164,824 results

1. CROPseq-EFS-HypaSpCas9-P2A-EGFP

   Plasmid

   #106964

   Purpose

   single-vector CROPseq system with high fidelity SpCas9 (HypaSpCas9) for use in the CROPseq CRISPR knockout screen

   Depositor

   Alex Hewitt

   Insert

   HypaSpCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. Anti-GFP [N86/38.1R]

   Plasmid

   #114492

   Purpose

   Mammalian Expression Plasmid of anti-GFP. Derived from hybridoma N86/38.1.

   Depositor

   James Trimmer

   Article

   Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

   Insert

   anti-GFP (Aequorea victoria) recombinant mouse monoclonal antibody
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   dual CMV

   Available since

   May 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLVX-EF1alpha-2xGFP:NES-IRES-2xRFP:NLS

   Plasmid

   #71396

   Purpose

   2Gi2R assay

   Depositor

   Fred Gage

   Article

   Mertens et al Cell Stem Cell. 2015 Oct 6. pii: S1934-5

   Inserts

   2xGFP:NES
   2xRFP:NLS
   

   Use

   Lentiviral

   Tags

   IRES

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha and IRES

   Available since

   Dec. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. Emerin pEGFP-N2 (588)

   Plasmid

   #61985

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

   Insert

   Emerin (EMD Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUltra-U6-gRNAs1-5

   Plasmid

   #178569

   Purpose

   Constitutive expression of a gRNA-tRNA array for macsGESTALT editing

   Depositor

   Christopher Lengner

   Article

   Simeonov et al Cancer Cell. 2021 Aug 9;39(8):1150-1162.

   Insert

   5 gRNA-tRNA array with U6 promoter
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-CAG-GFP (AAV PHP.eB)

   Viral Prep

   #37825-PHPeB

   Purpose

   Ready-to-use AAV PHP.eB particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA. CAG-driven GFP expression. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Edward Boyden

   Promoter

   CAG

   Tags

   GFP

   Available since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

7. Cbh_v5 AAV-CBE N-terminal

   Plasmid

   #137175

   Purpose

   AAV genome: expresses the N-terminal of v5 AAV-CBE from the Cbh promoter, and U6-sgRNA

   Depositor

   David Liu

   Article

   Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

   Insert

   v5 AAV-CBE N-terminal; U6-protospacer
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Cas9 D10A

   Promoter

   Cbh

   Available since

   Jan. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. NbV5-eGFP

   Plasmid

   #201473

   Purpose

   Indirect microscopy/imaging of V5-tagged protein

   Depositor

   Patrick Giguère

   Article

   Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

   Insert

   NbV5-eGFP
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV_BiPVe3_dTomato_nlsdTomato (AAV PHP.eB)

   Viral Prep

   #213940-PHPeB

   Purpose

   Ready-to-use AAV PHP.eB particles produced from pAAV_BiPVe3_dTomato_nlsdTomato (#213940). In addition to the viral particles, you will also receive purified pAAV_BiPVe3_dTomato_nlsdTomato plasmid DNA. Bicistronic expression of dTomato and nuclear-targeted dTomato under the control of the PV+ basket cell-targeting enhancer E3. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Gordon Fishell

   Article

   Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

   Promoter

   Tags

   dTomato

   Available since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

10. COM-epegRNA-Dnmt1

    Plasmid

    #211376

    Purpose

    Dnmt1 targeting epegRNA with COM insertion in gRNA scaffold for v3b PE-eVLP production

    Depositor

    David Liu

    Article

    An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

    Insert

    COM-epegRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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