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Plasmid#54445DepositorInsertNuclear Export Signal (NES) of heat stable inhibitor (PKI)
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceJune 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET3a-AqAdk_V145C
Plasmid#18091DepositorInsertadenylate kinase (kad A. aeolicus)
UseTagsExpressionBacterialMutationVal 145 changed to CysPromoterAvailable sinceMay 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
C335
Plasmid#141125PurposeExpresses chimeric repressor (RbsR-L) under Cin promoterDepositorInsertsUseTagsssrA degradation tagExpressionBacterialMutationChimera: DNA binding domain of LacI and ligand bi…Promoterengineered cin promoter (responds to C14-HSL) and…Available sinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ybbR-HIS-Xyl-DocI
Plasmid#58711PurposeCharacteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tagDepositorInsertXyl-DocI
UseTagsHIS, HRV 3C, and ybbRExpressionBacterialMutationchanged Threonin 120 to Cysteine in Xylanase Doma…PromoterT7Available sinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
peT3a-AqAdk_MVGDH
Plasmid#18092DepositorInsertadenylate kinase (kad A. aeolicus)
UseTags6xHisExpressionBacterialMutationTyr 52 changed to Cys, Val 145 changed to Cys, …PromoterAvailable sinceMay 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ybbR-HIS-10FNIII(x4)-DocI
Plasmid#58712PurposeCharacteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tagDepositorInsert10FNIII(x4)-DocI
UseTagsHIS, HRV 3C, and ybbRExpressionBacterialMutationPromoterT7Available sinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ybbR-HIS-Spec(x2)-DocI
Plasmid#58713PurposeCharacteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tagDepositorInsertSpec(x2)-DocI
UseTagsHIS, HRV 3C, and ybbRExpressionBacterialMutationPromoterT7Available sinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
MTK Lentivirus Multi Cassette Destination Vector (pAN2424)
Plasmid#194221PurposeBackbone for constructing multi casette expression plasmids using the Mammalian TookitDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
GFP-noPS
Plasmid#177943PurposeTransient mammalian expression of eGFP. Lacks packaging sequence and is used as a negative control transfer plasmid for SARS-CoV-2 virus-like particles.DepositorInserteGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
Luc-noPS
Plasmid#177940PurposeTransient mammalian expression of Luc2 (firefly luciferase). Lacks packaging sequence and is used as a negative control transfer plasmid for SARS-CoV-2 virus-like particles.DepositorInsertLuc2
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
MTK Lentivirus Single Cassette Destination Vector (pAN2414)
Plasmid#194220PurposeBackbone for constructing single casette expression plasmids using the Mammalian TookitDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pROF051
Plasmid#155328PurposeConstitutive expression of SRDX-EL222 in plantsDepositorInsertPCaMV35S-SRDX-NLS-EL222-Tnos
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR_Gal4UASpyb-TATA_tBFP_pGK_mCitrine (RL407)
Plasmid#194217PurposeLentiviral transducition vector for a GAL4-responsive tagBFP reporter geneDepositorInsertUAS-ybTATA-tagBFP; PGK-mCitrine
UseLentiviralTagsExpressionMutationPromoterGAL4 UAS-ybTATA; PGKAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
LCB1_v1.3 (MTK 3a) (pZYW044)
Plasmid#194227PurposeMammalian Toolkit part 3a encoding the synthetic Spike binder LCB1DepositorInsertLCB1_v1.3
UseSynthetic BiologyTagsExpressionMutationPromoterNoneAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
LCB3_v1.2 (MTK 3a) (pZYW045)
Plasmid#194228PurposeMammalian Toolkit part 3a encoding the synthetic Spike binder LCB3DepositorInsertLCB3_v1.2
UseSynthetic BiologyTagsExpressionMutationPromoterNoneAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
WPRE (MTK 4b) (pAN2410)
Plasmid#194235PurposeMammalian Toolkit Part 4b encoding the WPRE for use with lentiviral backbonesDepositorInsertWPRE terminator
UseSynthetic BiologyTagsExpressionMutationPromoterNoneAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pROF018
Plasmid#155325PurposeConstitutive expression of KRAB-EL222 in plantsDepositorInsertPCaMV35S-NLS-KRAB-EL222-Tnos
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pROF050
Plasmid#155327PurposeConstitutive expression of BRD-EL222 in plantsDepositorInsertPCaMV35S-BRD-NLS-EL222-Tnos
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
RA35
Plasmid#141126PurposeExpresses dimeric LacI repressor (LacI-11) under Rhl promoterDepositorInsertsUseTagsssrA degradation tagExpressionBacterialMutationtruncated/dimeric version of LacI (missing last 1…Promoterengineered rhl promoter (responds to C4-HSL) and …Available sinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP-PS9
Plasmid#177944PurposeTransient mammalian expression of eGFP along with SARS-CoV-2 sequence 20080-21171 (PS9) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.DepositorInserteGFP (ORF1ab )
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only