We narrowed to 12,482 results for: BASE

1. Cenp-B INCENP GFP

   Plasmid

   #45238

   Purpose

   Target INCENP to centromeres via full length CENP-B

   Depositor

   Michael Lampson

   Article

   Wang et al J Cell Biol. 2011 Aug 22;194(4):539-49.

   Insert

   Cenp-B, INCENP-Δcen, GFP
   

   Use

   Tags

   GFP and full length CENP-B

   Expression

   Mammalian

   Mutation

   CEN box deleted from INCENP (aa1-46)

   Promoter

   Available since

   Sept. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKEB31

   Plasmid

   #31224

   Depositor

   Adam Bogdanove, Daniel Voytas

   Article

   Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

   Insert

   ccdB and Cm resistance gene
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 30, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMB-TMEr

   Plasmid

   #61208

   Purpose

   Fluorescent reporter for plasma membrane and peri-arbuscular membrane MtBCPsp-mCherry-BCP expressed under MtBCP1 promoter

   Depositor

   Maria Harrison

   Article

   Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

   Insert

   MtBCPsp-mCherry-BCP
   

   Use

   Tags

   mCherry

   Expression

   Plant

   Mutation

   Promoter

   MtBCP1

   Available since

   April 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. eGFP L138 gRNA

   Plasmid

   #119133

   Purpose

   gRNA that guides Cas9 and APOBEC complexes to L138 in eGFP.

   Depositor

   Reuben Harris

   Article

   Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

   Insert

   eGFP L138 gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   None

   Promoter

   U6

   Available since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. qTAG-C-3xHA-Zeo

   Plasmid

   #207740

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xHA-2A-Zeo cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. qTAG-N-Zeo-3xHA

   Plasmid

   #207707

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-3xHA cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pDM067

   Plasmid

   #216820

   Purpose

   Aspergillus nidulans codon-adjusted mStayGold2 fluorescent protein, includes linker for C-terminal tagging.

   Depositor

   Ken Sawin

   Article

   Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

   Insert

   mStayGold2
   

   Use

   Tags

   VinnyLinker

   Expression

   Bacterial

   Mutation

   Aspergillus nidulans codon-adjusted

   Promoter

   Available since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. apt-CBE-3'end

   Plasmid

   #219939

   Purpose

   Mammalian expression of apt-CBE-3'end protein (MCP_32aa-linker_evoAPOBEC1) with P2A-EGFP marker

   Depositor

   Alexis Komor

   Article

   Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

   Insert

   MCP_32-aa_evo-rA1_2xUGI
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBJ5

   Plasmid

   #167134

   Purpose

   A gentamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pfrr promoter

   Depositor

   Akira Mine

   Article

   Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d

   Insert

   luxCDABE
   

   Use

   Luciferase

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Frr promoter

   Available since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. nCas9-SpRY_P2A-mCherry

    Plasmid

    #219935

    Purpose

    Mammalian expression of SpRY-nCas9 with P2A-mCherry marker for use with MOBE systems

    Depositor

    Alexis Komor

    Article

    Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

    Insert

    Sp-nCas9(D10A)-SpRY_P2A-mCherry
    

    Use

    CRISPR

    Tags

    SV40 bpNLS and SV40 bpNLS-P2A-mCherry

    Expression

    Mammalian

    Mutation

    nCas9(D10A)

    Promoter

    CMV

    Available since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pC0091

    Plasmid

    #181955

    Purpose

    Beta catenin reporter control M51 Super 8x (mutated TCF/LEF binding sites) FOPFlash with Gluc/Cluc

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Science. 2019 Jul 11. pii: science.aax70

    Insert

    7xFOP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNeoXTR f1

    Plasmid

    #69158

    Purpose

    mouse targeting vector

    Depositor

    David Feldser

    Article

    Robles-Oteiza et al Nat Commun. 2015 Nov 5;6:8783. doi: 10.1

    Inserts

    DTA
    NeoR
    GFP
    AmpR
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPRIME-CMV-dsRed-recipient

    Plasmid

    #11658

    Purpose

    3rd generation lentiviral transfer vector. pPRIME-mating recipient plasmid with CMV promoter controlling dsRed expression

    Depositor

    Stephen Elledge

    Article

    Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    dsRed

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 1, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMK451 (BSD-P2A-dTAG)

    Plasmid

    #214381

    Purpose

    dTAG for N-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    BSD-P2A-dTAG
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pXL-BacII-CAG-loxP-STOP-loxP-Venus

    Plasmid

    #170520

    Purpose

    Plasmid containing piggyBac ITRs flanking CAG, a loxP-STOP-loxP cassette and Venus (also see doi: 10.7554/eLife.63907 )

    Depositor

    Joshua Sanes

    Article

    Yamagata et al Bio Protoc. 2021 Aug 5;11(15):e4105. doi

    Insert

    CAG-loxP-STOP-loxP-Venus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pNeoXTR f2

    Plasmid

    #69159

    Purpose

    mouse targeting vector

    Depositor

    David Feldser

    Article

    Robles-Oteiza et al Nat Commun. 2015 Nov 5;6:8783. doi: 10.1

    Inserts

    DTA
    NeoR
    GFP
    AmpR
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV-Synapsin-mCherry-C1-WPRE

    Plasmid

    #159956

    Purpose

    untargeted marker

    Depositor

    Michael Courtney

    Article

    Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

    Insert

    mCherry-C1
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    synthetic

    Promoter

    hSyn1

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMH5

    Plasmid

    #52531

    Purpose

    expresses FLP recombinase in Drosophila

    Depositor

    Klaus Foerstemann

    Article

    Bottcher et al Nucleic Acids Res. 2014 Apr 19.

    Insert

    FLP recombinase (flp Budding Yeast)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    ubiquitin

    Available since

    April 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-T7-ABE8.20m-nSpCas9-VRQR-P2A-EGFP (KAC1166)

    Plasmid

    #185918

    Purpose

    pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9-VRQR(D10A/D1135V/G1218R/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Insert

    human codon optimized ABE8.20m-nSpCas9-VRQR-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9-VRQR=D10A/D1135V/G1218R/R1335Q/T1337R

    Promoter

    CMV and T7

    Available since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEF1α-Cre-2A-miRFP670nano3

    Plasmid

    #207747

    Purpose

    Expression of the Cre recombinase linked to a miRFP670nano3 fluorescent marker

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    Cre-PGK-miRFP670nano3
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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