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pCEP4-tat

Plasmid

#22502

Depositor

Sergey Kasparov

Article

Coleman et al Physiol Genomics. 2003 Feb 6. 12(3):221-

Insert

Transactivator of Transcription

Expression

Mammalian

Available Since

Nov. 24, 2009

Availability

Academic Institutions and Nonprofits only
pHEF-VSVG

Plasmid

#22501

Depositor

Sergey Kasparov

Article

Coleman et al Physiol Genomics. 2003 Feb 6. 12(3):221-

Insert

Vesicular Stomatitis Virus Glycoprotein

Expression

Mammalian

Available Since

March 12, 2010

Availability

Academic Institutions and Nonprofits only
pNHP

Plasmid

#22500

Depositor

Sergey Kasparov

Article

Coleman et al Physiol Genomics. 2003 Feb 6. 12(3):221-

Insert

gag, pol, tat, rev

Expression

Mammalian

Available Since

March 12, 2010

Availability

Academic Institutions and Nonprofits only
CyclinB-GFP

Plasmid

#54465

Purpose

CyclinB-GFP in pIVT vector for in vitro transcription

Depositor

Michael Lampson

Article

Davydenko et al J Cell Biol. 2013 Jul 22;202(2):221-9. d

Insert

CyclinB

Tags

GFP

Expression

Mammalian

Mutation

K67E and I150T mutations compared to GenBank refe…

Available Since

Jan. 13, 2016

Availability

Academic Institutions and Nonprofits only
-24lacZ

Plasmid

#25422

Depositor

David J. Goldhamer

Article

Chen et al Dev Dyn. 2001 Jul . 221(3):274-88.

Insert

Myo D 5' flanking sequence (MYOD1 Human)

Use

Reporter construct

Tags

lacZ

Expression

Mammalian

Available Since

June 29, 2010

Availability

Academic Institutions and Nonprofits only
EC64422

Plasmid

#191768

Purpose

CRISPR mutagenesis in Medicago truncatula

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:221-252. doi

Insert

SpCas9

Use

CRISPR

Expression

Plant

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pHES262 [prADH1-Msn2-mCherry, pnh604]

Plasmid

#103844

Purpose

Yeast Expression Plasmid

Depositor

Hana El-Samad

Article

Stewart-Ornstein et al Mol Biol Cell. 2017 Jan 1;28(1):221-227.

Insert

pAdh1-Msn2-mCherry

Tags

mCherry

Expression

Yeast

Available Since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

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ESCRT-II

Plasmid

#17633

Depositor

James Hurley

Article

Hierro et al Nature. 2004 Sep 9. 431(7005):221-5.

Insert

pST39-Vps25(1-203) - His/Vps22(1-234) - Vps36 (1-567) (VPS36, SNF8, VPS25 Budding Yeast)

Expression

Bacterial

Available Since

March 28, 2008

Availability

Academic Institutions and Nonprofits only

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hVEGF165-eGFP-hVEGF165 glycosylation sequon-His tag

Plasmid

#240484

Purpose

Express engineered glycosylated eGFP in CHO cells

Depositor

Xi Ren

Article

Xing et al Acta Biomater. 2025 Nov;207:221-232. doi

Insert

glycosylated eGFP

Tags

3 repeats of hVEGF165 glycosylation sequon, 6xHis…

Expression

Mammalian

Available Since

Nov. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pFB-MBD2.5R (pc2395)

Plasmid

#211727

Purpose

Expresses MBD2 (aa153-221) RFP tagged in insect cells

Depositor

Cristina Cardoso

Article

Becker et al PLoS One. 2013;8(1):e53730. doi: 10.1371

Insert

MBD2.5 aa153-221

Tags

RFP

Expression

Insect

Promoter

Polyhedrin promotor

Available Since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pHES262 [prADH1-Msn2-mCherry, pnh604]

Plasmid

#103844

Purpose

Yeast Expression Plasmid

Depositor

Hana El-Samad

Article

Stewart-Ornstein et al Mol Biol Cell. 2017 Jan 1;28(1):221-227.

