We narrowed to 2,605 results for: ubiquitin

1. Halo-Tev-Flag-Ube2v1

   Plasmid

   #110069

   Purpose

   Express Halo-Tev-Flag-Ube2v1 (human) in mammalian cells

   Depositor

   Yimon Aye

   Article

   Zhao et al ACS Cent Sci. 2018 Feb 28;4(2):246-259.

   Insert

   Halo-Tev-Flag-Ube2v1 (UBE2V1 Human)
   

   Use

   Tags

   Halo-TEV-Flag

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. 908H

   Plasmid

   #160240

   Purpose

   TetO(2x)-Hsp70min-UASpmin-Firefly-T2A-eGFP-p10-3’UTR-Ubiquitin-Renilla-attB

   Depositor

   Omar Akbari

   Article

   Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

   Inserts

   Tet Operator (2x)
   hsp70 minimal promoter
   p-element promoter
   Firefly luciferase
   Renilla luciferase
   Ubiquitin promoter
   

   Use

   Tags

   T2A GFP

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Nov. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. 908E

   Plasmid

   #160238

   Purpose

   TtgO(2x)-Hsp70min-UASpmin-Firefly-T2A-eGFP-p10-3’UTR-Ubiquitin-Renilla-attB

   Depositor

   Omar Akbari

   Article

   Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

   Inserts

   Ttg Operator (2x)
   hsp70 minimal promoter
   p-element promoter
   Firefly luciferase
   Renilla luciferase
   Ubiquitin promoter
   

   Use

   Tags

   T2A GFP

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Nov. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. 908G

   Plasmid

   #160239

   Purpose

   PipO(3x)-Hsp70min-UASpmin-Firefly-T2A-eGFP-p10-3’UTR-Ubiquitin-Renilla-attB

   Depositor

   Omar Akbari

   Article

   Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

   Inserts

   Pip Operator (3x)
   hsp70 minimal promoter
   p-element promoter
   Firefly luciferase
   Renilla luciferase
   Ubiquitin promoter
   

   Use

   Tags

   T2A GFP

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Nov. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. 908J

   Plasmid

   #160242

   Purpose

   CymO(2x)-Hsp70min-UASpmin-Firefly-T2A-eGFP-p10-3’UTR-Ubiquitin-Renilla-attB

   Depositor

   Omar Akbari

   Article

   Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

   Inserts

   Cym Operator (2x)
   hsp70 minimal promoter
   p-element promoter
   Firefly luciferase
   Renilla luciferase
   Ubiquitin promoter
   

   Use

   Tags

   T2A GFP

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Nov. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. 908I

   Plasmid

   #160241

   Purpose

   VanO(2x)-Hsp70min-UASpmin-Firefly-T2A-eGFP-p10-3’UTR-SV40-Ubiquitin-Renilla-attB

   Depositor

   Omar Akbari

   Article

   Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

   Inserts

   Van Operator (2x)
   hsp70 minimal promoter
   p-element promoter
   Firefly luciferase
   Renilla luciferase
   Ubiquitin promoter
   

   Use

   Tags

   T2A GFP

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Nov. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCAG-BRCA1-RNF169UBD

   Plasmid

   #119009

   Purpose

   Mammalian expression of a fusion protein of human BRCA1 with the human RNF169 Ubiquitin binding domain and BFP expression

   Depositor

   Ralf Kuehn

   Article

   Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

   Insert

   BRCA1 (BRCA1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-BRCA1-Rad18UBD

   Plasmid

   #119008

   Purpose

   Mammalian expression of a fusion protein of human BRCA1 with human Rad18 Ubiquitin binding domain and BFP expression

   Depositor

   Ralf Kuehn

   Article

   Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

   Insert

   BRCA1 (BRCA1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. His8:MBP-tev-Ala-eK-Flv

   Plasmid

   #83393

   Purpose

   bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies

   Depositor

   Nico Dissmeyer

   Article

   Mot et al New Phytol. 2017 Mar 9. doi: 10.1111/nph

   Insert

   flavin binding protein MioC
   

   Use

   Synthetic Biology

   Tags

   3xHA, 8xHis, and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. His8:MBP-tev-Ile-eK-Flv

