We narrowed to 4,430 results for: Erf

1. pCN-EF2132tet

   Plasmid

   #107191

   Purpose

   Expresses EF2132 recombinase for performing recombineering in S. aureus

   Depositor

   Steve Salipante

   Article

   Penewit et al MBio. 2018 Feb 20;9(1). pii: mBio.00067-

   Inserts

   EF2132
   tetR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   p23

   Available since

   Feb. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTn7-SCOUT11.G

   Plasmid

   #200999

   Purpose

   pLv1-pUC18R6KT-miniTn7-Gm-sfGFP, GmR, ApR

   Depositor

   Philip Poole

   Article

   Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

   Insert

   superfolder GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23104

   Available since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pM1s3AsG

   Plasmid

   #137921

   Purpose

   E. coli Nissle pMUT1-derived plasmid with ampicillin resistance and constitutive GFP expression

   Depositor

   Neel Joshi

   Article

   Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102

   Insert

   Superfolder GFP
   

   Use

   Synthetic Biology; For use with e. coli nissle in…

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23101

   Available since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pM2s2AsG

   Plasmid

   #137924

   Purpose

   E. coli Nissle pMUT2-derived plasmid with ampicillin resistance and constitutive GFP expression

   Depositor

   Neel Joshi

   Article

   Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102

   Insert

   Superfolder GFP
   

   Use

   Synthetic Biology; For use with e. coli nissle in…

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23101

   Available since

   May 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCRISPR-dCas9

   Plasmid

   #125687

   Purpose

   Gene knockdown for actinomycetes

   Depositor

   Tilmann Weber

   Article

   Tong et al ACS Synth Biol. 2015 Sep 18;4(9):1020-9.

   Insert

   streptomyces codon optimized spdCas9, sgRNA casette
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   ermE*/tipA

   Available since

   Feb. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTn7-SCOUT12.G

   Plasmid

   #201000

   Purpose

   pLv1-pUC18R6KT-miniTn7-Km-sfGFP, KmR, ApR

   Depositor

   Philip Poole

   Article

   Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

   Insert

   superfolder GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23104

   Available since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMT423

   Plasmid

   #139382

   Purpose

   Constitutively expressed dCas9, LacI and TetR, pNBU2 backbone, AmpR, TetR

   Depositor

   Christopher Voigt

   Article

   Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.

   Insert

   Constitutively expressed dCas9, LacI and TetR
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   P1

   Available since

   Dec. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHdzCas9-KRAB

   Plasmid

   #92341

   Purpose

   Expression dzCas9-KRAB in microalgae. CRISPR/Cas based plant genome editing and gene regulation, Hyg resistance

   Depositor

   I-Son Ng

   Article

   Kao et al Bioresour Technol. 2017 May 4. pii: S096

   Type

   Empty backbone

   Use

   CRISPR; Plant expression

   Tags

   KRAB

   Expression

   Plant

   Mutation

   Promoter

   CaMV35S

   Available since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJT119b

   Plasmid

   #50551

   Purpose

   Expresses CcaS and CcaR constitutively, and sfGFP under the PcpcG2 promoter

   Depositor

   Jeffrey Tabor

   Article

   Olson et al Nat Methods. 2014 Mar 9. doi: 10.1038/nm

   Inserts

   CcaS
   CcaR
   sfGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pWS5399-CRISPRai-ZeoR

    Plasmid

    #182838

    Purpose

    Saccharomyces cerevisiae inducible CRISPRai vector - ZeoR marker

    Depositor

    Rodrigo Ledesma-Amaro

    Article

    Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pdCas9-J23109

    Plasmid

    #113151

    Purpose

    Plasmid constitutively expressing dCas9 protein

    Depositor

    Chong Zhang

    Article

    Guo et al Nucleic Acids Res. 2018 Aug 21;46(14):70

    Insert

    dCas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A & H840A

    Promoter

    Available since

    Dec. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pWZL Hygro-NMYC TRF2 deltaB deltaM

    Plasmid

    #16355

    Depositor

    Titia de Lange

    Article

    Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

    Insert

    hTRF2 (TERF2 Human)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Deleted Basic Domain (aa1-44) and Myb Domain (aa4…

    Promoter

    Available since

    May 14, 2008

    Availability

    Academic Institutions and Nonprofits only

13. pHAGE-TO-dCas9-P2A-HSA

    Plasmid

    #121936

    Purpose

    CRISPR-Sirius plasmid

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

    Insert

    dCas9-P2A-HSA
    

    Use

    CRISPR and Lentiviral

    Tags

    HSA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TO

    Available since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEutRwc

    Plasmid

    #216334

    Purpose

    An IPTG-inducible promoter controlling expression of eutR; PEutS, extended controlling expression of sfgfp

    Depositor

    Mark Styczynski

    Article

    McNerney et al ACS Synth Biol. 2020 Feb 21;9(2):402-411

    Inserts

    EutR
    Superfolder green fluorescent protein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 23, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pWS5321-CRISPRai-Markerless

    Plasmid

    #182828

    Purpose

    Saccharomyces cerevisiae inducible CRISPRai vector - markerless

    Depositor

    Rodrigo Ledesma-Amaro

    Article

    Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAbaMCi-BsaI_pJMP2776

    Plasmid

    #208890

    Purpose

    Acinetobacter baumannii CRISPRi plasmid for cloning new guides (BsaI cloning site in gRNA cassette).

    Depositor

    Jason Peters

    Article

    Ward et al mBio. 2023 Dec 21:e0205123. doi: 10.1128

    Insert

    dCas9, sgRNA expression cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSX2720

    Plasmid

    #200764

    Purpose

    Expresses Superfolder GFP-tag fused single change variable fragment antibody of GCN4 (Suntag) with the tissue-specific promoter col-19 in C. elegans.

    Depositor

    Suhong Xu

    Article

    Hu et al Elife. 2023 Mar 3;12:e82178. doi: 10.755

    Insert

    scFv-sfGFP
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    col-19

    Available since

    Aug. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAUX_OL

    Plasmid

    #166246

    Purpose

    use as an auxiliary temperature-sensitive plasmid to successfully transform pdCas9_CL plasmid

    Depositor

    Domitilla Del Vecchio

    Article

    Huang et al Nat Commun. 2021 Mar 16;12(1):1692. doi:

    Insert

    [P112-sgRNA-term]-[J23116_B34-dCas9-B15]
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ec-TTL-P112 and BBa_J23116

    Available since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMM704

    Plasmid

    #68891

    Purpose

    IPTG-inducible CRISPRi vector targeting BT1854, pNBU2 backbone, AmpR

    Depositor

    Christopher Voigt, Timothy Lu

    Article

    Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

    Inserts

    dCas9
    LacI
    sgRNA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pC28-LIC-sfGFP-His

    Plasmid

    #165479

    Purpose

    pET based vector for protein expression in E.coli for targets fused with C-terminal superfolder GFP and His tag

    Depositor

    Renato Bruni

    Article

    Bruni et al Curr Protoc Protein Sci. 2013 Nov 5;74:2

    Type

    Empty backbone

    Use

    Tags

    TEV-GFP-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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