We narrowed to 7,670 results for: URE

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pAPInt-mNeonGreen-Hyg

Plasmid

#236483

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with mNeonGreen and selection on Hyg.

Depositor

Daniel Lew

Article

Colarusso et al Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi

Insert

mNeonGreen

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAPInt-mScarlet-Hyg

Plasmid

#236492

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with mScarlet and selection on Hyg.

Depositor

Daniel Lew

Article

Colarusso et al Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi

Insert

mScarlet

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAPInt-GFP-Hyg

Plasmid

#236477

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with GFP and selection on Hyg.

Depositor

Daniel Lew

Article

Colarusso et al Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi

Insert

GFP

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pBA1802

Plasmid

#246067

Purpose

Bacterial expression of mutant T4 Acb1 (H44A,H113A) under control of the pJex promoter

Depositor

Jennifer Doudna

Article

Doherty et al Nature. 2025 Nov;647(8091):997-1004. doi

Insert

Anti CBASS 1

Expression

Bacterial

Mutation

H44A,H113A

Promoter

pJEX promoter

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pSR1154

Plasmid

#221653

Purpose

Tetracycline sensor v3 for Staphylococcus aureus

Depositor

Lauren Andrews

Article

Rondthaler et al ACS Synth Biol. 2023 Dec 8. doi: 10.1021

Inserts

sfGFP cassette
TetR cassette

Use

Synthetic Biology

Expression

Bacterial

Promoter

PXyl-TetO and PZwf

Available Since

March 5, 2025

Availability

Academic Institutions and Nonprofits only

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pCN38-ktrA-yfp

Plasmid

#227636

Purpose

c-di-AMP biosensor plasmid. Encodes yfp downstream of c-di-AMP-binding ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and chloramphenicol resistance to S. aureus.

Depositor

Angelika Grundling

Article

Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

Insert

ktrA-yfp

Expression

Bacterial

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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UMG-SP-3_pET29b

Plasmid

#215435

Purpose

Expression construct for UMG-SP-3 gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

UMG-SP-3

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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UMG-SP-1_pET29b

Plasmid

#215433

Purpose

Expression construct for UMG-SP-1 gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

UMG-SP-1

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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UMG-SP-2_pET29b

Plasmid

#215434

Purpose

Expression construct for UMG-SP-2 gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

UMG-SP-2

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pEPSA5Cas9syn

Plasmid

#206965

Purpose

S. aureus genome editing vector removed these two motifs AGGNNNNNGAT and CCAYNNNNNNTGT

Depositor

Daijie Chen

Article

Yang et al ACS Synth Biol. 2023 Oct 13. doi: 10.102

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR1a-MTS-jAspSnFR3

Plasmid

#203461

Purpose

Mitochondrial directed jAspSnFR3 in Gateway cloning entry vector

Depositor

Lucas Sullivan

Article

Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

Insert

MTS-jAspSnFR3

Use

Gateway Cloning

Tags

Mitochondrial localization signal

Available Since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pTAG1

Plasmid

#176863

Purpose

First sgRNA in a tRNA separated array of sgRNAs

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

sgRNA_1

Use

CRISPR

Mutation

WT

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pTAG2E

Plasmid

#176865

Purpose

First sgRNA in a tRNA separated array of sgRNAs

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

sgRNA_2E

Use

CRISPR

Mutation

WT

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pET28_hTLN2-head

Plasmid

#164836

Purpose

Expression of human talin2 head

Depositor

Tina Izard

Article

Rangarajan et al J Biol Chem. 2020 Sep 11;295(37):12885-1

Insert

TLN2 (TLN2 Human)

Tags

His8

Expression

Bacterial

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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T5-sgRNA

Plasmid

#113655

Purpose

sgRNA targeting unit plasmid. The sgRNA targeting unit plasmids contain connector ConLS, ConR1, and one sgRNA transcriptional unit.

Depositor

Jeffrey Barrick

Article

Geng et al ACS Synth Biol. 2019 Mar 15;8(3):521-531

Insert

promoter T5 and sgRNA

Use

CRISPR

Expression

Bacterial

Promoter

T5

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pAW206

Plasmid

#113246

Purpose

E. coli expression vector for IHFa K15A + IHFb

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

IHFa K15A (ihfA E. coli)
IHFb

Expression

Bacterial

Mutation

K15A

Promoter

T7

Available Since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pKN3097

Plasmid

#113392

Purpose

Galactose-inducible Ty3 with extra PPT- used for random bar code library

Depositor

Suzanne Sandmeyer

Article

Patterson et al Genome Res. 2019 Aug;29(8):1298-1309. do

Insert

Ty3

Expression

Yeast

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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p3E NWASP-CA

Plasmid

#105968

Purpose

3' element for Gateway cloning, NWASP C terminal domain

Depositor

Caren Norden

Article

Icha et al J Cell Biol. 2016 Oct 24;215(2):259-275.

Insert

NWASP-CA

Expression

Bacterial

Available Since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pJK547

Plasmid

#72449

Purpose

Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide synthetase, Val319>Phe mutant (AaPurK-V319F)

Depositor

T. J. Kappock

Insert

N5-carboxyaminoimidazole ribonucleotide synthetase

Expression

Bacterial

Mutation

Val319 to Phe

Promoter

T7

Available Since

Feb. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pRL-TK let7 H

Plasmid

#11331

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

2 binding sites for let-7a miRNA

Use

Luciferase

Tags

Rr-luc

Mutation

Mutation H from figure 4A of paper.

Available Since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only