    Plasmid

    #83392

    Purpose

    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies

    Depositor

    Nico Dissmeyer

    Article

    Mot et al New Phytol. 2017 Mar 9. doi: 10.1111/nph

    Insert

    flavin binding protein MioC
    

    Use

    Synthetic Biology

    Tags

    3xHA, 8xHis, and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. His8:MBP-tev-Tyr-eK-Flv

    Plasmid

    #83391

    Purpose

    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies

    Depositor

    Nico Dissmeyer

    Article

    Mot et al New Phytol. 2017 Mar 9. doi: 10.1111/nph

    Insert

    flavin binding protein MioC
    

    Use

    Synthetic Biology

    Tags

    3xHA, 8xHis, and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pZMU-Dest

    Plasmid

    #105089

    Purpose

    Yeast expression vector for Gateway cloning and SUS bridge assays

    Depositor

    Christopher Grefen

    Article

    Grefen et al Methods Mol Biol. 2014;1062:659-78. doi:

    Type

    Empty backbone

    Use

    Tags

    myc

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCR3-FLAG-Ubc13-WT

    Plasmid

    #43938

    Depositor

    Bryant Darnay

    Article

    Lamothe et al J Biol Chem. 2007 Feb 9;282(6):4102-12.

    Insert

    ubiquitin-conjugating enzyme E2N (UBE2N Human)
    

    Use

    Tags

    2x FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMV 3xFLAG-ATG3 WT

    Plasmid

    #129296

    Purpose

    Expresses 3xFLAG-ATG3 WT in mammalian cells

    Depositor

    Robin Ketteler

    Article

    Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

    Insert

    Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCR3-FLAG-Ubc13-C87A

    Plasmid

    #43939

    Depositor

    Bryant Darnay

    Article

    Lamothe et al J Biol Chem. 2007 Feb 9;282(6):4102-12.

    Insert

    ubiquitin-conjugating enzyme E2N (UBE2N Human)
    

    Use

    Tags

    2x FLAG

    Expression

    Mammalian

    Mutation

    C87A

    Promoter

    Available since

    Nov. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pENTR221 UBR5 ∆PABC

    Plasmid

    #81064

    Purpose

    Gateway entry vector encoding full length UBR5 Y2402A/K2415A (∆PABC)

    Depositor

    Darren Saunders

    Article

    Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

    Insert

    Ubiquitin protein ligase E3 component N-recognin 5 (UBR5) ∆PABC (UBR5 Human)
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    Y2402A and K2415A (bp t7204g, a7205c, t7206c, a72…

    Promoter

    N/A

    Available since

    Jan. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDONR223 ATG3 WT

    Plasmid

    #129292

    Purpose

    Gateway entry clone encoding wild-type human ATG3 with a stop codon

    Depositor

    Robin Ketteler

    Article

    Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

    Insert

    Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
    

    Use

    Gateway entry vector / entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAIP4_2A4D

    Plasmid

    #234196

    Purpose

    Heterologous protein expression of ubiquitin-conjugating enzyme E2 variant 1 (UEV-1) (CROC-1) in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    2A4D (UBE2V1 Human)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pVP16-tev-PRT1-C195A

    Plasmid

    #83408

    Purpose

    bacterial expression vector pVP16 containing a mutant variant of Arabidopsis thaliana PROTEOLYSIS 1 (PRT1) N-end rule E3 ubiquitin ligase to be used in N-end rule in vitro ubiquitination studies

    Depositor

    Nico Dissmeyer

    Insert

    PROTEOLYSIS1 (PRT1) (PRT1 Mustard Weed, E. coli)
    

    Use

    Tags

    His8, MBP, and tev cleavage site

    Expression

    Bacterial

    Mutation

    C195A substitution

    Promoter

    Available since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pVP16-tev-PRT1-C29A

    Plasmid

    #83407

    Purpose

    bacterial expression vector pVP16 containing a mutant variant of Arabidopsis thaliana PROTEOLYSIS 1 (PRT1) N-end rule E3 ubiquitin ligase to be used in N-end rule in vitro ubiquitination studies

    Depositor

    Nico Dissmeyer

    Insert

    PROTEOLYSIS1 (PRT1) (PRT1 Mustard Weed, E. coli)
    

    Use

    Tags

    His8, MBP, and tev cleavage site

    Expression

    Bacterial

    Mutation

    C29A substitution

    Promoter

    Available since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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