We narrowed to 4,430 results for: Erf

1. pMM729

   Plasmid

   #133598

   Purpose

   Part Plasmid for Mxi1-dCas9, Part 3(coding sequence)

   Depositor

   Megan McClean

   Article

   Geller et al Cell Mol Bioeng. 2019 Sep 24;12(5):511-5

   Insert

   Mxi1-dCas9
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMM767

   Plasmid

   #133604

   Purpose

   Part Plasmid for dCas9 NLS, Part 3(coding sequence)

   Depositor

   Megan McClean

   Article

   Geller et al Cell Mol Bioeng. 2019 Sep 24;12(5):511-5

   Insert

   dCas9 NLS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMM585

   Plasmid

   #133591

   Purpose

   Part Plasmid for dCas9, Part 3a(coding sequence)

   Depositor

   Megan McClean

   Article

   Geller et al Cell Mol Bioeng. 2019 Sep 24;12(5):511-5

   Insert

   dCas9
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMM766

   Plasmid

   #133603

   Purpose

   Part Plasmid for dCas9 NLS Stop, Part 3b(coding sequence)

   Depositor

   Megan McClean

   Article

   Geller et al Cell Mol Bioeng. 2019 Sep 24;12(5):511-5

   Insert

   dCas9 NLS Stop
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMM768

   Plasmid

   #133605

   Purpose

   Part Plasmid for dCas9 NLS, Part 3b(coding sequence)

   Depositor

   Megan McClean

   Article

   Geller et al Cell Mol Bioeng. 2019 Sep 24;12(5):511-5

   Insert

   dCas9 NLS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMM769

   Plasmid

   #133606

   Purpose

   Part Plasmid for dCas9 NLS, Part 3a(coding sequence)

   Depositor

   Megan McClean

   Article

   Geller et al Cell Mol Bioeng. 2019 Sep 24;12(5):511-5

   Insert

   dCas9 NLS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pVHed07

   Plasmid

   #82350

   Purpose

   Bxb1/TP901 temporal logic gate with mKate2 and sfGFP in Phi80 chromosomal integration plasmid

   Depositor

   Richard Murray

   Article

   Hsiao et al Mol Syst Biol. 2016 May 17;12(5):869. do

   Inserts

   mKate2 RFP
   Superfolder GFP
   Temporal logic gate with TP901 and Bxb1 attachment sites
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJBL-RNAINS4

   Plasmid

   #69456

   Purpose

   RNA-IN S4 variant 5' UTR sequence controlling expression of superfolder GFP (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)

   Depositor

   Julius Lucks

   Article

   Watters et al Nucleic Acids Res. 2015 Sep 8. pii: gkv8

   Insert

   RNA-IN S4 + SFGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   mutations for S4 variant (from wt)

   Promoter

   J23119 and J23119 (sigma 70)

   Available since

   Sept. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJBL-RNAINS3

   Plasmid

   #69455

   Purpose

   RNA-IN S3 variant 5' UTR sequence controlling expression of superfolder GFP (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)

   Depositor

   Julius Lucks

   Article

   Watters et al Nucleic Acids Res. 2015 Sep 8. pii: gkv8

   Insert

   RNA-IN S3
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   mutations for S3 variant (from wt)

   Promoter

   J23119 and J23119 (sigma 70)

   Available since

   Sept. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. LI D-E 11GFP

    Plasmid

    #54430

    Depositor

    Martin Parniske

    Article

    Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

    Insert

    FLAG tag + and short linker + superfolder GFP 11 domain
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. MDH1-pre-let-7_PShairpin

    Plasmid

    #26537

    Depositor

    Chang-Zheng Chen

    Article

    Trujillo et al EMBO J. 2010 Oct 6;29(19):3272-85. doi:

    Insert

    pre-let-7_PS hairpin
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    5 nt mutated to make pre-let-7 stem pairing perfe…

    Promoter

    Available since

    Jan. 11, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJK384.4

    Plasmid

    #155369

    Purpose

    dCas9 + PA4-mVenus

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas9 (bacteria)
    PA4-mVenus
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D10A H840A (catalytically inactive)

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-tetON-KRAB-dCas9-DHFR-EF1a-TagRFP-2A-tet3G

    Plasmid

    #167935

    Purpose

    Inducible CRISPRi expression

    Depositor

    Emma Rawlins

    Article

    Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

    Insert

    KRAB-dCas9-DHFR
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    tetON

    Available since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. SpyTag-sfGFP

    Plasmid

    #184226

    Purpose

    Expresses SpyTag-superfolder GFP in the bacterial cytoplasm. SpyTag enables covalent reaction with SpyCatcher.

    Depositor

    Mark Howarth

    Article

    Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:

    Insert

    SpyTag-sfGFP
    

    Use

    Tags

    His6, SpyTag, and TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLX_311-KRAB-dCas9

    Plasmid

    #96918

    Purpose

    for CRISPRi, lentiviral expression of KRAB-dCas9 and BlastR. Also called pXPR_121

    Depositor

    William Hahn, David Root, John Doench

    Article

    Rosenbluh et al Nat Commun. 2017 May 23;8:15403. doi: 10

    Insert

    dCas9
    

    Use

    Lentiviral

    Tags

    KRAB

    Expression

    Mammalian

    Mutation

    dCas9

    Promoter

    EF1a

    Available since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. LRG2.1

    Plasmid

    #108098

    Purpose

    Lentiviral expression plasmid of sgRNA with GFP

    Depositor

    Christopher Vakoc

    Article

    Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter for sgRNA expression and EFS promoter…

    Available since

    June 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. PiggyBac dCas9-KRAB

    Plasmid

    #191314

    Purpose

    piggyBac integration and dox-inducible expression of KRAB-dCas9-HA-tagBFP

    Depositor

    Joanna Wysocka

    Article

    Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

    Inserts

    KRAB-dCas9-HA-tagBFP
    rtTA3-IRES-PURO
    

    Use

    CRISPR

    Tags

    HA and tagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV hUbC-dCas9-T2A-GFP

    Plasmid

    #53191

    Purpose

    Co-expresses human optimized S. pyogenes dCas9 and GFP

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Insert

    humanized dead Cas9 T2A GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    D10A and H840A

    Promoter

    hUbC

    Available since

    June 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. 4xHRE_YB TATA-sfGFP-CMV_dsRed

    Plasmid

    #117399

    Purpose

    sfGFP expressed from hypoxia-inducible YB_TATA promoter and dsRed expressed from consitutive CMV promoter

    Depositor

    Yvonne Chen

    Article

    Ede et al ACS Synth Biol. 2016 May 20;5(5):395-404

    Insert

    SuperFolder GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pdCas9-bacteria

    Plasmid

    #44249

    Purpose

    aTc-inducible expression of a catalytically inactive bacterial Cas9 (S. pyogenes) for bacterial gene knockdown

    Depositor

    Stanley Qi

    Article

    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

    Insert

    dCas9 (bacteria)
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D10A H840A (catalytically inactive)

    Promoter

    pLtetO-1

    Available since

    April 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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