We narrowed to 5,724 results for: chia

1. pT22gcfUbm5En25EE4PCh(#552)

   Plasmid

   #184092

   Purpose

   cyclofen-inducible chicken EN2 (mut) activation in zebrafish permanent transgenic

   Depositor

   Sophie Vriz

   Article

   Amblard et al Commun Biol. 2020 Sep 29;3(1):536. doi:

   Insert

   5myc-chkEn2(5E)-ERT2-P2A-mCherry
   

   Use

   Zebrafish transgenesis (blue eyes)

   Tags

   5xMyc

   Expression

   Mutation

   chkEn2: S150E, S151E, S153E, S155E, S156E; ERT2: …

   Promoter

   Zf-Ubi

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT22Ubm4Eng2bKRPC(#493)

   Plasmid

   #184091

   Purpose

   cyclofen-inducible fish Eng2b (mut) activation in zebrafish permanent transgenic

   Depositor

   Sophie Vriz

   Article

   Amblard et al Commun Biol. 2020 Sep 29;3(1):536. doi:

   Insert

   4myc-ZfEng2b(WW>KK)-ERT2-P2A-mCherry
   

   Use

   Zebrafish transgenesis (blue eyes)

   Tags

   4xMyc

   Expression

   Mutation

   ZfEng2b: W141K, W144K; ERT2: G400V, M543A, L544A,…

   Promoter

   Zf-Ubi

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. Lenti_CRISPRi_sgAR10

   Plasmid

   #167003

   Purpose

   Lentiviral expression of sgRNA targeted to the androgen receptor (AR) promoter for CRISPRi knockdown

   Depositor

   Gian-Paolo Dotto

   Article

   Ma et al J Exp Med. 2021 Feb 1;218(2). pii: 21150

   Insert

   AR Promoter sgRNA 10 (AR Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   April 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET15-His-∆NGlpG(C104V/D243G/L244G/F245G/M247C/S248G/M249G/A250G)

   Plasmid

   #155134

   Purpose

   Expresses N-terminally His-tagged E. coli GlpG C104V/D243G/L244G/F245G/M247C/S248G/M249G/A250G variant (residues 87-276) from pET15b

   Depositor

   Sinisa Urban

   Article

   Cho et al Nat Struct Mol Biol. 2019 Oct;26(10):910

   Insert

   GlpG (glpG Escherichia coli)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   C104A/D243G/L244G/F245G/M247C/S248G/M249G/A250G, …

   Promoter

   T7

   Available since

   Sept. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. rat brain inositol 1,4,5-trisphosphate 3-kinase A (Clone C5, partial sequence from V66)

   Plasmid

   #122450

   Purpose

   Expression of inositol 1,4,5-trisphosphate 3-kinase A

   Depositor

   Christophe Erneux

   Article

   Takazawa et al Biochem J. 1990 Nov 15;272(1):107-12.

   Insert

   inositol 1,4,5-trisphosphate 3-kinase A (Itpka Rat)
   

   Use

   Tags

   beta-galactosidase

   Expression

   Bacterial

   Mutation

   coding sequence from V66 till stop codon

   Promoter

   Available since

   March 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. N295A (EcN), E166Q (EcN) MetNI LmH

   Plasmid

   #118269

   Purpose

   N295A and E166Q Mutation of MetN in Methionine ABC Transporter with 6x His Tag. Used to solve crystal structure

   Depositor

   Douglas Rees

   Article

   Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p

   Inserts

   MetI (metI Escherichia coli)
   MetN (E166Q ; N295A) (metN Escherichia coli)
   

   Use

   Tags

   6x His Tag

   Expression

   Bacterial

   Mutation

   Changed Glutamic Acid (Glu) 166 to Glutamine (Gln…

   Promoter

   T7 Promoter

   Available since

   Nov. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBEST-p70a-UTR3-Nterm 6xHis mRFP K15TAG-T500

   Plasmid

   #92319

   Purpose

   Expression of NTerm 6xHis tagged mRFP mutant K15TAG for ncAA incoporation using the p70a promoter and UTR3

   Depositor

   Lital Alfonta

   Article

   Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

   Insert

   Optimized NTerm 6xHis tagged monomeric RFP (mRFP)
   

   Use

   Synthetic Biology; Cell free protein synthesis (c…

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   p70a

   Available since

   July 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBEST-p70a-UTR1-Nterm 6xHis mRFP K15TAG-T500

   Plasmid

   #92318

   Purpose

   Expression of NTerm 6xHis tagged mRFP mutant K15TAG for ncAA incoporation using the p70a promoter and UTR1

