We narrowed to 11,272 results for: HAL

1. pcDNA5/FRT/TO-DDX54-FLAG/HA

   Plasmid

   #97062

   Purpose

   Expresses DDX54-FLAG/HA (C-terminal tagged) in mammalian cells

   Depositor

   Markus Landthaler

   Article

   Milek et al Genome Res. 2017 Aug;27(8):1344-1359. do

   Insert

   DDX54 (DDX54 Human)
   

   Use

   Tags

   FLAG/HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-mCherry-FKBP-GAP(ARHGAP29)

   Plasmid

   #222632

   Purpose

   Rho inactivation using rapamycin

   Depositor

   Arnold Hayer

   Article

   Marshall-Burghardt et al Sci Adv. 2024 Sep 6;10(36):eadn6858. doi

   Insert

   mCherry-FKBP-GAP(ARHGAP29) (ARHGAP29 Human)
   

   Use

   Tags

   mCherry-FKBP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. mCherry-CAD

   Plasmid

   #73566

   Purpose

   Soluble red fluorescent activator of Orai1 channels

   Depositor

   Michael Cahalan

   Article

   Dynes et al Proc Natl Acad Sci U S A. 2016 Jan 12;11

   Insert

   mCherry-CAD (STIM1 342-448) (STIM1 Synthetic, Human)
   

   Use

   Tags

   6x His and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV Immediate Early

   Available since

   March 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSAVED-CHAT_PCaspase_PCi_PC-σ

   Plasmid

   #214040

   Purpose

   Bacterial expression plasmid for SAVED-CHAT, PCaspase, PCi, and PC-σ from Haliangium ochraceum

   Depositor

   Raymond Staals

   Article

   Steens et al Science. 2024 Feb 2;383(6682):512-519. d

   Insert

   SAVED-CHAT-Pcaspase-Pci-PC-σ
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   lacUV5 promoter

   Available since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. CMV-CAD

   Plasmid

   #73567

   Purpose

   Soluble activator of Orai1 channels

   Depositor

   Michael Cahalan

   Article

   Dynes et al Proc Natl Acad Sci U S A. 2016 Jan 12;11

   Insert

   CAD (STIM1 342-448) (STIM1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV Immediate Early

   Available since

   March 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFRT/TO/HIS/FLAG/HA-EDF1

   Plasmid

   #38079

   Depositor

   Markus Landthaler

   Article

   Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

   Insert

   EDF1 endothelial differentiation-related factor 1 (EDF1 Human)
   

   Use

   Tags

   HIS/FLAG/HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFRT/TO/HIS/FLAG/HA-YTHDF2

   Plasmid

   #38089

   Depositor

   Markus Landthaler

   Article

   Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

   Insert

   YTHDF2 YTH domain family, member 2 (YTHDF2 Human)
   

   Use

   Tags

   HIS/FLAG/HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRS423-mitoT

   Plasmid

   #174525

   Purpose

   Expresses yeast variant of mitoT synthetic intermembrane tether bridging the inner and outer mitochondrial membranes

   Depositor

   Oleh Khalimonchuk

   Article

   Dietz et al Redox Biol. 2021 Oct;46:102125. doi: 10.

   Insert

   Yeast mitochondrial intermembrane tether
   

   Use

   Synthetic Biology

   Tags

   eGFP

   Expression

   Yeast

   Mutation

   Promoter

   MET25

   Available since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFRT/TO/HIS/FLAG/HA-CSNK1E

   Plasmid

   #38078

   Depositor

   Markus Landthaler

   Article

   Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

   Insert

   CSNK1E casein kinase 1, epsilon (CSNK1E Human)
   

   Use

   Tags

   HIS/FLAG/HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pETM33_Orf9b

    Plasmid

    #156485

    Purpose

    Bacterial expression of Sars-CoV2 Orf9b protein with His-tag and GST-tag

    Depositor

    Ylva Ivarsson

    Article

    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

    Insert

    Orf9b
    

    Use

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Gene insert is codon optimized for Ecoli.

