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pMSR:MSMEG_0301:Dendra

Plasmid

#159050

Purpose

T7 expression of C-term fluorescent fusion in E. coli

Depositor

Keith Derbyshire, Joseph Wade

Article

Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

Insert

MSMEG_0301

Use

Integrating mycobacterial expression vector attl5

Tags

Gly-Ala Linker/Dendra2/1x Flag Tag

Expression

Bacterial

Promoter

pmycTetO

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pMSR:MSMEG_0225:Dendra

Plasmid

#159043

Purpose

T7 expression of C-term fluorescent fusion in E. coli

Depositor

Keith Derbyshire, Joseph Wade

Article

Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

Insert

MSMEG_0225

Use

Integrating mycobacterial expression vector attl5

Tags

Gly-Ala Linker/Dendra2/1x Flag Tag

Expression

Bacterial

Promoter

pmycTetO

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pMSR:MSMEG_0710:Dendra

Plasmid

#159020

Purpose

T7 expression of C-term fluorescent fusion in E. coli

Depositor

Keith Derbyshire, Joseph Wade

Article

Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

Insert

MSMEG_0710

Use

Integrating mycobacterial expression vector attl5

Tags

Gly-Ala Linker/Dendra2/1x Flag Tag

Expression

Bacterial

Promoter

pmycTetO

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pMSR:MSMEG_0384:Dendra

Plasmid

#159013

Purpose

T7 expression of C-term fluorescent fusion in E. coli

Depositor

Keith Derbyshire, Joseph Wade

Article

Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

Insert

MSMEG_0384

Use

Integrating mycobacterial expression vector attl5

Tags

Gly-Ala Linker/Dendra2/1x Flag Tag

Expression

Bacterial

Promoter

pmycTetO

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pMSR:MSMEG_0229:Dendra

Plasmid

#159005

Purpose

T7 expression of C-term fluorescent fusion in E. coli

Depositor

Keith Derbyshire, Joseph Wade

Article

Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

Insert

MSMEG_0229

Use

Integrating mycobacterial expression vector attl5

Tags

Gly-Ala Linker/Dendra2/1x Flag Tag

Expression

Bacterial

Promoter

pmycTetO

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pMSR:MSMEG_0024:Dendra

Plasmid

#158997

Purpose

T7 expression of C-term fluorescent fusion in E. coli

Depositor

Keith Derbyshire, Joseph Wade

Article

Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

Insert

MSMEG_0024

Use

Integrating mycobacterial expression vector attl5

Tags

Gly-Ala Linker/Dendra2/1x Flag Tag

Expression

Bacterial

Promoter

pmycTetO

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pEN_hU6miR-Gb2-K

Plasmid

#25759

Purpose

Entry vector with human U6 promoter driving both mouse G alpha 12 and G alpha 13 miR30-based shRNAs.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

Gb2 miR-shRNA (Gnb2 Mouse)

Use

Gateway Cloning

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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CRISPRi non-coding library (CRiNCL) - Common to all 7 cell lines

Pooled Library

#86538

Depositor

Jonathan Weissman

Article

Liu et al Science. 2017 Jan 06. Vol. 355, Issue 63

Available Since

March 8, 2017

Availability

Academic Institutions and Nonprofits only
Lenti-human-UBDUB-gRNA-puromycin-IRES-CFP

Pooled Library

#171531

Purpose

Knockout library targeting ubiquitination and de-ubiquitination complex genes.

Depositor

Chunliang Li

Article

Wright et al Proc Natl Acad Sci U S A. 2023 Apr 18;12

Expression

Mammalian

Species

Homo sapiens

Use

Lentiviral

Available Since

June 22, 2021

Availability

Academic Institutions and Nonprofits only
Mouse Metabolic Gene CRISPRa sgRNA library

Pooled Library

#187081

Purpose

Library targeting 2,989 mouse metabolic genes for activation of gene expression.

