We narrowed to 9,663 results for: BLI

1. pET28-3'TwStrep

   Plasmid

   #208053

   Purpose

   Empty vector for prokaryotic expression, insert a Twin Strep tag (with TAA) between HindII and XhoI site of pET28a (+), both HindIII and XhoI are retained. HindIII in-frame with Twin Strep tag.

   Depositor

   Quanfu Ma

   Type

   Empty backbone

   Tags

   Twin Strep

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET28-5'TwStrep

   Plasmid

   #208620

   Purpose

   Empty vector for prokaryotic expression, insert a Twin Strep tag between NdeI and BamHI site of pET28a (+), both site retained, T7 tag replaced, BamHI site in-frame with Twin Strep tag.

   Depositor

   Quanfu Ma

   Type

   Empty backbone

   Tags

   Twin Strep

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. SNX32A-c014

   Plasmid

   #175486

   Purpose

   Protein expression in bacterial cells. PX domain, S17-M197

   Depositor

   Opher Gileadi

   Insert

   SNX32 (SNX32 Human)
   

   Tags

   His6-SUMO

   Expression

   Bacterial

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. FU_tetO_dCas9-VP192-T2A-EGFP

   Plasmid

   #121173

   Purpose

   Lentiviral construct for inducible expression of dCas9-VP192 transcriptional activator and EGFP.

   Depositor

   Timo Otonkoski

   Insert

   dCas9-VP192-T2A-EGFP
   

   Use

   CRISPR and Lentiviral; Inducible by doxycycline

   Tags

   T2A-EGFP-ires-puro

   Expression

   Mammalian

   Available Since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.1-hUCP4-NE

   Plasmid

   #102362

   Purpose

   Overexpression of solute carrier family 25 member 27 in mammalian cells

   Depositor

   Shu Leong Ho

   Insert

   solute carrier family 25 member 27 (SLC25A27 Human)
   

   Tags

   NE

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Optopatch2 (QuasAr2-mOrange2-P2A-CheRiff-eGFP)

   Plasmid

   #51656

   Purpose

   hsyn promoting optopatch, which includes a ChR64, GFP, Quasar2 and mOrange

   Depositor

   Adam Cohen

   Insert

   Quasar2-mOrange-Chr64-GFP
   

   Use

   Lentiviral

   Tags

   GFP and mOrange

   Expression

   Mammalian

   Promoter

   human synapsin

   Available Since

   March 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBS L30 mRuby3-Rab10

   Plasmid

   #166429

   Purpose

   Rab10 fused to mRuby3 fluorescent protein (ex558/em592) tag in N-ter under control of a weak L30 promoter.

   Depositor

   Raphael Gaudin

   Insert

   Rab10
   

   Tags

   mRuby3

   Expression

   Mammalian

   Promoter

   L30 weak promoter

   Available Since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pKG141

   Plasmid

   #69759

   Purpose

   Retroviral expression of CENP-B

   Depositor

   Iain Cheeseman

   Insert

   CENP-B (CENPB Human)
   

   Use

   Retroviral

   Tags

   eGFP-TEV-S

   Expression

   Mammalian

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

9. DFNB31-TwinStrepII

   Plasmid

   #83196

   Purpose

   Expression of tagged protein in mammalian cells

   Depositor

   Peter Barr-Gillespie

   Insert

   DFNB31 (Whrn Mouse)
   

   Tags

   Twin Strep Tag II

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-Ef1a-vCreDIO hChR2(H134R)-eYFP 2.0

    Plasmid

    #126081

    Purpose

    vCre-Dependent ChR2(H134R)-eYFP with the vLox sites in the reverse orientation

    Depositor

    Karl Deisseroth

    Insert

    hChR2(H134R)-eYFP
    

    Use

    AAV

    Tags

    eYFP

    Mutation

    H134R

    Promoter

    Ef1a

    Available Since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pmSod1-WT-N’His

    Plasmid

    #117701

    Purpose

    Expression of WT mouse SOD1 with N- terminal His tag

    Depositor

    Elizabeth Fisher

    Insert

    Sod1 (Sod1 Mouse)
    

    Tags

    His tag with Thrombin cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSB700-mouse-TTN-Cerulean

    Plasmid

    #128325

    Purpose

    SP-dCas9 compatible gRNA to be used as a positive control for activation experiments (mouse cells)

    Depositor

    Alejandro Chavez

    Insert

    gRNA against mouse TTN for activation
    

    Expression

    Mammalian

    Available Since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1_C-6His-Stx17

    Plasmid

    #112259

    Purpose

    C-term His tagged pcDNA3.1 vector expressing mouse Stx17 (syntaxin 17)

    Depositor

    Madhulika Tripathi

    Insert

    syntaxin 17 (Stx17 Mouse)
    

    Tags

    6His Tag

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1-hSyncytin-1(del480)

    Plasmid

    #182441

    Purpose

    hSyncytin-1, deletion 481-538

    Depositor

    Masaharu Somiya

    Insert

    ERVW-1 (ERVW-1 Human)
    

    Expression

    Mammalian

    Mutation

    deletion 481-538

    Promoter

    CMV

    Available Since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PX380 Treponema denticola Cas9

    Plasmid

    #68317

    Purpose

    Treponema denticola Cas9

    Depositor

    Feng Zhang

    Insert

    TdCas9
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pShuttle-CMV-F2A-T2A-Venus

    Plasmid

    #62621

    Purpose

    AdEasy Shuttle vector modified for multicistronic expression. Contains T2A, F2A and Venus tag

    Depositor

    Zheng-Xu Wang

    Insert

    MCS with F2A and T2A
    

    Use

    Adenoviral

    Tags

    Venus

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. TTC5

    Plasmid

    #39064

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    TTC5 (TTC5 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pJK407

    Plasmid

    #72421

    Purpose

    Produces Acetobacter aceti 1023 Est06, C-terminal His6 tag

    Depositor

    T. J. Kappock

    Insert

    lipase
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    lacks Met1 and Thr2 in annotated (v1) sequence

    Promoter

    T7

    Available Since

    Feb. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p-dCas9-VP64-Hygro

    Plasmid

    #104405

    Purpose

    transient expression of dCas9-VP64 fusion protein

    Depositor

    Stephan Beck

    Insert

    VP64
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 371-GFP

    Plasmid

    #97030

    Purpose

    His6-GFP-tev-yORF

    Depositor

    Chris Jeans

    Type

    Empty backbone

    Tags

    GFP fusion, His6 tag, and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge