Purpose
Expresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V1 (R887E) and hPGK promoter driven mCherry
Inserts
S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V1 (R887E) engineered fusion
mCherry
Mutation
D10A and H840A in S.pyogenes Cas9; R887E in Dnmt3A
Available Since
Feb. 7, 2025
Availability
Academic Institutions and Nonprofits only