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pWUKI Cas1 P279L Cas2

Plasmid

#80095

Purpose

Encodes Cas1 P279L and Cas2

Depositor

George Church

Article

Shipman et al Science. 2016 Jun 9. pii: aaf1175.

Inserts

Cas1 (cas1 )
Cas2 (cas2 )
CRISPR Array

Expression

Bacterial

Mutation

P279L

Available Since

Jan. 25, 2018

Availability

Academic Institutions and Nonprofits only

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Bxbi+TP901 GFP Bandpass BAC Reporter

Plasmid

#78229

Purpose

synthetic gene circuit

Depositor

Timothy Lu

Article

Rubens et al Nat Commun. 2016 Jun 3;7:11658. doi: 10.

Insert

synthetic circuit

Available Since

May 11, 2017

Availability

Academic Institutions and Nonprofits only

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pET-21a(+)-AroQ

Plasmid

#12682

Depositor

SeWon Suh

Article

Lee et al Proteins. 2003 Jun 1. 51(4):616-7.

Insert

type II dehydroquinase (HP1038 Helicobacter pylori)

Tags

His

Expression

Bacterial

Available Since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only

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p230 pCMVe-betaAc-luc

Plasmid

#8391

Depositor

Jeffrey Green

Article

Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

Insert

CMVe-betaAc

Use

Luciferase

Expression

Mammalian

Available Since

Dec. 29, 2005

Availability

Academic Institutions and Nonprofits only
M50-IRES-GFP-IRES-tauLacZ LNL TV

Plasmid

#15646

Depositor

Peter Mombaerts

Article

Feinstein et al Cell. 2004 Jun 11. 117(6):817-31.

Insert

M50-IRES-GFP-IRES-tauLacZ LNL TV

Use

High copy number

Available Since

Aug. 24, 2007

Availability

Academic Institutions and Nonprofits only
p138

Plasmid

#8384

Depositor

Jeffrey Green

Article

Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

Insert

LOX I

Use

Cre/Lox

Tags

EGFP

Expression

Mammalian

Available Since

April 28, 2006

Availability

Academic Institutions and Nonprofits only
OE2-M71

Plasmid

#15630

Depositor

Peter Mombaerts

Article

Feinstein et al Cell. 2004 Jun 11. 117(6):833-46.

Insert

OE2-M71-IRES-tauGFP ACNF TV

Use

High copy number

Available Since

Aug. 24, 2007

Availability

Academic Institutions and Nonprofits only
pAVEC0_Empty

Plasmid

#243799

Purpose

pAVEC0 backbone with no fluorophores or tags

Depositor

Jacob Brunkard

Article

Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

Type

Empty backbone

Use

Agrobacterium mediated transformation

Expression

Plant

Promoter

35S

Available Since

Dec. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pDASH-DI-α2

Plasmid

#242516

Purpose

Donor I vector in DASH and alpha2 vector in GoldenBraid/DASH, with attBTT and attBCC-FRT

Depositor

Jose Alonso, Anna Stepanova

Article

Zhao et al Plant Biotechnol J. 2025 Jun 10. doi: 10

Type

Empty backbone

Use

Synthetic Biology

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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ISA861: Melezitose pathway

Plasmid

#241971

Purpose

Melezitose pathway phagemid. Contains p15A, CmR, pac site, AraC, C1, and pBAD.

Depositor

Nathan Crook

Article

Al'Abri et al Nucleic Acids Res. 2022 Jun 10;50(10):e5

Insert

melezitose pathway

Use

Synthetic Biology; Phagemid

Expression

Bacterial

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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ISA894: vioABCDE phagemid

Plasmid

#241972

Purpose

coi(Y35H) phagemid with higher infection rate (unpublished)

Depositor

Nathan Crook

Article

Al'Abri et al Nucleic Acids Res. 2022 Jun 10;50(10):e5

Insert

vioABCDE

Use

Synthetic Biology; Phagemid

Expression

Bacterial

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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ISA410: sfGFP

Plasmid

#241969

Purpose

Phagemid for SC101 and sfGFP. Contains p15A, CmR, pac site, AraC, C1, and pBAD.

Depositor

Nathan Crook

Article

Al'Abri et al Nucleic Acids Res. 2022 Jun 10;50(10):e5

Insert

pSC101-sfGFP

Use

Synthetic Biology; Phagemid

Expression

Bacterial

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pEP28

Plasmid

#229235

Purpose

Empty backbone tet operator and TetR

Depositor

Yolanda Schaerli

Article

Pignon et al ISME J. 2025 Jun 13:wraf122. doi: 10.109

Type

Empty backbone

Expression

Bacterial

Available Since

June 11, 2025

Availability

Academic Institutions and Nonprofits only

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POC1423

Plasmid

#221559

Purpose

Plasmid designed to assay the site-selective methylation protection approach using the non-switchable methylases

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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POC1343

Plasmid

#221568

Purpose

Plasmid containing the Insert 1 to be assembled in POC1355

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DNA fragment flanked by BsaI sites

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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POC1344

Plasmid

#221569

Purpose

Plasmid containing the Insert 2 to be assembled in POC1355

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DNA fragment flanked by BsaI sites

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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POC1345

Plasmid

#221570

Purpose

Plasmid containing the Insert 3 to be assembled in POC1355

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DNA fragment flanked by BsaI sites

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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POC1346

Plasmid

#221571

Purpose

Plasmid containing the Insert 4 to be assembled in POC1355

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DNA fragment flanked by BsaI sites

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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pLEU

Plasmid

#213846

Purpose

LEU integrating plasmid backbone used to create all of the reporter plasmids.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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KNtp-SUR1 core fusion

Plasmid

#216212

Purpose

for expression of KNtp-SUR1 fusion protein in BacMam system

Depositor

Lei Chen

Article

Wang et al Front Pharmacol. 2022 Jun 30;13:929684.

Insert

SUR1 core

Tags

GFP-MBP

Expression

Mammalian

Mutation

deletion of TMD0

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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