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pSP64R1 SHP2 D61A

Plasmid

#8326

Depositor

Ben Neel

Article

O'Reilly et al Mol Cell Biol 2000 Jan;20(1):299-311.

Insert

SHP2 (PTPN11 Human)

Use

Xenopus expression

Mutation

D61A (activated mutant)

Available Since

June 17, 2005

Availability

Academic Institutions and Nonprofits only
rag2:mcherry_CE_pISceI

Plasmid

#223047

Purpose

Express m-Cherry gene in mesenchymal lineage of Zebrafish.

Depositor

Alejandro Gutierrez

Article

Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

Insert

m-Cherry

Use

Express the insert(gene) in mesenchymal lineage o…

Promoter

Rag2

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pMVS1111A:PhmtB-bgaB

Plasmid

#205998

Purpose

Conjugative shuttle vector for Escherichia coli and Methanothermobacter thermautotrophicus encoding beta-galactosidase

Depositor

Bastian Molitor

Article

Fink et al mBio. 2021 Dec 21;12(6):e0276621. doi: 1

Insert

thermostable beta-galactosidase

Promoter

PhmtB

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pSL1045

Plasmid

#129525

Purpose

pDEF-SpCas9, Empty Vector for CRISPRi, PbEF1a promoter, 1st Generation

Depositor

Scott Lindner

Article

Walker et al J Biol Chem. 2019 Jun 14;294(24):9555-95

Insert

dSpCas9 (Other)

Use

Plasmodium, rodent-infectious species

Available Since

Sept. 10, 2019

Availability

Academic Institutions and Nonprofits only

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T7-AglC-His6

Plasmid

#89726

Purpose

Expresses AglC from M. voltae with an N-terminal T7 tag and a C-terminal His6 tag. Plasmid has codons optimized for E. coli expression.

Depositor

Barbara Imperiali

Article

Zamora et al Methods Enzymol. 2017;597:145-186. doi:

Insert

AglC

Tags

His6 and T7

Expression

Bacterial

Available Since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pJH2523

Plasmid

#191358

Purpose

Punc-25-GCaMP3::UrSL2 ::wCherry unc-54 3' UTR C.elegans DD neurou expression of GCaMP3 RFP

Depositor

Mei Zhen

Article

Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

Insert

GCaMP3

Tags

mCherry

Expression

Worm

Promoter

Punc-25

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pJH3628

Plasmid

#191445

Purpose

Prgef-1-Chrimson::UrSL2::wCherry unc-54 3' UTR C.elegans panneural expression of Chrimson RFP

Depositor

Mei Zhen

Article

Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

Insert

Chrimson

Tags

wCherry

Expression

Worm

Promoter

Pregf-1

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pJH4329

Plasmid

#191679

Purpose

Prgef-1-GtACR2::wCherry unc-54 3' UTR C.elegans Pan-neural expression of GtACR2 RFP

Depositor

Mei Zhen

Article

Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

Insert

GtACR2

Tags

wCherry

Expression

Worm

Promoter

Pregf-1

Available Since

Nov. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pJH4330

Plasmid

#191700

Purpose

Punc-17::GtACR2::wCherry unc-54 3' UTR C.elegans neuron expression of GtACR2 RFP

Depositor

Mei Zhen

Article

Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

Insert

GtACR2

Tags

wCherry

Expression

Worm

Promoter

Punc-17

Available Since

Nov. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-20nmEML-mRFP

Plasmid

#231178

Purpose

Stabilizes the ER-mitochondrial distance at 20 nm; tagged with mRFP

Depositor

Dmitry Lim

Article

Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

Insert

20nmEML-mRFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-30nmEML-mRFP

Plasmid

#231179

Purpose

Stabilizes the ER-mitochondrial distance at 30 nm; tagged with mRFP

Depositor

Dmitry Lim

Article

Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

Insert

30nmEML-mRFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-H3.3(WT)-3AID-HA-2A-mCherryBSD

Plasmid

#225697

Purpose

Lentiviral expression of AID degron tagged Wildtype H3.3 and mCherry fused BlasticidinR

Depositor

Lacra Bintu

Article

Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

Insert

H3.3 (WT) (H3-3A, H3-3B Human)

Use

Lentiviral and Synthetic Biology

Tags

3xAID HA and T2A-mCherry

Expression

Mammalian

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pAAVS1-Puro-TNNT2-Neo

Plasmid

#214013

Purpose

Expression of cardiac troponin T

Depositor

Paul Burridge

Article

Fetterman et al Cell Rep. 2024 May 28;43(5):114160. doi:

Insert

TNNT2 (TNNT2 Human)

Expression

Mammalian

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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MSCV-ASNS-OE-IRES-Thy1.1

Plasmid

#192947

Purpose

Asparagine synthetase overexpression in murine cells

Depositor

Ping-Chih Ho

Article

Fernandez-Garcia et al Cell Rep. 2022 Nov 15;41(7):111639. doi:

Insert

Asparagine synthetase (Asns Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

5'LTR

Available Since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pRG/III-hs-MBP-T43NTR-TrxA-H10

Plasmid

#121677

Purpose

Bacterial expression of neurotensin receptor fusion protein

Depositor

Reinhard Grisshammer, Richard Henderson

Article

Grisshammer et al Protein Expr Purif. 1997 Oct;11(1):53-60

Insert

neurotensin receptor (Ntsr1 Rat)

Tags

TrxA-H10

Expression

Bacterial

Mutation

deleted amino acids 1-42

Promoter

lac

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only
Ad-EFNA1

Plasmid

#102724

Purpose

Expression of EphrinA1

Depositor

Jin Chen

Article

Brantley-Sieders et al Cancer Res. 2006 Nov 1;66(21):10315-24.

Insert

EFNA1 (EFNA1 Human)

Use

Adenoviral

Available Since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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pRSET a sfMatryoshCaMP6s-T78H

Plasmid

#100021

Purpose

Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference LSSmOrange nested within the reporting superfolder cpGFP-T78H

Depositor

Wolf Frommer

Article

Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

Insert

sfMatryoshCaMP6s

Tags

6x HIS tag and Xpress_EK tag

Expression

Bacterial

Mutation

cpEGFP replaced with superfolder cpGFP and T78H m…

Promoter

T7

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pLgw EcoDam-V5-SRF

Plasmid

#98602

Purpose

Mammalian DamID lentiviral vector forSRF with Dam-V5 using Gateway cloning

Depositor

Richard Harvey

Article

Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

Insert

SRF (Srf Mouse)

Use

Lentiviral; Damid

Tags

Dam (DNA adenine methyltransferase) and V5

Expression

Mammalian

Promoter

Heat Shock Minimal Promoter

Available Since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

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MAGE-A12Pro pGL4basic

Plasmid

#78139

Purpose

luciferase reporter plasmid

Depositor

Heidi Schwarzenbach

Article

Wischnewski et al Mol Cancer Res. 2007 Jul;5(7):749-59.

Insert

MAGE-A12 (MAGEA12 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

contains -425/+115 of promoter

Promoter

MAGE-A12

Available Since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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pRSET FLII32Pglu-2.2m

Plasmid

#13566

Depositor

Wolf Frommer

Article

Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

Insert

FLIPglu F16A P32S-ECFP-D33R

Tags

6xHis and Citrine

Expression

Bacterial

Mutation

F16A, ECFP insert in binding protein

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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