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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,310 results for: LEA

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Showing: 3321 - 3340 of 12310 results

  5. pGBW-m4136461

    Plasmid
    #149538
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    6xHIS;Linker_GGSG;MBP;Linker_GGSG;Cleavable TEV
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149538-plasmid-map-sequence-id-307561

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  10. VCA

    Plasmid
    #27792
    Depositor
    Steven Vogel
    Publication
    Koushik et al PLoS One. 2009 Nov 25;4(11):e8031. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Venus-Cerulean-Amber
    Use
    Stoichiometry standard
    Expression
    Mammalian
    Available since
    June 23, 2011
    Availability
    Academic Institutions and Nonprofits only

  11. pX330-Flag-dSpCas9

    Plasmid
    #92113
    Purpose
    Expression plasmid for human codon-optimized dead/inactive SpCas9 (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dead/inactive SpCas9 with FLAG tag
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    Cbh
    Available since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    92113-plasmid-map-sequence-id-197502

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  12. P100 (3OMC)

    Plasmid
    #28159
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SND1 (SND1 Human)
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    Tudor domain (residues 650-910).
    Available since
    Feb. 28, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  15. pET tdTomato LIC cloning vector (H6-tdTomato)

    Plasmid
    #86928
    Purpose
    pET LIC cloning vector for Biotin-His6-tev-yORF-tdTomato
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Tags
    6xHis, AviTag (biotin), TEV cleavage site, and td…
    Expression
    Bacterial
    Promoter
    T7
    Available since
    April 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    86928-plasmid-map-sequence-id-175058

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  18. pNLS-2xBFP(4C)

    Plasmid
    #176151
    Purpose
    Encodes NLS-2xBFP(4C), which is a tandem BFP with an N-terminal SV40 NLS. The protein has 4 solvent accessible cysteines for labeling with maleimide dyes.
    Depositor
    Siegfried Musser
    Publication
    Chowdhury et al Nat Cell Biol. 2022 Jan;24(1):112-122. d
    Insert
    NLS-2xBFP(4C)
    Tags
    6xHis tag and SV40 nuclear localization sequence …
    Expression
    Bacterial
    Mutation
    Compared with EGFP, the two BFP domains have the …
    Promoter
    Ptrc
    Available since
    March 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    176151-plasmid-map-sequence-id-356068

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Showing: 3321 - 3340 of 12310 results