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Plasmid#62979PurposeTranslational reporter for KLF4DepositorInsertKLF4 partial sequence (KLF4 Human)
UseLuciferaseTagsfirefly luciferaseExpressionMutationFragment 1-446 of KLF4PromoterCMVAvailable sinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA T7-Gnat1 Mut-3UTR
Plasmid#184027PurposeExpresses mouse Gnat1 protein with N-terminal T7 tag from cDNA that contains the mutant 3'-UTR where the TAG binding sites for MSI1 are mutated to TGADepositorInsertGnat1 (Gnat1 Mouse)
UseTagsT7ExpressionMammalianMutationTAG sites in the 3'-UTR are mutated to TGAPromoterCMVAvailable sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
Phage-PGK-Flag-HA-FKBP-T(WT)
Plasmid#181734Purposefor lentiviral expression of brachyury ORF in mammalian cellsDepositorInsertT (brachyury)
UseLentiviralTagsExpressionMutationa silent mutation in the PAM site of an sgRNAt ta…PromoterAvailable sinceMarch 21, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMIR-Report-Luc-KLF4-K2S
Plasmid#62980PurposeTranslational reporter for KLF4DepositorInsertKLF4 partial sequence (KLF4 Human)
UseLuciferaseTagsfirefly luciferaseExpressionMutationFragment 446-911 of KLF4PromoterCMVAvailable sinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMIR-Report-Luc-KLF4-K3S
Plasmid#62981PurposeTranslational reporter for KLF4DepositorInsertKLF4 partial sequence (KLF4 Human)
UseLuciferaseTagsfirefly luciferaseExpressionMutationFragment 911-1406 of KLF4PromoterCMVAvailable sinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMIR-Report-Luc-KLF4-K4S
Plasmid#62982PurposeTranslational reporter for KLF4DepositorInsertKLF4 partial sequence (KLF4 Human)
UseLuciferaseTagsfirefly luciferaseExpressionMutationFragment 1406-1781 of KLF4PromoterCMVAvailable sinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMIR-Report-Luc-KLF4-K6S
Plasmid#62983PurposeTranslational reporter for KLF4DepositorInsertKLF4 partial sequence (KLF4 Human)
UseLuciferaseTagsfirefly luciferaseExpressionMutationFragment 2086-2639 of KLF4PromoterCMVAvailable sinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2-Cx43-M100/125/147L-siResist
Plasmid#49856PurposeEncodes human connexin 43 with M100, M125, and M147 mutated to L and wobble mutations conferring resistance against an siRNADepositorInsertConnexin 43 (GJA1 Human)
UseTagsExpressionMammalianMutationMethionine100, Methionine 125, and Methionine 147…PromoterCMVAvailable sinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2-Cx43-M100/125L-siResist
Plasmid#49855PurposeEncodes human connexin 43 with M100 and M125 mutated to L and wobble mutations conferring resistance against an siRNADepositorInsertConnexin 43 (GJA1 Human)
UseTagsExpressionMammalianMutationMethionine100 and Methionine 125 mutated to Leuci…PromoterCMVAvailable sinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
Lenti-EFS-NTID194-GSQ-PMLivaWT-P2A-BLAST
Plasmid#208038PurposeEnables constitutive expression of N-terminal Split-TurboID-fused PML (isoform IVa; WT); to perform SUMO-ID; selection with blasticidinDepositorInsertPML (isoform IVa) (PML Human)
UseLentiviralTagsFLAG, N-terminal Split-TurboIDExpressionMutationPromoterEFSAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-EFS-BirAopt-GSQ-RNF4ca-P2A-BLAST
Plasmid#208047PurposeEnables constitutive expression of N-terminal BirAopt-fused RNF4ca (catalytically inactive RING mutant); to perform bioE3; selection with blasticidinDepositorInsertRNF4 (RNF4 Human)
UseLentiviralTagsBirAoptExpressionMutationCys>Ala mutations in conserved RING domainPromoterEFSAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMIR-Report-Luc-KLF4-K5S-Mt344A
Plasmid#34601DepositorInsertKLF4 (KLF4 Human)
UseLuciferaseTagsExpressionMammalianMutationThe KLF4 K5S region was mutated at the miR-344 bi…PromoterCMVAvailable sinceFeb. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2-Cx43-M100/125/147/213-siResist
Plasmid#49858PurposeEncodes human connexin 43 with M100, M125, M147, and M213 mutated to L and wobble mutations conferring resistance against an siRNADepositorInsertConnexin 43 (GJA1 Human)
UseTagsExpressionMammalianMutationMethionine100, Methionine 125, Methionine 147, an…PromoterCMVAvailable sinceDec. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMIR-Report-Luc-KLF4-K5S-Mt206B
Plasmid#34600DepositorInsertKLF4 (KLF4 Human)
UseLuciferaseTagsExpressionMammalianMutationThe KLF4 K5S region was mutated at the miR-206 bi…PromoterCMVAvailable sinceFeb. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
MKC84_HBB(HBA1reg-HBA1)
Plasmid#232410PurposeAAV production plasmid for HBA1 UTRs vector from Fig. 3 that mediates HDR at HBB locus using HBB sg7 gRNA. HBA gene includes HBA1 exons and introns; HBA1 UTRs flank HBA1 cassette.DepositorInsertHemoglobin Subunit Alpha 1 (HBA1 Human)
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceApril 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
MKC83_HBB(HBBreg-HBA1)
Plasmid#232409PurposeAAV production plasmid for HBB UTRs vector from Fig. 3 that mediates HDR at HBB locus using HBB sg7 gRNA. HBA gene includes HBA1 exons and introns; HBB UTRs flank HBA1 cassette.DepositorInsertHemoglobin Subunit Alpha 1 (HBA1 Human)
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceApril 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
Phage-PGK-Flag-HA-FKBP-T(G177D)
Plasmid#181735Purposefor lentiviral expression of brachyury ORF in mammalian cellsDepositorInsertT (brachyury)
UseLentiviralTagsExpressionMutationa silent mutation in the PAM site of an sgRNAt ta…PromoterAvailable sinceMarch 21, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMIR-Report-Luc-KLF4-K5S-Mt344B
Plasmid#34602DepositorInsertKLF4 (KLF4 Human)
UseLuciferaseTagsExpressionMammalianMutationThe KLF4 K5S region was mutated at the miR-344 bi…PromoterCMVAvailable sinceFeb. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMIR-Report-Luc-KLF4-K5S-Mt206A
Plasmid#34599DepositorInsertKLF4 (KLF4 Human)
UseLuciferaseTagsExpressionMammalianMutationThe KLF4 K5S region was mutated at the miR-206 bi…PromoterCMVAvailable sinceFeb. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
TRIPZ-bioGEF-UBnc-PURO
Plasmid#208044PurposeEnables inducible expression of low-affinity Avitag-UBnc; to perform bioE3; nc = non-cleavable mutation; selection with puromycinDepositorInsertUBnc (UBC Human)
UseLentiviralTagsLow-affinity AvitagExpressionMutationL73P Mutation near C-terminus suppresses cleavage…PromotertetO/UBCAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only