We narrowed to 27,367 results for: STI
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Plasmid#208974PurposeRecruited B103 DNA polymerase with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.DepositorInsertB103-BPNLS-CC(N5)
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianPromoterCMV and T7Available SinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
Recruited polymerase expression - pCMV-T7-Phi29-CC(N5) (LM2726)
Plasmid#208971PurposeRecruited Phi29 DNA polymerase (-exo) with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.DepositorInsertPhi29-BPNLS-CC(N5)
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationPhi29(-exo;D169A)PromoterCMV and T7Available SinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
Recruited polymerase expression - pCMV-T7-Phi29(+exo)-CC(N5) (LM2761)
Plasmid#208970PurposeRecruited Phi29(+exo) DNA polymerase with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.DepositorInsertPhi29(+exo)-BPNLS-CC(N5)
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianPromoterCMV and T7Available SinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR4 Ta-sro1 WWE-PARP
Plasmid#192548PurposepENTR plasmid Triticum aestivum sro1, amino acids 1-434, cultivar SR3, no stop codonDepositorInsertSIMILAR TO RCD ONE 1 (SRO1) WWE-PARP domains, cultivar Shanrong No. 3 (SR3)
UseGateway entry vectorAvailable SinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOPINF Ta-sro1 PARP
Plasmid#192549PurposeE. coli expression vector for Triticum aestivum sro1 PARP domain (amino acids 246-434)DepositorInsertSIMILAR TO RCD ONE 1 (SRO1) PARP domain, cultivar Shanrong No. 3 (SR3)
TagsHis6, 3C protease cleavage siteExpressionBacterial, Insect, and Mamm…PromoterT7 promoterAvailable SinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
p1A Prk-
Plasmid#162694Purposep1A negative control expressing CbbLS and inactive Prk (Prk K20M S21A)DepositorInsertH. neapolitanus CbbLS and S. elongatus Prk
TagsN terminal 6x His tag on PRKExpressionBacterialMutationPrk inactive (K20M S21A native protein, this map …PromoterPLtet0-1 promoterAvailable SinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
289aa Met133, 138, 186Ileu ELL2
Plasmid#127272PurposeExpresses 289aa human ELL2 with Met133, 138, 186IleuDepositorInsertELL2 w Met to ILeus stopped at XbaI (ELL2 Human)
ExpressionMammalianMutationMet133, 138, 186 Ileu, cut with XbaI blunt fillinPromoterpEF1aAvailable SinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
frameshift ELL2
Plasmid#127257PurposeFor in vitro translation of human a frameshift ELL2 that results in a 58 kDa product from internal initiation.DepositorInsertELL2 (ELL2 Human)
UseIn vitro translationMutationXhoI site between the 1st &2nd Met was filled…PromoterT7Available SinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
564aa ELL2 bluescript
Plasmid#127267PurposeFor in vitro translation of human ELL2 lacking COOH regionDepositorInsertELL2 (ELL2 Human)
UseIn vitro translationMutationAddgene Plasmid 127256--SphI site filled in, then…PromoterT7Available SinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLX304 PCSK5 (H516P)-V5 blast
Plasmid#83103PurposeLentiviral vector for constitutive expression of human mutant PC5A (H516P) with C-terminal V5 tagDepositorInsertPCSK5 (PCSK5 Human)
UseLentiviralTagsV5ExpressionMammalianMutationChanged Histidine 516 to ProlineAvailable SinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
csd6_17-330/pET28b(+) N-tag
Plasmid#71353Purposecsd6, construct:17-330, pET28b(+) N-tag (Please note that this plasmid has a C-terminal His tag)DepositorAvailable SinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
csd6_13-330_E110A/pET28b(+) N-tag
Plasmid#71354Purposecsd6, construct:13-330, pET28b(+) N-tag, mutation:E110ADepositorInsertHP0518 (HP0518 changed Glutamic acid 110 to Alanine)
TagsHistidine tagExpressionBacterialPromoterT7Available SinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
csd6_13-330_Y133A/pET28b(+) N-tag
Plasmid#71356Purposecsd6, construct:13-330, pET28b(+) N-tag, mutation:Y133ADepositorInsertHP0518 (HP0518 changed Tyrosine 133 to Alanine)
TagsHistidine tagExpressionBacterialPromoterT7Available SinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
csd6_13-330_C176A/pET28b(+) N-tag
Plasmid#71360Purposecsd6, construct:13-330, pET28b(+) N-tag, mutation:C176ADepositorInsertHP0518 (HP0518 changed Cysteine 176 to Alanine)
TagsHistidine tagExpressionBacterialPromoterT7Available SinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
csd6_13-330_W158A/pET28b(+) N-tag
Plasmid#71358Purposecsd6, construct:13-330, pET28b(+) N-tag, mutation:W158ADepositorInsertHP0518 (HP0518 changed Tryptophan 158 to Alanine)
TagsHistidine tagExpressionBacterialPromoterT7Available SinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
csd6_13-330_Y132A/pET28b(+) N-tag
Plasmid#71355Purposecsd6, construct:13-330, pET28b(+) N-tag, mutation:Y132ADepositorInsertHP0518 (HP0518 changed Tyrosine 132 to Alanine)
TagsHistidine tagExpressionBacterialPromoterT7Available SinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBullet-cyt-n
Plasmid#53065Purposedestination vector with cytosol marker (ECFP) for tagged fluorescent protein (N-ter-EYFP) of plant geneDepositorTypeEmpty backboneUseSynthetic BiologyTagsECFP and EYFPAvailable SinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBullet-er-n
Plasmid#53067Purposedestination vector with WAK2 (29 aa)-ECFP- ER marker (HDEL) for tagged fluorescent protein (N-ter-EYFP) of plant geneDepositorTypeEmpty backboneUseSynthetic BiologyTagsECFP and EYFPAvailable SinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBullet-tgn-n
Plasmid#53071Purposedestination vector with ECFP-trans golgi network marker (VTI12) for tagged fluorescent protein (N-ter-EYFP) of plant geneDepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
Click editor (CE1) - pCMV-T7-PCV2-nCas9-EcKlenow (EMK488)
Plasmid#208942PurposeThe CE1 construct, expressed from CMV or T7 promoters.DepositorInsertPCV2-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationnSpCas9(H840A); EcKlenow(-exo;D355A/E357A)PromoterCMV and T7Available SinceNov. 22, 2023AvailabilityAcademic Institutions and Nonprofits only