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We narrowed to 4,663 results for: crispr c plasmids

Showing: 321 - 340 of 4663 results

  2. CROP-seq-opti

    Plasmid
    #106280
    Purpose
    A version of the CROP-seq plasmid as presented in Datlinger et al. that contains the sgRNA-(F+E)-combined optimized backbone for CRISPRi from Chen et al.
    Depositor
    Jay Shendure
    Article
    Hill et al Nat Methods. 2018 Feb 19. pii: nmeth.460
    Insert
    EF1a-Puro-WPRE-hU6-gRNA
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    106280-plasmid-map-sequence-id-354474

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  3. pREDCas9

    Plasmid
    #71541
    Purpose
    Constitutive expression of Cas9, inducible expression of the Red recombineering system, and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.
    Depositor
    Tao Chen
    Article
    Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10
    Inserts
    Cas9
    lambda red genes
    plasmid curing system
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pBAD
    Available since
    July 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71541-plasmid-map-sequence-id-333602

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  4. CROPseq-Guide-Puro

    Plasmid
    #86708
    Purpose
    Makes CRISPR gRNAs detectable by single-cell RNA-seq. The gRNA is expressed as part of the puromycin resistance mRNA transcribed by RNA Pol II and in its functional form from the hU6 promoter.
    Depositor
    Christoph Bock
    Article
    Datlinger et al Nat Methods. 2017 Jan 18. doi: 10.1038/n
    Insert
    EF1a-Puro-WPRE-hU6-gRNA
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6
    Available since
    Feb. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    86708-plasmid-map-sequence-id-317051

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  5. pJC.15A.sgRNA.TA

    Plasmid
    #232483
    Purpose
    sgRNA cloning backbone for expression of sgRNA in C. difficile. Contains TA system to enable maintenence without antibiotic selection.
    Depositor
    James Collins
    Article
    Chua et al Microbiol Resour Announc. 2025 Apr 28:e0
    Inserts
    Toxin-antitoxin System from CD630
    medium-copy-number p15A origin of replication
    mCherry
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P3 constitutive promoter and native promoter
    Available since
    April 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    232483-plasmid-map-sequence-id-466415

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  18. pZNF57-donor

    Plasmid
    #112296
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF57 with GFP
    Depositor
    Kevin White
    Insert
    ZNF57 homology arms flanking EGFP-IRES-Neo cassette (ZNF57 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    rs2288959, rs2288958, rs10410539, hg38:19:2918211…
    Promoter
    Available since
    Dec. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    112296-plasmid-map-sequence-id-225584

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  19. pZNF565-donor

    Plasmid
    #112315
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF565 with GFP
    Depositor
    Kevin White
    Insert
    ZNF565 homology arms flanking EGFP-IRES-Neo cassette (ZNF565 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:19:36182553:A>T, hg38:19:36182595:A>G,…
    Promoter
    Available since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    112315-plasmid-map-sequence-id-228476

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  20. pNR1D2-donor

    Plasmid
    #112314
    Purpose
    CRISPR donor plasmid to tag human transcription factor NR1D2 with GFP
    Depositor
    Kevin White
    Insert
    NR1D2 homology arms flanking EGFP-IRES-Neo cassette (NR1D2 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:3:23977483:A>T, hg38:3:23977515:A>G, h…
    Promoter
    Available since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    112314-plasmid-map-sequence-id-228473

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Showing: 321 - 340 of 4663 results