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pMKO.1 puro hTERT shRNA

Plasmid

#10688

Depositor

William Hahn

Article

Masutomi et al Proc Natl Acad Sci U S A. 2005 Jun 7. 10

Insert

hTERT shRNA (TERT Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Jan. 6, 2006

Availability

Academic Institutions and Nonprofits only

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PL-SIN-PGK-EiP

Plasmid

#21312

Purpose

Control Lentiviral vector with ubiquitous PGK promoter, expresses EGFP and Puro resistance

Depositor

James Ellis

Article

Hotta et al Nat Methods. 2009 May . 6(5):370-6.

Insert

Enhanced Green Fluorescence Protein, Puromycin resistance gene

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

June 1, 2009

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-RiboL1-jGCaMP8m

Plasmid

#192613

Purpose

AAV transfer plasmid for CAG mediated expression of soma-targeted (RiboL1) jGCaMP8m.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

RiboL1-jGCaMP8m

Use

AAV

Tags

6xHis

Expression

Mammalian

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-PCNA-IRES-puro2b

Plasmid

#26461

Depositor

Daniel Gerlich

Article

Held et al Nat Methods. 2010 Sep . 7(9):747-54.

Insert

PCNA (PCNA Human)

Tags

pEGFP

Expression

Bacterial and Mammalian

Available Since

Dec. 1, 2010

Availability

Academic Institutions and Nonprofits only

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YIP sHRPa-Aga1P

Plasmid

#73151

Purpose

Large fragment of split HRP fused to the N-terminus of the yeast mating protein Aga1P. When linearized, this vector incorporates into the yeast genome.

Depositor

Alice Ting

Article

Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

Insert

sHRPa-Aga1P

Tags

V5 tag

Expression

Yeast

Promoter

glyceraldehyde-3-phosphate dehydrogenase GPD prom…

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

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pHsp83-NLS-HA-2xMCP-2xTagRFP-T

Plasmid

#71242

Purpose

Used to label reporter mRNAs. Drosophila expression of the MCP protein fused to TagRFP-T.

Depositor

Anne Ephrussi

Article

Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

Insert

NLS-2xMCP-2xTagRFP-T

Use

P element-mediated germline transformation

Tags

TagRFP-T

Expression

Insect

Promoter

Hsp83

Available Since

Dec. 9, 2015

Availability

Academic Institutions and Nonprofits only

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JT113-pETDuet1-(R)-hREV3L

Plasmid

#64872

Purpose

bacterial expression of human REV3L

Depositor

Richard Wood

Article

Tomida et al Nucleic Acids Res. 2015 Jan;43(2):1000-1

Insert

REV3L (REV3L Human)

Expression

Bacterial

Promoter

T7

Available Since

July 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCS-H2B-mRFP1

Plasmid

#53745

Purpose

Contains a fusion of human histone H2B to monomeric red fluorescent protein 1 in the pCS2+ expression vector

Depositor

Sean Megason

Article

Megason et al Methods Mol Biol. 2009;546:317-32. doi:

Insert

histone H2B (H2BC21 Human)

Tags

mRFP

Expression

Mammalian

Promoter

simian CMV IE94

Available Since

July 8, 2014

Availability

Academic Institutions and Nonprofits only

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pRK5_mCherry-GFP-nb

Plasmid

#238259

Purpose

For overexpression of mCherry-GFP-nb

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

mCherry-GFP-nb

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_ALFA-nb

Plasmid

#238262

Purpose

For overexpression of ALFA-nb

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

ALFA-nb

Expression

Mammalian

Available Since

June 4, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_VHH05-nb-KS

Plasmid

#238270

Purpose

For overexpression of VHH05-nb-KS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

VHH05-nb-KS

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_ALFA-nb-KS

Plasmid

#238263

Purpose

For overexpression of ALFA-nb-KS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

ALFA-nb-KS

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_VHH05-nb-KS-FtoG

Plasmid

#238271

Purpose

For overexpression of VHH05-nb-KS-FtoG

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

VHH05-nb-KS-FtoG

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_VHH05-nb-noKS

Plasmid

#238269

Purpose

For overexpression of VHH05-nb-noKS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

VHH05-nb-noKS

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dPguCas13b-3xEGFP-NLS-3xFlag

Plasmid

#132399

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPguCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

H151A; H1121A

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pMH-SFB-RNF8

Plasmid

#99396

Purpose

Mammalian expression construct for S protein-Flag-Streptavidin binding peptide (SFB)-tagged RNF8

Depositor

Junjie Chen, Michael Huen

Article

Huen et al Cell. 2007 Nov 30;131(5):901-14. Epub 20

Insert

RNF8 (RNF8 Human)

Tags

SFB (S protein-FLAG-Streptavidin binding peptide)

Expression

Mammalian

Available Since

Aug. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pICE-EGFP-FLAG-Ku70siR-WT

Plasmid

#46961

Purpose

Plasmid for constitutive or doxycycline-inducible expression of wild-type human Ku70 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

Depositor

Steve Jackson

Article

Britton et al J Cell Biol. 2013 Jul 29.

Insert

human Ku70 (XRCC6 Human)

Tags

GFP-FLAG

Expression

Mammalian

Promoter

CMV Tet

Available Since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only

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Stx4-pmCitrine

Plasmid

#92422

Purpose

Expression of syntaxin-4 C-terminally conjugated to mCitrine.

Depositor

Geert van den Bogaart

Article

Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

Insert

Stx4 (Stx4 Rat)

Tags

mCitrine

Expression

Mammalian

Promoter

CMV

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-mClover3-anti-rabbit-IgG(Fc)-nanobody

Plasmid

#138129

Purpose

Expresses GST-tagged mClover3-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.

Depositor

Kazuhisa Nakayama

Article

Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

Insert

mClover3-anti-rabbit-IgG(Fc)-nanobody

Tags

GST

Expression

Bacterial

Available Since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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GW1-mito-mCherryEA

Plasmid

#207868

Purpose

Mitochondria-targeted mCherry(I158E/Q160A) mutant as a red fluorescent pH sensor

Depositor

Mathew Tantama

Article

Rajendran et al ACS Omega. 2018 Aug 31;3(8):9476-9486. d

Insert

mito-mCherryEA

Tags

4xCox8 mitochondrial targeting

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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