We narrowed to 12,145 results for: SHA
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Plasmid#74376PurposegRNA_A to knockout human AMPK alpha 2 using Cas9n.DepositorAvailable SinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only
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pLX304-YAP1 _60-89
Plasmid#59144PurposeYAP1 mutants that harbored a deletion of amino acide at 60-89 position, which disrupts binding to TEAD transcription factorsDepositorAvailable SinceAug. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLX304-YAP1 _PDZ
Plasmid#59147PurposeYAP1 mutants that harbored a deletion of the 5 amino acidsDepositorInsertYAP1 (YAP1 Human)
UseLentiviralExpressionMammalianMutationPDZ binding domain (last 5aa) deletedPromoterCMVAvailable SinceOct. 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR - EGFP sgRNA 3
Plasmid#51762PurposeExpresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.DepositorInsertsCas9
Puromycin resistance
EGFP sgRNA 3
UseCRISPR and LentiviralExpressionMammalianPromoterEFS and U6Available SinceMarch 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pX462-hPRKAA2-gRNA_B
Plasmid#74377PurposegRNA_B to knockout human AMPK alpha 2 using Cas9nDepositorAvailable SinceApril 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pU6-(BsaI)_CBh-Cas9-T2A-mCherry
Plasmid#135012PurposeExpression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 linked to mCherry via a T2A peptideDepositorInserthumanized S. pyogenes Cas9
Tags3x Flag, 3xFLAG (N terminal on insert), NLS, and …ExpressionMammalianPromoterCBhAvailable SinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti.PGK.blast-Flag-MFF-WT
Plasmid#74379PurposeLentiviral expression vector containing flag tagged WT MFF isoform 5DepositorAvailable SinceApril 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag2-Sox9 T236A
Plasmid#111455PurposeTransient expression of Flag-tagged human Sox9 with T236A mutationDepositorAvailable SinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
EF1a_MYC Thr58Ala_P2A_Hygro_Barcode
Plasmid#120513PurposeBarcoded lentiviral vector to express MYC Thr58Ala in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertMYC Thr58Ala (MYC Human)
UseLentiviralMutationPoint mutation changing Threonine to Alanine at a…PromoterEF1aAvailable SinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNK115
Plasmid#219745PurposeMoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi luciferase nnLuz_v4 codon-optimised for expression in Nicotiana benthamianaDepositorInsertmutant of fungal luciferase
UseLuciferase and Synthetic BiologyMutationI3S, N4T, F11L, I63T, T99P, T192S, A199PAvailable SinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNK116
Plasmid#219742PurposeMoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 codon-optimised for expression in Nicotiana benthamianaDepositorInsertmutant of fungal hispidin-3-hydroxylase
UseSynthetic BiologyMutationD37E, V181I, S323M, M385KAvailable SinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Puro-sgMARK1
Plasmid#138688PurposeExpresses a human MARK1-targeting sgRNA and Cas9DepositorAvailable SinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2-GJA1-20K-V5
Plasmid#49859PurposeEncodes C-terminal fragment of human connexin 43 initiating at M213, with M281 and M320 mutated to L, and a V5 C-terminal fusion tagDepositorInsertGJA1-20k (GJA1 Human)
TagsV5ExpressionMammalianMutationGJA1-20k fragment corresponds to residues 213-382…PromoterCMVAvailable SinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR - EGFP sgRNA 6
Plasmid#51765PurposeExpresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.DepositorInsertsCas9
Puromycin resistance
EGFP sgRNA 6
UseCRISPR and LentiviralExpressionMammalianPromoterEFS and U6Available SinceMarch 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag2-Sox9 T240A
Plasmid#111457PurposeTransient expression of Flag-tagged human Sox9 with T240A mutationDepositorAvailable SinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLX304-YAP1 _SH3bm
Plasmid#59141PurposeYAP1 mutants that harbored a deletion of SH3 binding motif, which disrupts interaction of YES1 with YAP1DepositorAvailable SinceOct. 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
EF1a_MYC deltaNLS_P2A_Hygro_Barcode
Plasmid#120506PurposeBarcoded lentiviral vector to express c-MYC deltaNLS in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertMYC H84NLS (MYC Human)
UseLentiviralMutationDeletion of nuclear localization signal sequencePromoterEF1aAvailable SinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
EF1a_MYC Ser62Ala_P2A_Hygro_Barcode
Plasmid#120514PurposeBarcoded lentiviral vector to express MYC Ser62Ala in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertMYC Ser62Ala (MYC Human)
UseLentiviralMutationPoint mutation changing Serine to Alanine at amin…PromoterEF1aAvailable SinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Blast-sgCRTC2-1
Plasmid#138700PurposeExpresses a human CRTC2-targeting sgRNA and Cas9DepositorAvailable SinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Blast-sgCRTC2-2
Plasmid#138701PurposeExpresses a human CRTC2-targeting sgRNA and Cas9DepositorAvailable SinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only