Insert

pAdh1-Msn2-mCherry

Tags

mCherry

Expression

Yeast

Available Since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

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ESCRT-II

Plasmid

#17633

Depositor

James Hurley

Article

Hierro et al Nature. 2004 Sep 9. 431(7005):221-5.

Insert

pST39-Vps25(1-203) - His/Vps22(1-234) - Vps36 (1-567) (VPS36, SNF8, VPS25 Budding Yeast)

Expression

Bacterial

Available Since

March 28, 2008

Availability

Academic Institutions and Nonprofits only

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GFP-C1-AKT-PH

Plasmid

#21218

Depositor

Tobias Meyer

Article

Raucher et al Cell. 2000 Jan 21. 100(2):221-8.

Insert

AKT-PH

Tags

GFP

Expression

Mammalian

Mutation

PH domain of AKT. (Lab plasmid WC 0002).

Available Since

July 10, 2009

Availability

Academic Institutions and Nonprofits only

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pHES609 [prNOp7-PBAC]

Plasmid

#103017

Purpose

A S. cerevisiae plasmid. A photosensitive adenylate cyclase that produces cAMP in response to blue light. Used to activate PKA.

Depositor

Hana El-Samad

Article

Stewart-Ornstein et al Mol Biol Cell. 2017 Jan 1;28(1):221-227.

Insert

bPAC

Expression

Yeast

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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pAM71

Plasmid

#227621

Purpose

pETDuet-1 H6-precission-Rpn11(24-160-GGSGSG-192-221) , Rpn8(1-179)

Depositor

Andreas Martin

Article

Worden et al Mol Cell. 2017 Sep 7;67(5):799-811.e8. d

Inserts

Rpn11 (RPN11 Budding Yeast)
rpn8 (RPN8 Budding Yeast)

Tags

His-Prescission

Expression

Bacterial

Mutation

1-179 and amino acids 24-160-GGSGSG-192-221

Promoter

T7

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-C1-AKT-PH

Plasmid

#21218

Depositor

Tobias Meyer

Article

Raucher et al Cell. 2000 Jan 21. 100(2):221-8.

Insert

AKT-PH

Tags

GFP

Expression

Mammalian

Mutation

PH domain of AKT. (Lab plasmid WC 0002).

Available Since

July 10, 2009

Availability

Academic Institutions and Nonprofits only

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pHES609 [prNOp7-PBAC]

Plasmid

#103017

Purpose

A S. cerevisiae plasmid. A photosensitive adenylate cyclase that produces cAMP in response to blue light. Used to activate PKA.

Depositor

Hana El-Samad

Article

Stewart-Ornstein et al Mol Biol Cell. 2017 Jan 1;28(1):221-227.

Insert

bPAC

Expression

Yeast

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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pME tdTomato-NLS

Plasmid

#135205

Purpose

Red fluorescent tag

Depositor

David Tobin

Insert

tdTomato

Use

Synthetic Biology

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pM2AH + mSlo1

Plasmid

#16152

Depositor

Larry Salkoff

Article

Butler et al Science. 1993 Jul 9. 261(5118):221-4.

Insert

Mus musculus potassium large conductance calcium-activated channel (Kcnma1 Mouse)

Expression

Mammalian

Mutation

N/A

Available Since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only
pETSUMO2_Bacteroidetes-bGSDM

Plasmid

#212124

Purpose

For expression of the bacterial gasdermin (bGSDM) encoded by a Bacteroidetes (metagenomic) species. Protein is expressed with an N-terminal SUMO2 tag.

Depositor

Philip Kranzusch

Article

Johnson et al Science. 2022 Jan 14;375(6577):221-225.

Insert

Bacteroidetes bGSDM

Tags

6xHis-SUMO2

Expression

Bacterial

Mutation

N-terminal methionine deleted and replaced by sin…

Promoter

T7

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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