We narrowed to 19,645 results for: tec

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pRS423-CUP1-His-Ubi

Plasmid

#99541

Purpose

Yeast episomal vector for expression of His-tagged ubiquitin; HIS3 marker

Depositor

Helle Ulrich

Article

Davies et al Methods Mol Biol. 2012;920:543-67. doi:

Insert

UBI3(1-76) (RPL40A Budding Yeast)

Tags

7xHis

Expression

Yeast

Promoter

CUP1

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pX330A_dCas9-1x7

Plasmid

#63601

Purpose

Expresses dCas9 and gRNA

Depositor

Takashi Yamamoto

Article

Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

Insert

humanized S. pyogenes dCas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

June 1, 2015

Availability

Academic Institutions and Nonprofits only

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pJ211_rha_WT_low

Plasmid

#128851

Purpose

Low copy number plasmid for expression of E. coli flagellin from a rhamnose-inducible promoter

Depositor

Mark Pallen

Article

Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

Insert

Flagellin

Expression

Bacterial

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pTlpA_mCherry

Plasmid

#229146

Purpose

Promoter strength evaluation of PtlpA

Depositor

Shrikrishnan Sankaran

Article

Dey et al Microb Biotechnol. 2023 Jun;16(6):1264-1

Insert

mCherry

Expression

Bacterial

Mutation

No

Promoter

PtlpA

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCHARGE3

Plasmid

#24658

Depositor

Tanya Parish

Article

Carroll et al PLoS One. 2010 . 5(3):e9823.

Insert

Turbo

Expression

Bacterial

Available Since

June 9, 2010

Availability

Academic Institutions and Nonprofits only

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pMT445

Plasmid

#139386

Purpose

aTC inducible Nanoluc expression, pNBU1 backbone with BsaI sites, AmpR, ErmR

Depositor

Christopher Voigt

Article

Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.

Insert

aTC inducible Nanoluc expression

Use

Synthetic Biology

Promoter

aTC inducible promoter

Available Since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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pYL322d1/N-eGFP

Plasmid

#183151

Purpose

Plant transient expression vector containing eGFP tag. Unique nucleotide sequences Uc and Ud for cloning at N terminus of eGFP.

Depositor

Yao-Guang Liu, Qinlong Zhu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Type

Empty backbone

Expression

Plant

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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p8.9 NdeltaSB D185K/D186L

Plasmid

#132931

Purpose

p8.9 NdeltaSB bearing RT-D185K/D186L mutant reverse transcriptase sequence

Depositor

Jeremy Luban

Article

De Iaco et al Retrovirology. 2011 Dec 6;8:98. doi: 10.

Insert

gag and pol D185K/D186L

Use

Lentiviral

Mutation

D185K/D186L

Available Since

Oct. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pRC319

Plasmid

#61272

Purpose

Also referred to as pΦRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA targeting the NDM-1 gene. Contains a ColE1 origin, kanamycin resistance cassette and f1 origin for packaging in M13 particles.

Depositor

Timothy Lu

Article

Citorik et al Nat Biotechnol. 2014 Sep 21. doi: 10.103

Insert

Cas9, tracrRNA, crRNA

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Available Since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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pIVT-Cas9-DEN2

Plasmid

#238017

Purpose

for DNA-free gene knockout editing in rice and wheat or other plants

Depositor

Caixia Gao

Article

Qiu et al Plant Biotechnol J. 2025 Apr;23(4):1348-

Insert

SpCas9

Expression

Plant

Promoter

T7

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pAG1238 PINK1-mCherry-FIREtag

Plasmid

#205174

Purpose

Expresses PINK1-mCherry-FIREtag in mammalian cells

Depositor

Arnaud Gautier

Article

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Insert

PINK1-mCherry-FIREtag

Expression

Mammalian

Available Since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v2 sgATG7

Plasmid

#211534

Purpose

Deletes ATG7

Depositor

Richard Possemato

Article

Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

Insert

sgATG7

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pMT444

Plasmid

#139385

Purpose

IPTG inducible Nanoluc expression, pNBU1 backbone with BsaI sites, AmpR, ErmR

Depositor

Christopher Voigt

Article

Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.

Insert

IPTG inducible Nanoluc expression,

Use

Synthetic Biology

Promoter

IPTG inducible promoter

Available Since

July 15, 2023

Availability

Academic Institutions and Nonprofits only

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mSA-phi29

Plasmid

#210815

Purpose

in vitro transcription of mRNA

Depositor

Erik Sontheimer

Article

Liu et al Nat Biotechnol. 2023 Sep 14. doi: 10.103

Insert

mSA-Phi29

Expression

Bacterial

Available Since

Feb. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pUC-DEST-hPROX1-tdTomato-PuroTK

Plasmid

#141027

Purpose

Reporter integration

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

tdTomato

Expression

Mammalian

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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dTomato_LD 0_TEV

Plasmid

#58872

Purpose

MESA protease chain with dTomato ectodomain and no linker domain

Depositor

Joshua Leonard

Article

Daringer et al ACS Synth Biol. 2014 Mar 11.

Insert

MESA protease chain with dTomato ectodomain and TEV protease

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pFPL-Ch

Plasmid

#61647

Purpose

Gateway vector for generating cyan fluorescent protein fusions in fungi

Depositor

Mark Farman

Article

Gong et al Mol Plant Microbe Interact. 2014 Nov 12.

Insert

enhanced cyan fluorescent protein

Expression

Plant

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pFastBac StrepII msfGFP TEV cloning vector with BioBrick polycistronic restriction sites(11R-GFP)

Plasmid

#48297

Depositor

Scott Gradia

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Tags

StrepII and msfGFP

Expression

Insect

Available Since

Sept. 23, 2013

Availability

Academic Institutions and Nonprofits only

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pET28c-Ku

Plasmid

#161936

Purpose

Expresses 6xHis-tagged Ku from Mycobacterium tuberculosis under T7 promoter for purification

Depositor

Harris Wang

Article

Yim et al ACS Synth Biol. 2020 Sep 29. doi: 10.102

Insert

ku (mku Mycobacterium tuberculosis)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pSQT817

Plasmid

#53373

Purpose

wildtype Cas9 expression plasmid

Depositor

Keith Joung

Article

Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

Insert

Cas9

Use

CRISPR

Expression

Mammalian

Promoter

CAG

Available Since

July 8, 2014

Availability

Academic Institutions and Nonprofits only

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