    Purpose

    Retroviral plasmid containing TagRFP657 for labeling of cells

    Depositor

    Lee Grimes

    Article

    Kleeman et al Cytometry A. 2018 Mar 13. doi: 10.1002/c

    Insert

    Tag Red Fluorescent Protein 657
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. FU-tetO-Gateway

    Plasmid

    #43914

    Depositor

    John Gearhart

    Article

    Addis et al PLoS One. 2011;6(12):e28719. doi: 10.137

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector, doxycycli…

    Tags

    6xHis and V5

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet-ON

    Available since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. ALIX-mNeonGreen

    Plasmid

    #191191

    Purpose

    Full-length ALIX with C-terminal mNeonGreen tag.

    Depositor

    Jin Zhang

    Article

    Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

    Insert

    ALIX (PDCD6IP Synthetic, Human)
    

    Use

    Tags

    6xHIS and mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLVX-NPM1c-IRES-mCherry

    Plasmid

    #131825

    Purpose

    Lentiviral plasmid expressing NPM1 wildtype (cytoplasmic mutant, human) in mammalian cells upon transduction

    Depositor

    Mark Minden

    Article

    Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

    Insert

    NPM1 (NPM1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-enAsCas12a(E174R/S542R/K548R)-NLS(nuc)-3xHA (AAS848)

    Plasmid

    #107941

    Purpose

    Mammalian expression plasmid for human codon optimized enAsCas12a (enhanced AsCas12a) encoding E174R/S542R/K548R substitutions

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized enAsCas12a (E174R/S542R/K548R)
    

    Use

    Tags

    3x HA and NLS (nucleoplasmin)

    Expression

    Mammalian

    Mutation

    E174R, S542R and K548R

    Promoter

    CAG

    Available since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-FLEX-DTR-GFP (AAV2)

    Viral Prep

    #124364-AAV2

    Purpose

    Ready-to-use AAV2 particles produced from pAAV-FLEX-DTR-GFP (#124364). In addition to the viral particles, you will also receive purified pAAV-FLEX-DTR-GFP plasmid DNA. CBA-driven, Cre-dependent expression of DTR-GFP. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Thomas Jessell, Eiman Azim

    Article

    Azim et al Nature. 2014 Apr 17;508(7496):357-63. do

    Promoter

    Chicken B-actin

    Tags

    GFP (Cre-dependent)

    Available since

    June 16, 2020

    Availability

    Academic Institutions and Nonprofits only

20. pEGFP Centrin2 (Nigg UK185)

    Plasmid

    #41147

    Depositor

    Erich Nigg

    Article

    Kleylein-Sohn et al Dev Cell. 2007 Aug;13(2):190-202.

    Insert

    Centrin-2 (CETN2 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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