   Depositor

   Lital Alfonta

   Article

   Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

   Insert

   Optimized NTerm 6xHis tagged monomeric RFP (mRFP)
   

   Use

   Synthetic Biology; Cell free protein synthesis (c…

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   p70a

   Available since

   July 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBEST-p70a-UTR1-NTerm 6xHis-zmADH V86TAG-T500

   Plasmid

   #92230

   Purpose

   Expression of zmADH mutant V86TAG for ncAA incoporation using the p70a promoter and UTR1

   Depositor

   Lital Alfonta

   Article

   Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

   Insert

   NTerm 6xHis Tagged zmADH
   

   Use

   Synthetic Biology; Cell free protein synthesis (c…

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Residue V86 mutated into TAG (Amber) stop codon f…

   Promoter

   p70a

   Available since

   June 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAc-GFP-dSUMOdeltaC

    Plasmid

    #85697

    Purpose

    expression of GFP-SUMO1 delta C in Drosophila cells

    Depositor

    Licio Collavin, Giannino Del Sal

    Article

    Mauri et al J Biol Chem. 2008 Jul 25. 283(30):20848-

    Insert

    SUMO (Sumo Fly)
    

    Use

    Tags

    GFP

    Expression

    Insect

    Mutation

    non-conjugatable SUMO, lacking the C-terminal gly…

    Promoter

    pAc5

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

11. pETM6-1D4-mCherry

    Plasmid

    #73423

    Purpose

    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 1D4.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Promoter 1D4 (orthogonal T7-lac variant)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P1D4 (orthogonal T7-lac variant)

    Available since

    March 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pETM6-4A6-mCherry

    Plasmid

    #73424

    Purpose

    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 4A6.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Promoter 4A6 (orthogonal T7-lac variant)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P4A6 (orthogonal T7-lac variant)

    Available since

    March 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pETM6-1E4-mCherry

    Plasmid

    #73426

    Purpose

    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 1E4.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Promoter 1E4 (orthogonal T7-lac variant)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P1E4 (orthogonal T7-lac variant)

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pETM6-1B6-mCherry

    Plasmid

    #73420

    Purpose

    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 1B6.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Promoter 1B6 (orthogonal T7-lac variant)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P1B6 (orthogonal T7-lac variant)

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pETM6-3H5-mCherry

    Plasmid

    #73419

    Purpose

    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 3H5.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Promoter 3H5 (orthogonal T7-lac variant)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P3H5 (orthogonal T7-lac variant)

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-flex-taCasp3-TEVp

    Plasmid

    #45580

    Depositor

    Nirao Shah, Jim Wells

    Article

    Yang et al Cell. 2013 May 9;153(4):896-909. doi: 10

    Has Service

    AAV1 and AAV5

    Inserts

    Caspase 3 (CASP3 Human, Synthetic)
    Tobacco Etch Virus protease
    

    Use

    AAV; Adeno associated viral vector

    Tags

    Expression

    Mutation

    Linker replaced with a TEV protease cleavage site

    Promoter

    EF1a

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pNK3071

    Plasmid

    #219755

    Purpose

    MoClo-compatible Level P vector for improved autonomous bioluminescence in plants encoding kanamycin resistance cassette, mcitHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH.

    Depositor

    Alexander Mishin

    Article

    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

    Insert

    mcitHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. 5xUAS:GAP-EGFP-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2

    Plasmid

    #158652

    Purpose

    making transgenics expressing a UAS driven eGFP and NTR 2.0

    Depositor

    Jeff Mumm

    Article

    Sharrock et al Nat Methods. 2022 Feb;19(2):205-215. doi

    Insert

    5xUAS:GAP-eGFP-P2A-NfsB_Vv F70A/F108Y;he:BFP
    

    Use

    Tol2-based transgenic expression in zebrafish/oth…

    Tags

    Expression

    Mutation

    F70A/F108Y

    Promoter

    Available since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pNK497

    Plasmid

    #219754

    Purpose

    MoClo-compatible Level P vector for improved autonomous bioluminescence in plants encoding kanamycin resistance cassette, nnHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH.

    Depositor

    Alexander Mishin

    Article

    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

    Insert

    nnHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV-TetO-hNGN2-Neo

    Plasmid

    #99378

    Purpose

    Expresses human NEUROGENIN2 (hNGN2) and neomycin resistance gene under control of TetON promoter. 3rd generation lentiviral backbone.

    Depositor

    Kristen Brennand

    Article

    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

    Insert

    hNGN2-P2A-Neo (NEUROG2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetO

    Available since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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