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pD441-H6-SUMO-HyPerFLEX

Plasmid

#216676

Purpose

Plasmid with cleavable His-tag for expression of the HyPer-FLEX sensor for hydrogen peroxide in E. coli

Depositor

Joris Messens

Article

Potekhina et al Nat Chem Biol. 2025 Oct 16. doi: 10.1038

Insert

HyPerFLEX

Tags

6xHis-SUMO

Expression

Bacterial

Promoter

T5

Available Since

Oct. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN-DEST (JDW 471)

Plasmid

#242573

Purpose

Gateway destination vector with a CAGGS promoter in the backbone.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Use

Gateway subcloning

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pYTRW10K_0x5

Plasmid

#177278

Purpose

genomic integration of genes (inserted in I-SceI-site) via transposon Tn5

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Type

Empty backbone

Use

Synthetic Biology; Yeast expression, tn5 genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pET21a asyn(66-140) E83A

Plasmid

#240583

Purpose

Overexpress truncated alpha-Synuclein protein

Depositor

Jennifer Lee

Article

McGlinchey et al Nat Commun. 2025 Apr 23;16(1):3825. doi:

Insert

aSyn (SNCA Human)

Tags

6xHis

Expression

Bacterial

Mutation

aa 66-140 only, E83A

Promoter

T7

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pET21a asyn(66-140) K80A

Plasmid

#240584

Purpose

Overexpress truncated alpha-Synuclein protein

Depositor

Jennifer Lee

Article

McGlinchey et al Nat Commun. 2025 Apr 23;16(1):3825. doi:

Insert

aSyn (SNCA Human)

Tags

6xHis

Expression

Bacterial

Mutation

aa 66-140 only, K80A

Promoter

T7

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pT7-7 asyn K97A

Plasmid

#240601

Purpose

Overexpress mutant alpha-Synuclein protein

Depositor

Jennifer Lee

Article

McGlinchey et al Nat Commun. 2025 Apr 23;16(1):3825. doi:

Insert

aSyn (SNCA Human)

Expression

Bacterial

Mutation

K97A

Promoter

T7

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pET21a asyn(66-140) K96A

Plasmid

#240585

Purpose

Overexpress truncated alpha-Synuclein protein

Depositor

Jennifer Lee

Article

McGlinchey et al Nat Commun. 2025 Apr 23;16(1):3825. doi:

Insert

aSyn (SNCA Human)

Tags

6xHis

Expression

Bacterial

Mutation

aa 66-140 only, K96A

Promoter

T7

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pT7-7 asyn K96A

Plasmid

#240600

Purpose

Overexpress mutant alpha-Synuclein protein

Depositor

Jennifer Lee

Article

McGlinchey et al Nat Commun. 2025 Apr 23;16(1):3825. doi:

Insert

aSyn (SNCA Human)

Expression

Bacterial

Mutation

K96A

Promoter

T7

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV_CaMKII-RFP-p2A-DAAO-NES

Plasmid

#238918

Purpose

Mammalian expression of DAAO with a nuclear export signal and RFP as a reporter in excitatory neurons

Depositor

Vsevolod Belousov

Article

Kalinichenko et al Redox Biol. 2023 Apr;60:102604. doi: 10.

Insert

RFP-p2A-DAAO-NES

Use

AAV

Expression

Mammalian

Promoter

CaMKIIalfa

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV_CMV-RFP-p2A-DAAO-NES

Plasmid

#238920

Purpose

Expression of DAAO with a nuclear export signal and RFP as a reporter in mammalian cells

Depositor

Vsevolod Belousov

Article

Kalinichenko et al Redox Biol. 2023 Apr;60:102604. doi: 10.

Insert

RFP-p2A-DAAO-NES

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pET21a asyn(66-140) K97A

Plasmid

#240586

Purpose

Overexpress truncated alpha-Synuclein protein

Depositor

Jennifer Lee

Article

McGlinchey et al Nat Commun. 2025 Apr 23;16(1):3825. doi:

Insert

aSyn (SNCA Human)

Tags

6xHis

Expression

Bacterial

Mutation

aa 66-140 only, K97A

Promoter

T7

Available Since

Aug. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pET21a asyn(66-140) K102A

Plasmid

#240588

Purpose

Overexpress truncated alpha-Synuclein protein

Depositor

Jennifer Lee

Article

McGlinchey et al Nat Commun. 2025 Apr 23;16(1):3825. doi:

Insert

aSyn (SNCA Human)

Tags

6xHis

Expression

Bacterial

Mutation

aa 66-140 only, K102A

Promoter

T7

Available Since

Aug. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pET21a asyn(66-140) D98A

Plasmid

#240587

Purpose

Overexpress truncated alpha-Synuclein protein

Depositor

Jennifer Lee

Article

McGlinchey et al Nat Commun. 2025 Apr 23;16(1):3825. doi:

Insert

aSyn (SNCA Human)

Tags

6xHis

Expression

Bacterial

Mutation

aa 66-140 only, D98A

Promoter

T7

Available Since

Aug. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pT7-7 asyn K80A

Plasmid

#240599

Purpose

Overexpress mutant alpha-Synuclein protein

Depositor

Jennifer Lee

Article

McGlinchey et al Nat Commun. 2025 Apr 23;16(1):3825. doi:

Insert

aSyn (SNCA Human)

Expression

Bacterial

Mutation

K80A

Promoter

T7

Available Since

Aug. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE Flag HA ADAR2

Plasmid

#237856

Purpose

Lentiviral expression of ADAR2

Depositor

David Pellman

Article

Tang et al Nature. 2022 Jun;606(7916):930-936. doi:

Insert

ADAR2 (ADARB1 Human)

Expression

Mammalian

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pLIX401 C-term SETX

Plasmid

#237999

Purpose

Inducible lentiviral expression of C-term SETX

Depositor

David Pellman

Article

Tang et al Nature. 2022 Jun;606(7916):930-936. doi:

Insert

SETX (SETX Human)

Use

Lentiviral

Expression

Mammalian

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pB-DEST (JDW 1205)

Plasmid

#229821

Purpose

A PiggyBac destination vector compatible with gateway cloning system.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pB-Neo-DEST (JDW 940)

Plasmid

#229825

Purpose

A neo selectable, PiggyBac destination vector compatible with gateway cloning system.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2-phiC31o (JDW 453)

Plasmid

#229842

Purpose

A CMV-driven Phi31 recombinase.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

phiC31 codon optimized, PhiC31o

Expression

Mammalian

Available Since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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8_T7-PH-mScarlet3

Plasmid

#225935

Purpose

In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the PH membrane localisation signal

Depositor

Michalis Averof

Article

Karapidaki et al bioRxiv 2024.11.12.623055

Insert

PH-mScarlet3

Use

In vitro transcription

Promoter

T7

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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