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pMLS714

Plasmid

#188886

Purpose

MiniMos Transposon vector carrying Pmex-5::2xNLS::Cas9 and floxed Cbr-Unc-119

Depositor

Erik Jorgensen

Article

Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

Insert

Pmex-5::Cas9

Use

Tags

Expression

Worm

Mutation

C. elegans codom optimization and intron addition

Promoter

Available since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pMLS719

Plasmid

#188894

Purpose

miniMos Arm sgRNA expression vector

Depositor

Erik Jorgensen

Article

Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

Insert

PU6(K09B11.12)::miniMos Arm sgRNA

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-IT10

Plasmid

#66871

Purpose

Inntag 10: a small self-structured epitope aimed at innocuous protein tagging

Depositor

Marti Aldea

Article

Georgieva et al Nat Methods. 2015 Aug 31. doi: 10.1038/n

Insert

Inntag 10

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 27, 2015

Availability

Academic Institutions and Nonprofits only
pMLS234

Plasmid

#73682

Purpose

SapTrap 3-site Destination vector for N-terminal GFP tagging with embedded Cbr-unc-119 selectable marker

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

GFP + Cbr-unc-119

Use

CRISPR and Cre/Lox

Tags

GFP

Expression

Worm

Mutation

Promoter

Available since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCFJ1258

Plasmid

#44478

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1, [4-3] Gateway

Use

Gateway destination vector

Tags

Expression

Mutation

Promoter

Available since

April 25, 2013

Availability

Academic Institutions and Nonprofits only

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pCFJ1666

Plasmid

#51485

Purpose

miniMos transposable element with PuroR, MCS cloning

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Type

Empty backbone

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

March 3, 2014

Availability

Academic Institutions and Nonprofits only

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pGlab592

Plasmid

#236554

Purpose

240 basepair upstream sequence of KSHV ORF68 driving HaloTag expression

Depositor

Britt Glaunsinger

Article

Morgens et al Mol Syst Biol. 2025 Jan;21(1):30-58. doi

Insert

KSHV ORF68 upstream 240bp

Use

Lentiviral

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

Available since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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pMLS1272

Plasmid

#188891

Purpose

Ric-4 sgRNA expression vector

Depositor

Erik Jorgensen

Article

Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

Insert

PU6(R07E5.16)::Ric-4 sgRNA (CELE_Y22F5A.3 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pET-11-LECT2-V40

Plasmid

#183031

Purpose

Expression of LECT2 V40 variant protein from T7 promoter

Depositor

Gareth Morgan

Article

Belonogov et al bioRxiv 2022.03.01.482540

Insert

LECT2 (LECT2 Human)

Use

Tags

Expression

Bacterial

Mutation

V40 variant

Promoter

T7

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pRS416-PTEF1-MCP-3P-ECM33_intron

Plasmid

#127601

Purpose

low-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, 3P-ECM33 intron inserted into URA3

Depositor

David Bartel

Article

Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

Insert

ECM33 intron with MS2 hairpins inserted near 3′ end of intron

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRS416-PTEF1-MCP-5P-ECM33_intron

Plasmid

#127599

Purpose

low-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, 5P-ECM33 intron inserted into URA3

Depositor

David Bartel

Article

Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

Insert

ECM33 intron with MS2 hairpins inserted near 5′ end of intron

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRS416-PTEF1-MCP-ECM33_intron

Plasmid

#127598

Purpose

low-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, ECM33 intron inserted into URA3

Depositor

David Bartel

Article

Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

Insert

ECM33 intron

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 9, 2020

Availability

Academic Institutions and Nonprofits only

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pMLS415

Plasmid

#114272

Purpose

SapTrap donor vector for point mutagenesis; pENTR-221::syntron(lox2272-119+)

Depositor

Erik Jorgensen

Article

Schwartz et al MicroPubl Biol. 2018 Jul 29;2018:10.1791

Insert

Cbr-Unc-119

Use

CRISPR and Cre/Lox

Tags

Expression

Mutation

*please see depositor comment below

Promoter

Available since

Dec. 17, 2018

Availability

Academic Institutions and Nonprofits only

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p415TEF cyto roGFP2-Grx2(yeast)

Plasmid

#65005

Purpose

Yeast expression plasmid for roGFP2-Grx2; yeast codon optimized roGFP; yeast Grx2

Depositor

Tobias Dick

Article

Morgan et al Nat Chem Biol. 2013 Feb;9(2):119-25. doi

Insert

GRX2

Use

Tags

roGFP2

Expression

Yeast

Mutation

Promoter

Available since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV CEPIA2mt

Plasmid

#58218

Purpose

Green fluorescent indicator for calcium imaging in the mitochondria

Depositor

Masamitsu Iino

Article

Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

Insert

CEPIA2mt

Use

Tags

myc

Expression

Mammalian

Mutation

GCaMP2 N263Y E282V M339L

Promoter

CMV

Available since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

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pLIX403-hNOS2

Plasmid

#110800

Purpose

tet-inducible NOS2 expression

Depositor

Edward Morgan

Article

Lee et al Free Radic Biol Med. 2017 Jul;108:478-48

Insert

NOS2 (NOS2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.(Cyto)iGlucoSnFR

Plasmid

#164513

Purpose

Green glucose sensor, cytosolic

Depositor

Loren Looger

Article

Keller et al bioRxiv (2019) 571422; doi: https://doi.

Insert

(Cyto)iGlucoSnFR

Use

AAV

Tags

Expression

Mutation

Synthetic Construct

Promoter

GFAP

Available since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.(Cyto)iGlucoSnFR.mRuby2

Plasmid

#164514

Purpose

Green glucose sensor with mRuby2 fusion, cytosolic

Depositor

Loren Looger

Article

Keller et al bioRxiv (2019) 571422; doi: https://doi.

Insert

(Cyto)iGlucoSnFR-mRuby2

Use

AAV

Tags

Expression

Mutation

Synthetic Construct

Promoter

GFAP

Available since

Feb. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pCIS GEM-CEPIA1er

Plasmid

#58217

Purpose

Ratiometric (Blue to Green) fluorescent indicator for calcium signaling in the endoplasmic reticulum

Depositor

Masamitsu Iino

Article

Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

Insert

GEM-CEPIA1er

Use

Tags

myc

Expression

Mammalian

Mutation

GEM-GECO1 E298D F359W D400E E407D

Promoter

CAG

Available since

July 28, 2014

Availability

Academic Institutions and Nonprofits only

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pJK02

Plasmid

#105133

Purpose

Clostridium difficile CRISPR-cas9 mutagenesis plasmid traJ oriT

Depositor

Joseph Sorg

Article

McAllister et al Sci Rep. 2017 Nov 7;7(1):14672. doi: 10.

Inserts

Upstream & Downstream pyrE deletion region
tetR
Cas9
gRNA

Use

E. coli - c. difficile shuttle vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

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