Depositor

Kivanc Birsoy

Article

Unlu et al Mol Cell. 2022 Aug 4;82(15):2832-2843.e7

Expression

Mammalian

Species

Mus musculus

Use

Lentiviral

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only
mTF-CRISPRai

Pooled Library

#122131

Purpose

Increases transcriptional repression of genes encoding transcription factors in combination with dCas9 repression systems, such as the dCas9-KRAB system.

Depositor

Stanley Qi

Article

Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

Expression

Mammalian

Species

Mus musculus

Use

CRISPR and Lentiviral

Available Since

April 25, 2019

Availability

Academic Institutions and Nonprofits only
RLC12

Pooled Library

#163954

Purpose

Targets open reading frames and non-coding RNAs in M. tuberculosis.

Depositor

Jeremy Rock

Article

Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

Expression

Bacterial

Use

CRISPR

Available Since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only
pAAV-TRE-DIO-eYFP

Plasmid

#117383

Purpose

An AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein eYFP

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Has Service

AAV1

Insert

EYFP

Use

AAV

Expression

Mammalian

Promoter

TRE

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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Human Lipid Droplet and Metabolism Library

Pooled Library

#191535

Purpose

Targets 1,196 genes related to lipid droplet biology and cell metabolism.

Depositor

James Olzmann

Article

Roberts et al Dev Cell. 2023 Jul 18:S1534-5807(23)0033

Expression

Mammalian

Use

CRISPR and Lentiviral

Available Since

March 1, 2023

Availability

Academic Institutions and Nonprofits only
Green monkey (Chlorocebus sabaeus) sgRNA library

Pooled Library

#178284

Purpose

Knockout library targeting the green monkey (Chlorocebus sabaeus) genome.

Depositor

John Doench, David Root

Article

Wei et al Cell. 2021 Jan 7;184(1):76-91.e13. doi:

Expression

Mammalian

Species

Chlorocebus sabaeus (african green monkey)

Use

CRISPR and Lentiviral

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only
Mouse messenger-RBP sgRNA library

Pooled Library

#169082

Purpose

Retroviral knockout library targeting mouse mRNA binding proteins with 10 sgRNAs/gene.

Depositor

Martin Turner

Article

Turner et al eLife. 2022 Apr 22;11. pii: 72313. doi:

Expression

Mammalian

Species

Mus musculus

Use

CRISPR and Lentiviral

Available Since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only
Lourido Toxoplasma CRISPR Library V1

Pooled Library

#80636

Purpose

Knockout library targeting 8,158 predicted protein-coding gene in Toxoplasma gondii.

Depositor

Sebastian Lourido

Article

Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

Available Since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only
RLC11

Pooled Library

#163955

Purpose

Targets open reading frames and non-coding RNAs in M. smegmatis.

Depositor

Jeremy Rock

Article

Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

Expression

Bacterial

Use

CRISPR

Available Since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only
pET-LiCre

Plasmid

#219797

Purpose

Expresses a codon-optimized His-tagged Light-inducible Cre (LiCre) recombinase in E. coli. Plasmid ID in Yvert lab is pGY611. LiCre is described in https://doi.org/10.7554/eLife.61268

Depositor

Gaël Yvert

Article

Dufour et al Biol Open. 2026 May 15;15(5):bio062381.

Insert

Codon-optimized Light-inducible Cre Recombinase

Use

Cre/Lox

Tags

HIS-tag for purification

Expression

Bacterial

Promoter

IPTG-inducible

Available Since

May 20, 2026

Availability

Academic Institutions and Nonprofits only

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Ingolia lab S cerevisiae CRISPRi v1 – barcodes 181005

Pooled Library

#161769

Purpose

Tet-inducible inhibition library targeting the yeast genome with barcode linked guide RNAs.

Depositor

Nicholas Ingolia

Article

McGlincy et al BMC Genomics. 2021 Mar 23;22(1):205. doi

Expression

Yeast

Species

Saccharomyces cerevisiae

Use

CRISPR

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only