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pJexpress414_OmpA171_R4cons_G41A

Plasmid

#64299

Purpose

Conservative version of OmpA Redesign 4 with G41A mutation

Depositor

Timothy Whitehead

Article

Stapleton et al Proc Natl Acad Sci U S A. 2015 Jul 21. p

Insert

OmpA_R4cons_G41A

Use

Synthetic Biology

Tags

6His and FlAsH

Expression

Bacterial

Promoter

T7

Available Since

May 27, 2015

Availability

Academic Institutions and Nonprofits only
pJexpress414_OmpA171_R4cons

Plasmid

#62639

Purpose

Conservative version of OmpA Redesign 4

Depositor

Timothy Whitehead

Article

Stapleton et al Proc Natl Acad Sci U S A. 2015 Jul 21. p

Insert

OmpA_R4cons

Use

Synthetic Biology

Tags

6His and FlAsH

Expression

Bacterial

Mutation

25 mutations to lipid-facing surface

Promoter

T7

Available Since

May 27, 2015

Availability

Academic Institutions and Nonprofits only
piHGpd/P82

Plasmid

#1325

Depositor

Susan Lindquist

Article

Nathan et al Proc Natl Acad Sci U S A 1997 Nov 25;94(

Insert

GPD promoter-HSP82 (HSP82 Budding Yeast)

Expression

Yeast

Mutation

Wild type gene

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
K218Tc-3

Plasmid

#1300

Depositor

Susan Lindquist

Article

Schirmer et al Proc Natl Acad Sci U S A 2001 Jan 30;98(

Insert

mutant HSP104 (HSP104 Budding Yeast)

Expression

Yeast

Mutation

K218T: A315T

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
pSL012

Plasmid

#246580

Purpose

express mGreen Lantern from the cadBA promoter

Depositor

Tina Wang

Article

Li et al Proc Natl Acad Sci U S A. 2025 Sep 16;12

Insert

mGreenLantern

Expression

Bacterial

Available Since

Dec. 4, 2025

Availability

Academic Institutions and Nonprofits only

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PTGR-PS2

Plasmid

#237442

Purpose

Expressing PS2 S-layer from C. glutamicum under its native promoter

Depositor

Han Remaut

Article

Sogues et al Proc. Natl. Acad. Sci. U.S.A. 122 (31) e

Insert

cpsB

Expression

Bacterial

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pCTcon2-dHUH

Plasmid

#235187

Purpose

To display dHUH on yeast surface

Depositor

Alice Ting

Article

Huang et al Proc Natl Acad Sci U S A. 2025 Mar 4;122

Insert

dHUH

Tags

HA, aga2p, and myc

Expression

Yeast

Available Since

March 31, 2025

Availability

Academic Institutions and Nonprofits only

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pAM259

Plasmid

#226903

Purpose

mEOS3.2-MBP-Pex15(1-253)-PSP-FLAG-6xHis

Depositor

Andreas Martin

Article

Castanzo et al Proc Natl Acad Sci U S A. 2020 Jun 30;11

Insert

mEOS3.2

Tags

mEOS3.2-MBP-Pex15(1-253)-PSP-FLAG-6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAM244

Plasmid

#226888

Purpose

6xHis-PSP-GS-Msp1(36-362)

Depositor

Andreas Martin

Article

Castanzo et al Proc Natl Acad Sci U S A. 2020 Jun 30;11

Insert

msp1 (MSP1 Budding Yeast)

Tags

His-Precission

Expression

Bacterial

Promoter

T7

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pTrc-CysGA-Im7 WT

Plasmid

#201081

Purpose

Im7 WT inserted into CysGA at G364 via GS linker

Depositor

Shu Quan

Article

Ren et al Proc Natl Acad Sci U S A. 2021 Mar 30;11

Insert

CysGA-Im7

Expression

Bacterial

Promoter

pTrc

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pTrc-CysGA-Linker only

Plasmid

#201080

Purpose

GS linker inserted into CysGA at G364

Depositor

Shu Quan

Article

Ren et al Proc Natl Acad Sci U S A. 2021 Mar 30;11

Insert

CysGA-Linker only

Expression

Bacterial

Promoter

pTrc

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pRegev_D3

Plasmid

#204182

Purpose

Single reporter with long guanine depleted (G-poor) region from splicing library.

Depositor

Oded Regev

Article

Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

Insert

Exon D3

Use

Synthetic Biology

Expression

Mammalian

Promoter

Truncated CAG

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pRegev_S5

Plasmid

#204174

Purpose

Single reporter with secondary structure from splicing library.

Depositor

Oded Regev

Article

Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

Insert

Exon S5 in beta globin splicing minigene

Use

Synthetic Biology

Expression

Mammalian

Promoter

Truncated CAG

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pRegev_S1

Plasmid

#204150

Purpose

Single reporter with secondary structure from splicing library.

Depositor

Oded Regev

Article

Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

Insert

S1 exon in beta globin splicing minigene

Use

Synthetic Biology

Expression

Mammalian

Promoter

Truncated CAG

Available Since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pRegev_S4

Plasmid

#204170

Purpose

Single reporter with secondary structure from splicing library.

Depositor

Oded Regev

Article

Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

Insert

Exon S4 in beta globin splicing minigene

Use

Synthetic Biology

Expression

Mammalian

Promoter

Truncated CAG

Available Since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pRegev_S2

Plasmid

#204162

Purpose

Single reporter with secondary structure from splicing library.