    Promoter

    T7/LacO

    Available since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. LF701: pMAGIC (L1-R5) hU6::xCas9(3.7) gRNA scaffold

    Plasmid

    #121817

    Purpose

    pMAGIC L1-R5 entry plasmid, contains empty human U6-driven xCas9(3.7) (i.e. SpCas9) gRNA scaffold for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. KZ801: pMAGIC (L1-R5) hU6::SaCas9 gRNA scaffold; EF1a promoter

    Plasmid

    #121814

    Purpose

    pMAGIC L1-R5 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and EF1a promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. actc1b-mKate2-SERCA1

    Plasmid

    #109498

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to sarcoplasmic/endoplasmic calcium ATPase 1

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    SERCA1 (atp2a1 Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    mKate2

    Expression

    Mutation

    Please see Depositor Comments

    Promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNUT N6His K206E/K534A hTFNG

    Plasmid

    #70133

    Purpose

    Expresses N-His tagged nonglycosylated human serum transferrin unable to release iron from the N-lobe or the C-lobe

    Depositor

    Ross MacGillivray, Anne Mason

    Article

    Halbrooks et al Biochemistry. 2003 Apr 8;42(13):3701-7.

    Insert

    N6His K206e/K534A hTFNG (TF Human)
    

    Use

    Tags

    N-terminal signal peptide, 4 aa link, 6 His, Fact…

    Expression

    Mammalian

    Mutation

    changed Lys206 to a glutamic acid and Lys534 to a…

    Promoter

    SV40

    Available since

    Nov. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. G-GECO1.2-Orai1Y80E

    Plasmid

    #73565

    Purpose

    Green fluorescent reporter of mutant Orai1Y80E-associated calcium influx

    Depositor

    Michael Cahalan

    Article

    Dynes et al Proc Natl Acad Sci U S A. 2016 Jan 12;11

    Insert

    G-GECO1.2-Orai1Y80E (ORAI1 Synthetic, Human)
    

    Use

    Tags

    G-GECO1.2

    Expression

    Mammalian

    Mutation

    changed tyrosine 80 of Orai1 to glutamate

    Promoter

    CMV Immediate Early

    Available since

    March 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. JI501: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::SaCas9 gRNA scaffold

    Plasmid

    #121841

    Purpose

    pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; empty hU6-driven SaCas9 gRNA scaffold for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to dCas9 w/ gRNA expression

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFRT/TO/HIS/FLAG/HA-API5

    Plasmid

    #38074

    Depositor

    Markus Landthaler

    Article

    Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

    Insert

    API5 apoptosis inhibitor 5 (API5 Human)
    

    Use

    Tags

    HIS/FLAG/HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMS04 [myo-3p::bPGC::SL2::mCherry]

    Plasmid

    #168172

    Purpose

    Expression of bPGC in BWMs of C. elegans

    Depositor

    Alexander Gottschalk

    Article

    Woldemariam et al Genetics. 2019 Jul 22. pii: genetics.119

    Insert

    bPGC
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    May 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. LG002: pMAGIC (L1-R5) mU6::xCas9(3.7) gRNA scaffold

    Plasmid

    #121816

    Purpose

    pMAGIC L1-R5 entry plasmid, contains empty mouse U6-driven xCas9(3.7) (i.e. SpCas9) gRNA scaffold for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA5/BirA/FLAG-DDX54

    Plasmid

    #97063

    Purpose

    Expresses BirA/FLAG-tagged DDX54 in mammalian cells

    Depositor

    Markus Landthaler

    Article

    Milek et al Genome Res. 2017 Aug;27(8):1344-1359. do

    Insert

    DDX54 (DDX54 Human)
    

    Use

    Tags

    BirA and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    tetO2

    Available since

    July 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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