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eIF3k- pMK293 (mAID-mCherry2-Hygro) plasmid

Plasmid

#192244

Purpose

knockin donor vector of mAID-mCherry2-Hygro to C-terminus of endogenous human eIF3k

Depositor

Dieter Wolf

Publication

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

eIF3k-knockin-homology arm-mAID-mCherry2-Hygro (EIF3K Human)

Expression

Mammalian

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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eIF3b- pMK289 (mAID-mClover-NeoR) plasmid

Plasmid

#192237

Purpose

knockin donor vector of mAID-mClover-NeoR to C-terminus of endogenous human eIF3b

Depositor

Dieter Wolf

Publication

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

eIF3b-knockin-homology arm-mAID-mClover-NeoR (EIF3B Human)

Expression

Mammalian

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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eIF3b- pMK293 (mAID-mCherry2-Hygro) plasmid

Plasmid

#192238

Purpose

knockin donor vector of mAID-mCherry2-Hygro to C-terminus of endogenous human eIF3b

Depositor

Dieter Wolf

Publication

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

eIF3b-knockin-homology arm-mAID-mCherry2-Hygro (EIF3B Human)

Expression

Mammalian

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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psiCHECK2-mMOV10-3'UTR-MRE16+MRE153-mut

Plasmid

#178910

Purpose

dual luminescence reporter to assess miRNA responsiveness in the 3'UTR of mouse Mov10 RNA helicase

Depositor

Constance Ciaudo

Publication

Arora et al EMBO Rep. 2022 Jul 20:e54458. doi: 10.15

Insert

3'UTR mMov10 (Mov10 Mouse)

Use

Luciferase

Mutation

CTG>AGT and TGC>CAT mir16+153 mutated

Promoter

SV40 promoter

Available since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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psiCHECK2-mMOV10-3'UTR-MRE153-mut4

Plasmid

#178909

Purpose

dual luminescence reporter to assess miRNA responsiveness in the 3'UTR of mouse Mov10 RNA helicase

Depositor

Constance Ciaudo

Publication

Arora et al EMBO Rep. 2022 Jul 20:e54458. doi: 10.15

Insert

3'UTR mMov10 (Mov10 Mouse)

Use

Luciferase

Mutation

TGC>CAT miR153-3p MRE mutated

Promoter

SV40 promoter

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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FH95pUP95S73AGW (F2)

Plasmid

#74039

Purpose

lentiviral expression of Psd95 shRNA and Psd95-S73A-EGFP fusion

Depositor

Robert Malenka, Bernardo Sabatini, Weifeng Xu

Publication

Steiner et al Neuron. 2008 Dec 10;60(5):788-802. doi:

Insert

Psd95

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

S73A

Promoter

H1

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only
FH95pUP95Q15AD45_64GW (D8)

Plasmid

#74031

Purpose

lentiviral expression of Psd95 shRNA and Psd95-Q15A _45_64-EGFP fusion

Depositor

Robert Malenka, Oliver Schluter, Weifeng Xu

Publication

Xu et al Neuron. 2008 Jan 24;57(2):248-62. doi: 1

Insert

Psd95

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

Q15A, D45_64

Promoter

H1

Available since

July 26, 2019

Availability

Academic Institutions and Nonprofits only
FH95pUP95L460pGW (E2)

Plasmid

#74032

Purpose

lentiviral expression of Psd95 shRNA and Psd95-L460P-EGFP fusion

Depositor

Robert Malenka, Oliver Schluter, Weifeng Xu

Publication

Xu et al Neuron. 2008 Jan 24;57(2):248-62. doi: 1

Insert

Psd95

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

L460P

Promoter

H1

Available since

July 26, 2019

Availability

Academic Institutions and Nonprofits only
FH95pUP95DSKIrGW (D2)

Plasmid

#74025

Purpose

lentiviral expression of Psd95 shRNA and Psd95dSK and EGFP

Depositor

Robert Malenka, Oliver Schluter, Weifeng Xu

Publication

Xu et al Neuron. 2008 Jan 24;57(2):248-62. doi: 1

Insert

Psd95

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

delta SK

Promoter

H1

Available since

July 26, 2019

Availability

Academic Institutions and Nonprofits only
FH95pUG+p97W (B8)

Plasmid

#74017

Purpose

lentiviral expression of Psd95 shRNA, and EGFP-SAP97beta fusion

Depositor

Robert Malenka, Oliver Schluter, Weifeng Xu

Publication

Schluter et al Neuron. 2006 Jul 6;51(1):99-111.

Insert

Psd95 shRNA

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

H1

Available since

Jan. 5, 2018

Availability

Academic Institutions and Nonprofits only

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RSV/mGATA3 (antisense)

Plasmid

#83817

Purpose

contains antisense mouse GATA3

Depositor

Douglas Engel

Publication

Ko et al Mol Cell Biol. 1991 May . 11(5):2778-84.

Insert

GATA3-antisense strand (Gata3 Mouse)

Expression

Mammalian

Promoter

RSV

Available since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only
pBP-TL6

Plasmid

#72990

Purpose

RBS_M3

Depositor

Paul Freemont

Publication

Moore et al ACS Synth Biol. 2016 May 2.

Insert

M3 RBS

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only
pBP-TL7

Plasmid

#72991

Purpose

RBS_M5

Depositor

Paul Freemont

Publication

Moore et al ACS Synth Biol. 2016 May 2.

Insert

M5 RBS

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only
pBP-TL9

Plasmid

#72993

Purpose

RBS_L2

Depositor

Paul Freemont

Publication

Moore et al ACS Synth Biol. 2016 May 2.

Insert

L2 RBS

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pBP-TL10

Plasmid

#72994

Purpose

RBS_L3

Depositor

Paul Freemont

Publication

Moore et al ACS Synth Biol. 2016 May 2.

Insert

L3 RBS

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only
pBP-TL12

Plasmid

#72996

Purpose

RBS_L5

Depositor

Paul Freemont

Publication

Moore et al ACS Synth Biol. 2016 May 2.

Insert

L5 RBS

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only
pBP-BBa_B0012

Plasmid

#72997

Purpose

BBa_B0012 iGEM terminator (5' TCGA/ 3' TGTT fusion)

Depositor

Paul Freemont

Publication

Moore et al ACS Synth Biol. 2016 May 2.

Insert

Bba_B0012 terminator

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pBP-L3S2P21

Plasmid

#72999

Purpose

L3S2P21 terminator

Depositor

Paul Freemont

Publication

Moore et al ACS Synth Biol. 2016 May 2.

Insert

L3S2P21 terminator

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pBP-L3S1P51

Plasmid

#73000

Purpose

L3S1P51 terminator

Depositor

Paul Freemont

Publication

Moore et al ACS Synth Biol. 2016 May 2.

Insert

L3S1P51 terminator

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pBP-L3S1P32

Plasmid

#73001

Purpose

L3S1P32 terminator

Depositor

Paul Freemont

Publication

Moore et al ACS Synth Biol. 2016 May 2.

Insert

L3S1P32 terminator

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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