Depositor

Oded Regev

Article

Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

Insert

S2 exon in beta globin splicing minigene

Use

Synthetic Biology

Expression

Mammalian

Promoter

Truncated CAG

Available Since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pUC19_BAS1509

Plasmid

#82354

Purpose

T7 in vitro transcription template for Bas mRNA

Depositor

Nils Walter

Article

Chatterjee et al Proc Natl Acad Sci U S A. 2021 Apr 20;11

Insert

BAS1509 hypothetical protein

Use

Unspecified

Promoter

T7

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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plIVMD217

Plasmid

#160939

Purpose

Excess Capsid Protein in vivo mRNA display

Depositor

Saeed Tavazoie

Article

Oikonomou et al Proc Natl Acad Sci U S A. 2020 Oct 9. pi

Insert

MCP-mTagBFP2-HA Tag

Tags

HA tag

Expression

Yeast

Promoter

MET25

Available Since

Nov. 13, 2020

Availability

Academic Institutions and Nonprofits only

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plIVMD118

Plasmid

#160938

Purpose

Excess Capsid Protein in vivo mRNA display

Depositor

Saeed Tavazoie

Article

Oikonomou et al Proc Natl Acad Sci U S A. 2020 Oct 9. pi

Insert

MCP-mCherry-FLAG tag

Tags

MYC tag

Expression

Yeast

Promoter

MET25

Available Since

Nov. 13, 2020

Availability

Academic Institutions and Nonprofits only

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plIVMD115

Plasmid

#160937

Purpose

Excess Capsid Protein for in vivo mRNA display

Depositor

Saeed Tavazoie

Article

Oikonomou et al Proc Natl Acad Sci U S A. 2020 Oct 9. pi

Insert

MCP-mCherry-MYC tag

Tags

MYC tag

Expression

Yeast

Promoter

MET25

Available Since

Nov. 13, 2020

Availability

Academic Institutions and Nonprofits only

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GtCCR1_EYFP_pcDNA3.1

Plasmid

#159120

Purpose

This plasmid encodes GtCCR1 rhodopsin domain

Depositor

John Spudich

Article

Sineshchekov et al Proc Natl Acad Sci U S A. 2017 Nov 7;114

Insert

GtCCR1

Tags

EYFP

Expression

Mammalian

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pKEW395_AcrIIA14_truncated

Plasmid

#138284

Purpose

Expression of truncated AcrIIA14 protein

Depositor

Jennifer Doudna

Article

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

truncated AcrIIA14

Use

CRISPR

Tags

MBP

Expression

Bacterial

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pKEW353_AcrIIA13_truncated

Plasmid

#138282

Purpose

Expression of truncated AcrIIA13 protein

Depositor

Jennifer Doudna

Article

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

truncated AcrIIA13

Use

CRISPR

Tags

MBP

Expression

Bacterial

Available Since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

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pKEW398_AcrIIA15_truncated

Plasmid

#138286

Purpose

Expression of truncated AcrIIA15 protein

Depositor

Jennifer Doudna

Article

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

AcrIIA15

Use

CRISPR

Tags

SFGFP

Expression

Bacterial

Available Since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

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pJexpress414_OmpA171_R4cons_Y129H

Plasmid

#64302

Purpose

Conservative version of OmpA Redesign 4 with Y129H mutation

Depositor

Timothy Whitehead

Article

Stapleton et al Proc Natl Acad Sci U S A. 2015 Jul 21. p

Insert

OmpA_R4cons_Y129H

Use

Synthetic Biology

Tags

6His and FlAsH

Expression

Bacterial

Mutation

25 mutations to lipid-facing surface

Promoter

T7

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only
pJexpress414_OmpA171_H3478

Plasmid

#64292

Purpose

OmpA redesign hybrid with wild-type strands 3478

Depositor

Timothy Whitehead

Article

Stapleton et al Proc Natl Acad Sci U S A. 2015 Jul 21. p

Insert

OmpA_H3478

Use

Synthetic Biology

Tags

6His and FlAsH

Expression

Bacterial

Promoter

T7

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only
pJexpress414_OmpA171_H346

Plasmid

#64312

Purpose

OmpA redesign hybrid with wild-type strands 346

Depositor

Timothy Whitehead

Article

Stapleton et al Proc Natl Acad Sci U S A. 2015 Jul 21. p

Insert

OmpA_H346

Use

Synthetic Biology

Tags

6His and FlAsH

Expression

Bacterial

Promoter

T7

Available Since

July 16, 2015

Availability

Academic Institutions and Nonprofits only
pCVD630

Plasmid

#66163

Purpose

cloning of ace gene

Depositor

James Kaper

Article

Trucksis et al Proc Natl Acad Sci U S A. 1993 Jun 1;90(

Insert

ace

Use

Cloning vector

Promoter

Unknown

Available Since

June 18, 2015

Availability

Academic Institutions and Nonprofits only
pCVD453

Plasmid

#66154

Purpose

cloning of E. coli E2348/69 left end of the LEE region, contains rorf1 and espG.

Depositor

James Kaper

Article

McDaniel et al Proc Natl Acad Sci U S A. 1995 Feb 28;92

Insert

LEE probe A rorf1, espG

Use

Cloning vector

Promoter

none

Available Since

June 18, 2015

Availability

Academic Institutions and Nonprofits only
pJexpress414_OmpA171_H-78

Plasmid

#64313

Purpose

OmpA redesign hybrid with wild-type strands 123456

Depositor

Timothy Whitehead

Article

Stapleton et al Proc Natl Acad Sci U S A. 2015 Jul 21. p

Insert

OmpA_H-78

Use

Synthetic Biology

Tags

6His and FlAsH

Expression

Bacterial

Promoter

T7

Available Since

May 27, 2015

Availability

Academic Institutions and Nonprofits only