Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 9,684 results for: CAG

Clear keywords
Showing: 3301 - 3320 of 9684 results

  1. pAAV-CMV-SpCas9D10A nickase

    Plasmid
    #216737
    Purpose
    Expresses SpCas9-D10A nickase from a CMVd1 promoter. For AAV packaging. Derived from pAAV-CMV-SpCas9 (Addgene #113034)
    Depositor
    Vincent Dion
    Article
    Alvaro et al bioRxiv
    Insert
    Cas9-D10A nickase
    Use
    AAV and CRISPR
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    CMVd1
    Available Since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    216737-plasmid-map-sequence-id-430337

    Enlarge

  2. pAAV-U6-sgCTG-CMV-GFP

    Plasmid
    #216734
    Purpose
    AAV plasmid containing sgCTG (target sequence: (CUG)6) driven by a U6 promoter and eGFP under the control of the CMV promoter. Used for in vivo experiments.
    Depositor
    Vincent Dion
    Article
    Alvaro et al bioRxiv
    Insert
    sgCTG
    Use
    AAV and CRISPR
    Expression
    Mammalian
    Promoter
    U6 and CMV
    Available Since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    216734-plasmid-map-sequence-id-430319

    Enlarge

  3. pAAV-nEF-SpCas9D10A nickase

    Plasmid
    #216736
    Purpose
    Expresses SpCas9-D10A nickase from a nEF promoter. For AAV packaging. Derived from pAAV-nEF-SpCas9 (Addgene #87115)
    Depositor
    Vincent Dion
    Article
    Alvaro et al bioRxiv
    Insert
    Cas9-D10A nickase
    Use
    AAV and CRISPR
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    EF-1-alpha core
    Available Since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    216736-plasmid-map-sequence-id-430323

    Enlarge

  4. pSLQ10873

    Plasmid
    #183961
    Purpose
    U6-SKSKSO(crRNA array)-CAG-hyperdCas12a-HA-miniVPR
    Depositor
    Stanley Qi
    Article
    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d
    Inserts
    HyperdCas12a
    poly-crRNA array targeting Sox2-Klf4-Oct4
    Use
    CRISPR
    Tags
    HA
    Expression
    Mammalian
    Mutation
    D832A, D156R, E292R, D235R, D350R
    Promoter
    CAG and U6
    Available Since
    Aug. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    183961-plasmid-map-sequence-id-369669

    Enlarge

  5. pSLQ10878

    Plasmid
    #183964
    Purpose
    CAG-hyperCas12a(nuclease active)-HA-P2A-mCherry-T2A-HygroR
    Depositor
    Stanley Qi
    Article
    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d
    Insert
    HyperdCas12a
    Use
    CRISPR
    Tags
    HA, Hygromycin Resistance, and mCherry
    Expression
    Mammalian
    Mutation
    D156R, E292R, D235R, D350R
    Promoter
    CAG
    Available Since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    183964-plasmid-map-sequence-id-363120

    Enlarge

  6. miCBE

    Plasmid
    #205413
    Purpose
    Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with APOBEC3A and UGI driven by CAG promoter, optimized _RNA (_RNA*) driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Yingsi Zhou
    Article
    Han et al Nat Methods. 2023 May 25. doi: 10.1038/s
    Insert
    pU6__RNA*_pCAG_bpNLS_APOBEC3A(W104A)_XTEN_OgeuIscB*(D61A)_NLS_2xUGI_bGHployA_pCMV_mCherry
    Expression
    Mammalian
    Mutation
    D61A+E85R+H369R+S387R+S457R
    Promoter
    hU6, CAG, CMV
    Available Since
    Dec. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    205413-plasmid-map-sequence-id-416749

    Enlarge

  10. pLV(gRNA)-CMV-eGFP-U6(sgCTG)

    Plasmid
    #216732
    Purpose
    Contains a eGFP gene along with a U6 promoter driving the sgCTG (target sequence: (CUG)6). Used with the HD iPSC-derived astrocytes
    Depositor
    Vincent Dion
    Article
    Alvaro et al bioRxiv
    Insert
    sgCTG lentiviral guide RNA with eGFP tag
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    CMV and U6
    Available Since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    216732-plasmid-map-sequence-id-430316

    Enlarge

  12. PB-TRE3G-dN40-TP53-CCRB

    Plasmid
    #236774
    Purpose
    A piggybac-based cloning vector containing TRE3G promoter-driven short-form of TP53 and CAG promoter-driven nuclear-localized Clover-P2A-rtTA-IRES-BSD.
    Depositor
    Kazutoshi Takahashi
    Article
    Okubo et al Sci Adv. 2025 Apr 11;11(15):eadq5484. do
    Insert
    TP53 (TP53 Human)
    Use
    Piggybac
    Expression
    Mammalian
    Mutation
    deleted amino acids 1-40
    Promoter
    TRE3G promoter and TRE3G promoter, CAG promoter
    Available Since
    May 29, 2025
    Availability
    Academic Institutions and Nonprofits only
    236774-plasmid-map-sequence-id-473566

    Enlarge

  14. TRE-FRT-MCS-WPRE-SV40 - GCH27

    Plasmid
    #230828
    Purpose
    AAV cloning vector for Flpo-dependent expression under the TRE promoter.
    Depositor
    Kimberly Ritola
    Type
    Empty backbone
    Use
    AAV; Cloning vector, flp/frt
    Available Since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    230828-plasmid-map-sequence-id-466645

    Enlarge

  15. IGI-P0492 pHR-dCas9-NLS-VPR-mCherry

    Plasmid
    #102245
    Purpose
    Lentiviral expression of dCas9-VPR-mCherry fusion protein for CRISPRa.
    Depositor
    Jacob Corn
    Insert
    dCas9-VPR-mCherry
    Use
    CRISPR and Lentiviral
    Tags
    NLS-VPR-mCherry
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Oct. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    102245-plasmid-map-sequence-id-354473

    Enlarge

  16. pmCellFree_KA1_SARS-COV-2_NSP3

    Plasmid
    #169378
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP3 with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _NSP3
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Promoter
    T7- CMV/CAG
    Available Since
    June 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    169378-plasmid-map-sequence-id-335064

    Enlarge

  17. pmCellFree_KA1_SARS-COV-2_NSP2

    Plasmid
    #169377
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP2 with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _NSP2
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Promoter
    T7- CMV/CAG
    Available Since
    June 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    169377-plasmid-map-sequence-id-335058

    Enlarge

  18. pmCellFree_KA1_SARS-COV-2_ORF9b

    Plasmid
    #169396
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus ORF9bwith N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _ORF9b
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Promoter
    T7- CMV/CAG
    Available Since
    June 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    169396-plasmid-map-sequence-id-335426

    Enlarge

  19. pmCellFree_KA1_SARS-COV-2_NSP9

    Plasmid
    #169383
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP9 with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _NSP9
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Promoter
    T7- CMV/CAG
    Available Since
    July 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    169383-plasmid-map-sequence-id-336869

    Enlarge

  20. pmCellFree_KA1_SARS-COV-2_NSP12

    Plasmid
    #169385
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP12 with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _NSP12
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Promoter
    T7- CMV/CAG
    Available Since
    July 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    169385-plasmid-map-sequence-id-336500

    Enlarge

Showing: 3301 - 3320 of 9684 results