169,450 results
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Plasmid#164819PurposeReporter to measure ISR state-dependent protein synthesisDepositorInsertSPOTlight-U2GCR
UseAAVExpressionMammalianPromoterCAGAvailable SinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV-pCbh-SpRY[C-term]-gRNAentry (CA20)
Plasmid#197511PurposeCbh promoter expression plasmid for C-terminal intein-split AAV construct with C-term of SpRY and BsmBI entry cassette to clone SpCas9 gRNA spacerDepositorInsertAAV-[ITR]-pCbh-BPNLS-NpuC-SpRY[C-term]-BPNLS-gRNA[BsmBI]-pU6-[ITR]
UseAAV and CRISPRTagsBPNLS and BPNLS-NpuC(intein)MutationC-terminal mutations of SpRY(L1111R/D1135L/S1136W…PromoterCbhAvailable SinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
MoClo Yeast Secretion and Display (YSD) Toolkit
Plasmid Kit#1000000240PurposeThe YSD Toolkit is a collection of 34 plasmids that can be used in conjunction with the MoClo Yeast Toolkit (YTK) to optimize secretion and cell surface display of proteins of interest in yeast.DepositorAvailable SinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-Jaws-KGC-GFP-ER2 (AAV8)
Viral Prep#65014-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-hsyn-Jaws-KGC-GFP-ER2 (#65014). In addition to the viral particles, you will also receive purified pAAV-hsyn-Jaws-KGC-GFP-ER2 plasmid DNA. hSyn-driven Jaws-GFP expression for optogenetic neural inhibition. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsGFPAvailable SinceOct. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
p11-LacY-wtx1
Plasmid#69056PurposeThe reporter plasmid p11-LacY-wtx1 encodes the toxin CcdB gene under the inducible BAD promoter and a single copy of the endonuclease cleavage site which is readily extendable to multiple copies.DepositorAvailable SinceSept. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCRIS-PITChv2-Puro-dTAG (BRD4)
Plasmid#91793PurposePITCh dTAG donor vector for Puro-P2A-2xHA-FKBP_F36V knock-in into the N-terminus of the human BRD4 locus.DepositorInsertPuro-P2A-2xHA-FKBP_F36V
UseCRISPRExpressionMammalianMutationPhenylalanine 36 to valine in FKBP12PromoterPromotorlessAvailable SinceMarch 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCas9
Plasmid#42876PurposeBacterial expression of Cas9 nuclease, tracrRNA and crRNA guideDepositorInserttracr/Cas9
UseCRISPR; E.coliExpressionBacterialAvailable SinceFeb. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Golgi-7
Plasmid#55052PurposeLocalization: Golgi Complex, Excitation: 587, Emission: 610DepositorInsertGolgi (B4GALT1 Human)
TagsmCherryExpressionMammalianMutationaa 1-82 of NM_001497.3PromoterCMVAvailable SinceOct. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
ERK-KTR-mStrawberry reporter
Plasmid#158686PurposeThis plasmid is a ERK pathway reporter. It has CMV promoter driving the expression of ERK-KTR fused to mStrawberry-pGK-BSDDepositorInsertmStrawberry
UseLentiviralExpressionMammalianPromoterCMVAvailable SinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
mCherry-pBAD
Plasmid#54630PurposeLocalization: Protein Expression Vector , Excitation: 587, Emission: 610DepositorTypeEmpty backboneTags6xHis and mCherryExpressionBacterialAvailable SinceJuly 25, 2014AvailabilityAcademic Institutions and Nonprofits only