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Plasmid#109237PurposePTac driving GFPDepositorInsertsfGFP
UseTagsExpressionBacterialMutationPromoterPTacAvailable sinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
A3Bi-ctd E255A -Cas9n-UGI-NLS
Plasmid#109429PurposeExpresses the C-terminal catalytically inactive mutant of human APOBEC3B containing an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.DepositorInsertApolipoprotein mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal Doman E255A Catalytic Mutant (APOBEC3B Human)
UseCRISPRTagsExpressionMammalianMutationInsertion of an L1 intron into the coding sequenc…PromoterCMVAvailable sinceJuly 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pDS-Red-N1 GABAA beta3
Plasmid#109143PurposeThe expression vector carries the red fluorescent protein tagged at the C-terminus of beta3 subunit of the GABAa receptor.DepositorInsertGABAa receptor
UseTagsRFPExpressionMammalianMutationbeta3 subunit of the GABAa receptorPromoterCMVAvailable sinceJune 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBbS8k-RFP_R43W_E93G
Plasmid#109406PurposeRepA MutantDepositorInsertrepA
UseTagsRFPExpressionBacterialMutationR43W and E93G repA mutations to increase copy num…PromoterAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_YWHAH_14-3-3
Plasmid#109988PurposeProtein expression and purification of YWHAH_14-3-3DepositorInsertYWHAH_14-3-3 (YWHAH Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
1531_pAAV-U6-SA-Self1-gRNA-HLP-EmGFP-spA
Plasmid#109318PurposePlasmid for liver-specific expression of EmGFP with a gRNA against SaCas9DepositorInsertSaCas9 RuvC gRNA
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-X1-Neo-GFP-ATL2-deltaTM
Plasmid#109021Purposestable lentiviral expression of cDNADepositorInsertATL2-deltaTM (ATL2 Human)
UseLentiviralTagseGFPExpressionMammalianMutationtruncated form without C-terminal ER transmembran…PromoterEIF-1a promoterAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDM1516
Plasmid#109001Purposesafe haven knock-in plasmid for the act5 locus, C-terminal mCherry taggingDepositorTypeEmpty backboneUseSafe haven knock-in plasmid for the act5 locus, c…TagsmCherryExpressionMutationPromoterAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
1039 pBabe puroL PTEN A4
Plasmid#10941DepositorInsertPTEN (PTEN Human)
UseRetroviralTagsExpressionMammalianMutationA4: S380A, T382A, T383A, S385APromoterAvailable sinceJan. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
p3E-rab5aa
Plasmid#109716PurposeMultisite gateway vector for 3' tagging with rab5aaDepositorInsertrab5aa (rab5aa Zebrafish)
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-rab2a
Plasmid#109619PurposeMuscle specific zebrafish expression of mKate2 fused to rab2aDepositorInsertrab2a (rab2a Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
[pSK483] pLJC5 Flag-Depdc5(P)
Plasmid#109342PurposeLenti-viral expression of Flag-tagged Depdc5 mutant PDepositorInsertDepdc5 (DEPDC5 Human)
UseLentiviralTagsFLAGExpressionMutationYDLLP(775-779)GSGSGPromoterAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-EHD2b
Plasmid#109517PurposeMuscle specific zebrafish expression of mKate2 fused to EHD2bDepositorInsertEHD2b (ehd2 Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-Rac1
Plasmid#109529PurposeMuscle specific zebrafish expression of mKate2 fused to Rac1DepositorInsertRac1 (rac1a Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST-actc1b-ccdb-mKate2
Plasmid#109731PurposeMultisite gateway destination vector for muscle specific expression of constructs tagged at the 3' end with mKate2DepositorTypeEmpty backboneUseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST-actc1b-mKate2-ccdb
Plasmid#109732PurposeMultisite gateway destination vector for muscle specific expression of constructs tagged at the 5' end with mKate2DepositorTypeEmpty backboneUseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbS8k-RFP_R43W
Plasmid#109416PurposeRepA MutantDepositorInsertrepA
UseTagsRFPExpressionBacterialMutationR43W repA mutation to increase copy numberPromoterAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBD21_033
Plasmid#109150PurposepL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of SiriusDepositorInsertsmRFP
Sirius
UseSynthetic BiologyTagsExpressionBacterial and MammalianMutationPromoterCMV and pTetAvailable sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBD21_036
Plasmid#109153PurposepL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of mKO2DepositorInsertsmRFP
mKO2
UseSynthetic BiologyTagsExpressionBacterial and MammalianMutationPromoterCMV and pTetAvailable sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only