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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 66,906 results for: nin

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Showing: 3301 - 3320 of 66906 results

  1. pCMV-T7-ABE8e-nSpG-P2A-EGFP (KAC984)

    Plasmid
    #185911
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized ABE8e-nSpG-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpG=D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T133…
    Promoter
    CMV and T7
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    185911-plasmid-map-sequence-id-367364

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  2. pTwist-SARS-CoV-2 Δ18 B.1.617.2v1

    Plasmid
    #179905
    Purpose
    Encodes SARS-CoV-2 B.1.617.2v1 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production
    Depositor
    Alejandro Balazs
    Publication
    Garcia-Beltran et al Cell. 2022 Jan 6. pii: S0092-8674(21)014
    Insert
    SARS-CoV-2 B.1.617.2v1 Spike truncated to remove 18 amino acids (S Severe acute respiratory syndrome coronavirus 2)
    Expression
    Mammalian
    Mutation
    T19R; G142D; E156G; Δ157-158; L452R; T478K; D614G…
    Promoter
    CAG
    Available since
    Jan. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    179905-plasmid-map-sequence-id-351283

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  3. G9A[SET]-dCas9

    Plasmid
    #100089
    Purpose
    Catalytic domain [SET] of human G9A fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression
    Depositor
    David Segal
    Publication
    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    G9A (EHMT2 Human)
    Use
    CRISPR
    Tags
    3XFLag-NLS-G9A[SET]-dCas9-NLS
    Expression
    Mammalian
    Mutation
    G9A[SET] includes aa 829-1209
    Promoter
    CMV
    Available since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    100089-plasmid-map-sequence-id-197561

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  4. pNTI194

    Plasmid
    #84266
    Purpose
    Expresses Avi-tagged RPL1 and humanized birA
    Depositor
    Nicholas Ingolia
    Publication
    Ingolia et al Cell Rep. 2014 Sep 11;8(5):1365-79. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    RPL1 (RPL10A Human, (*see below))
    humanized birA
    Tags
    Avi tag, Flag, Gly/Ser linker, and TEV site
    Expression
    Mammalian
    Mutation
    R33A, A34E (*see below)
    Promoter
    CMV and P2A peptide
    Available since
    Oct. 31, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    84266-plasmid-map-sequence-id-160813

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  5. delta DE OCT4-GFP-2A-PURO transgenic reporter

    Plasmid
    #52381
    Purpose
    BAC recombineering generated construct, used as a reporter for OCT4 expression
    Depositor
    Jacob Hanna
    Publication
    Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    GFP-2A-puro
    OCT4 (POU5F1 Human)
    Expression
    Mammalian
    Mutation
    deleted -3194 - -1768 upstream from OCT4 ATG
    Promoter
    OCT4
    Available since
    June 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    52381-plasmid-map-sequence-id-82024

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  10. CMVp-dsRed2-Triplex-28-gRNA1-28-pA

    Plasmid
    #55200
    Purpose
    Plasmid encoding the Triplex/gRNA architecture.This is a modified form of the original plasmid described in the paper (Construct 3). mKate2 was replaced with dsRed2 because of distribution issues.
    Depositor
    Timothy Lu
    Publication
    Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dsRed2
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    55200-plasmid-map-sequence-id-85587

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  17. pET-MCN-10His-mStayGold (E138D)

    Plasmid
    #211361
    Purpose
    Bacterial expression of the bright and photostable monomeric StayGold fluorescent protein (E138D). Codon optimised for expression in Escherichia coli. Contains a N-terminal 10xHis tag.
    Depositor
    Mohan Balasubramanian
    Publication
    Ivorra-Molla et al Nat Biotechnol. 2023 Dec 11. doi: 10.103
    Insert
    mStayGold
    Tags
    10xHis-tag
    Expression
    Bacterial
    Mutation
    E138D
    Promoter
    T7
    Available since
    Dec. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    211361-plasmid-map-sequence-id-417678

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  19. pOPINE-3C-eGFP

    Plasmid
    #41125
    Depositor
    Ray Owens
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GFP
    Expression
    Bacterial, Insect, and Mamm…
    Available since
    April 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    41125-plasmid-map-sequence-id-54698

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  20. pFC902

    Plasmid
    #106904
    Purpose
    The vector has a U3 promoter and terminator controlled gene encoding a transcript containing the sgRNA flanked by a tRNA-Gly repeat; and a gene encoding cas9 controlled by Ptef1 and Ttef1
    Depositor
    Uffe Mortensen
    Publication
    Nodvig et al Fungal Genet Biol. 2018 Jan 8. pii: S108
    Inserts
    cas9
    pyrG
    U3 promoter and terminator controlled gene encoding a transcript containing the sgRNA flanked by a tRNA gly repeat
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Available since
    May 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    106904-plasmid-map-sequence-id-206325

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Showing: 3301 - 3320 of 66906 results