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Plasmid#60551PurposeExpresses CibN-dCas9 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCibN-dCas9
UseCRISPRTagsFLAG and SV40 NLSExpressionMammalianMutationinactivating Cas9 mutations D10A and H840APromoterCMVAvailable sinceFeb. 2, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGTVL2-CRBN-TBD
Plasmid#196369Purposeprotein expression of the GST-tagged Cereblon Thalidomide binding domainDepositorInsertCRBN (CRBN Human)
TagsHis6-GST-TEVExpressionBacterialMutationcontains only T319-T425PromoterT7Available sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
LiOn-CMV∞Cre
Plasmid#154018PurposeVector based on the LiOn integration-coupled translational switch (Kumamoto et al bioRxiv 2019) expressing a functional Cre recombinase from a CMV promoter upon action of the piggyBac transposaseDepositorInsertCre-FLAG
ExpressionMammalianPromoterCMVAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pScI_dCas9-CDA
Plasmid#108549PurposeBacterial Target-AID vectorDepositorInsertdCas9-PmCDA1
TagsFlagExpressionBacterialMutationD10A and H840A for SpCas9Promoterlambda ORAvailable sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUDP004
Plasmid#101165PurposeE. coli/S. cerevisiae shuttle vector carrying amd S marker and Spcas9D147Y P411T allowing cloning of ribozyme flanked g-RNA for Cas9 editing (HH-gRNA-HDV)DepositorTypeEmpty backboneUseCRISPRExpressionYeastPromoterScTDH3Available sinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pmAmetrine1.2
Plasmid#42171DepositorInsertmAmetrine1.2
Tags6HisExpressionBacterialPromoteraraBADAvailable sinceFeb. 6, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBHM2329
Plasmid#171686PurposepRS316-PCnU6-scaffold, contains template for amplification of PCnU6 and scaffold for fusion PCRDepositorInsertPCnU6-sgRNA_scaffold
UseCRISPRExpressionBacterial and YeastPromoterC. neoformans U6Available sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-EFS-DIO-GRAB_Ado1.0
Plasmid#153291PurposeExpress the adenosine sensor GRAB_Ado1.0 in Cre positive cellsDepositorInsertAdenosine sensor GRAB_Ado1.0
UseAAVPromoterEFSAvailable sinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lenti-CamKII-PSD95.FingR-eGFP-CCR5TC
Plasmid#126218PurposeGlobal labeling of excitatory synapses (green fluorescence)DepositorInsertPSD95.FingR-eGFP-CCR5TC
UseLentiviralExpressionMammalianPromoterCamKIIaAvailable sinceJuly 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
EF1a_ETV2_P2A_Hygro_Barcode
Plasmid#120436PurposeBarcoded lentiviral vector to express ETV2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorAvailable sinceApril 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
tdTomato-VE-Cadherin-N-10
Plasmid#58142PurposeLocalization: Tight Junctions, Excitation: 554, Emission: 581DepositorInsertVE-Cadherin (CDH5 Human)
TagstdTomatoExpressionMammalianMutationI503T in VE-CadherinPromoterCMVAvailable sinceNov. 19, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pThermoCas9i_ctrl
Plasmid#100982PurposeExpresses the catalytically inactive version of ThermoCas9 (Thermo(d)Cas9) and its sgRNA moduleDepositorInsertsCatalytically inactive variant of the Cas9 from the type IIc CRISPR-Cas sytem of Geobacillus thermodenitrificans T12 strain
ThermoCas9 single guide RNA expressing module
TagsNoneExpressionBacterialMutationchanged aspartate 8 to alanine and histidine 582 …PromoterB. coagulans DSM 1 pta promoter and B. smithii xy…Available sinceNov. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLV-mTurquoise-MLC-IRES-neo
Plasmid#85145Purposelentiviral expression of myosin light chain (MLC, MYL9), N-terminally tagged with mTurquoiseDepositorInsertmTurquoise-MLC (myosin regulatory light chain, N-terminally tagged with mTurquoise (MYL9 Human)
UseLentiviralTagsmTurquoisePromoterEF1aAvailable sinceFeb. 13, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCEP4-puroeGFP
Plasmid#177922PurposeConstitutively expresses eGFP with puromycin selectionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserteGFP
ExpressionMammalianPromoterCMVAvailable sinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET15b T7pCONS TIR-2 sfGFP
Plasmid#154468PurposepET15b with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFPDepositorInsertsuper folder GFP
TagsHis-thrombinExpressionBacterialPromoterT7Available sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-synapsin-HaloCaMP1b-EGFP
Plasmid#138328Purposechemigenetic calcium indicatorDepositorHas serviceAAV1 and AAV9InsertHaloCaMP1b
UseAAVTagsEGFP, His-tag, and Nuclear Exclusion SignalExpressionMammalianPromotersynapsinAvailable sinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCFJ150-mCherry(dpiRNA)::ANI-1(AHPH)
Plasmid#107939Purposepie-1p::mCherry(dpiRNA)::ANI-1(AHPH)::pie-1 3'UTR construct used for MosSCI in nematode. mCherry is optimized by removing all piRNA targeting sites to allow germline expression. RhoA biosenser.DepositorInsertmCherry(dpiRNA)::ANI-1(AHPH)
ExpressionWormPromoterpie-1Available sinceApril 2, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
act-AsCpf1
Plasmid#73917Purposeexpression plasmid of AsCpf1DepositorInserthAsCpf1
TagsNLS-3xHAExpressionInsectPromoteract5CAvailable sinceMarch 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pABE8e-NG-403Q-Nterm-AAV
Plasmid#194651PurposeN-terminal AAV genome plasmid encoding ABE8e + sgRNA to correct the R403Q mutation in miceDepositorInsertABE8e-NG N-term
UseAAVTagsBPNLS and NpuN spilt inteinPromoterTNNT2Available sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDonor14-ShHELIX-KanR (CJT70)
Plasmid#181793PurposepDonor for ShHELIX containing 14bp of spacing between the I-AniI site and LE/RE and encoding a kanamycin resistance gene as cargoDepositorInsertKanR, R6K origin of replication
ExpressionBacterialAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only