We narrowed to 5,563 results for: cycs

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pLG012 (DRT type 4)

Plasmid

#157890

Purpose

Expresses the DRT type 4 defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

DRT type 4

Expression

Bacterial

Available Since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pLG013 (DRT type 5)

Plasmid

#157891

Purpose

Expresses the DRT type 5 defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

DRT type 5

Expression

Bacterial

Available Since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pRPF185

Plasmid

#106367

Purpose

C. difficile inducible expression system. Shuttle plasmid containing a tetracycline-inducible gusA

Depositor

Robert Fagan, Neil Fairweather

Article

Fagan et al J Biol Chem. 2011 Aug 5;286(31):27483-93

Insert

Term(fdx)-Ptet-gusA-Term(slpA) (gusA )

Use

E. coli - c. difficile shuttle vector

Mutation

codon optimised for C. difficile

Promoter

Ptet

Available Since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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pQFT-mNGsacB

Plasmid

#203676

Purpose

pQFT with mNeonGreen and sacB under control of PQJ

Depositor

Urs Jenal

Article

Klotz et al Appl Environ Microbiol. 2023 May 18:e002

Inserts

mNeonGreen
sacB

Expression

Bacterial

Mutation

Has synonymous (silent) mutations to remove BsrGI…

Promoter

PQJ

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pNO286-3

Plasmid

#107746

Purpose

Key genes: "mini-CcaS #10", ho1, pcyA, spectinomycin resistance, and medium copy backbone (p15a)

Depositor

Jeffrey Tabor

Article

Ong et al Chembiochem. 2018 Feb 8. doi: 10.1002/cb

Inserts

CcaS
Ho1
PcyA

Use

Synthetic Biology

Expression

Bacterial

Mutation

Deleted AA 223 and 512

Promoter

J23106 and J23108

Available Since

April 26, 2018

Availability

Academic Institutions and Nonprofits only

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pWJ66

Plasmid

#46570

Purpose

Same as pdCas9, but with the dCas9-w fusion (w is fused at the C-terminal end of dCas9)

Depositor

Luciano Marraffini

Article

Bikard et al Nucleic Acids Res. 2013 Jun 12.

Inserts

tracrRNA
dcas9-w
CRISPR array

Use

CRISPR

Tags

rpoZ

Expression

Bacterial

Mutation

D10A, H840A

Available Since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only

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pSW002

Plasmid

#111248

Purpose

Broad host-range bacterial vector

Depositor

Rosemarie Wilton

Article

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Type

Empty backbone

Expression

Bacterial

Available Since

July 12, 2018

Availability

Academic Institutions and Nonprofits only

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pSR43.6r

Plasmid

#63197

Purpose

Expresses CcaS, Ho1, and PcyA constitutively

Depositor

Jeffrey Tabor

Article

Schmidl et al ACS Synth Biol. 2014 Nov 21;3(11):820-31

Inserts

CcaS
Ho1
PcyA

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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LV-TRE-VP64 mouse MyoD-T2A-dsRedExpress2

Plasmid

#60625

Purpose

Expresses VP64 mouse MyoD and dsRedExpress2 in response to doxycycline

Depositor

Charles Gersbach

Article

Kabadi et al ACS Synth Biol. 2014 Dec 10.

Insert

VP64 mouse MyoD

Use

Lentiviral

Promoter

TRE

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pSR33.4r

Plasmid

#63198

Purpose

Expresses Cph8, Ho1, and PcyA constitutively

Depositor

Jeffrey Tabor

Article

Schmidl et al ACS Synth Biol. 2014 Nov 21;3(11):820-31

Inserts

Cph8
Ho1
PcyA

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 16, 2015

Availability

Academic Institutions and Nonprofits only

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rTetR-GFP

Plasmid

#183916

Purpose

Reverse Tet repressor labeled with GFP; binds tetO sites upon doxycycline addition

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

rTetR-GFP

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pLG004 (RT-Abi-P2)

Plasmid

#157882

Purpose

Expresses the RT-Abi-P2 defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

RT-Abi-P2

Expression

Bacterial

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-DEST47-SIRT7

Plasmid

#242137

Purpose

Expression in mammalian cells

Depositor

J. Carl Barrett, Izumi Horikawa

Article

Michishita et al Mol Biol Cell. 2005 Oct;16(10):4623-35.

Insert

SIRT7 (SIRT7 Human)

Tags

Cycle 3 GFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pSLIK-rABE-Flag

Plasmid

#191383

Purpose

Doxycycline inducible expression of RNA A-to-I editor rABE

Depositor

Stephen Floor

Article

Lin et al Nat Methods. 2023 Dec;20(12):1887-1899.

Insert

rABE-Flag

Use

Lentiviral and Synthetic Biology

Tags

Flag

Expression

Mammalian

Promoter

tet-inducible promoter

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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TLCV2-ApaLI-FLAG-T2A-mTagBFP2-Puro

Plasmid

#211462

Purpose

Lentivirus backbone expressing a doxycycline-inducible mitochondrial localized ApaLI

Depositor

Agnel Sfeir

Article

Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

Insert

ApaLI

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

TRE

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLG014 (RADAR)

Plasmid

#157892

Purpose

Expresses the RADAR defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

RADAR

Expression

Bacterial

Available Since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pLG015 (RADAR)

Plasmid

#157893

Purpose

Expresses the RADAR defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

RADAR subtype containing auxillary gene rdrD

Expression

Bacterial

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pQE60-RRF-His

Plasmid

#124132

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Ribosome recycling factor (frr Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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PB-CA-rtTA Adv

Plasmid

#20910

Purpose

piggyBac vector for expression of reverse tetracycline transactivator from a CAG constitutive promoter

Depositor

Andras Nagy

Article

Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi

Insert

rtTA-ON-Adv

Use

piggybac

Expression

Mammalian

Available Since

April 23, 2009

Availability

Academic Institutions and Nonprofits only

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pACλCI-β-flap (831-1057)

Plasmid

#53733

Purpose

Expression of λCI fused via three alanines to E. coli RNAP beta subunit, aa 831-1057 (β-flap). Can be cut with Not1 and BamHI to replace β-flap with a moiety of interest.

Depositor

Ann Hochschild

Article

Dove et al Nature. 1997 Apr 10;386(6625):627-30.

Insert

λCI-β-flap fusion

Expression

Bacterial

Promoter

lac UV5

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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