We narrowed to 11,279 results for: Vars

1. pEGFP-N1-MITF-M

   Plasmid

   #38131

   Depositor

   Shawn Ferguson

   Article

   Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

   Insert

   MITF-M (MITF Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   deleted stop codon

   Promoter

   CMV

   Available since

   July 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFP-N1-MITF-A

   Plasmid

   #38132

   Depositor

   Shawn Ferguson

   Article

   Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

   Insert

   MITF-A (MITF Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   deleted stop codon

   Promoter

   CMV

   Available since

   July 31, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-T7-ABE8e-nSpCas9-NG-P2A-EGFP (KAC999)

   Plasmid

   #185914

   Purpose

   pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Insert

   human codon optimized ABE8e-nSpCas9-NG-P2A-EGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9-NG=D10A/L1111R/D1135V/G1218R/E1219F/A1322…

   Promoter

   CMV and T7

   Available since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. mAIRN HO

   Plasmid

   #207411

   Purpose

   Mammalian-cell-based plasmid system that expresses mAIRN (an R-loop-forming transcript) upon dox-induction, which will further cause head-ON transcription-replication conflict (HO TRC).

   Depositor

   Karlene Cimprich

   Article

   Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do

   Insert

   mAIRN
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. mAIRN CD

   Plasmid

   #207412

   Purpose

   Mammalian-cell-based plasmid system that expresses mAIRN (an R-loop-forming transcript) upon dox-induction, which will further cause co-directional transcription-replication conflict (CD TRC).

   Depositor

   Karlene Cimprich

   Article

   Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do

   Insert

   mAIRN
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-TadA8e-PcrA

   Plasmid

   #221228

   Purpose

   Express HACE Editor as a fusion of TadA-8e adenosine deaminase and PcrA helicase.

   Depositor

   Fei Chen

   Article

   Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

   Insert

   TadA8e-PcrA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMDJ20 - pEXP [Psmu-1 | Cre Recombinase (PATCs) | smu-1 UTR]

   Plasmid

   #159808

   Purpose

   Optimized enzyme for genome editing in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   pEXP [Psmu-1 | Cre Recombinase (PATCs) | smu-1 UTR]
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDAS12489_PEAR-GFP_2in1_2.0

   Plasmid

   #177186

   Purpose

   PEAR-GFP3 plasmid with a pegRNA that targets its own plasmid

   Depositor

   Ervin Welker

   Article

   Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

   Inserts

   EGFP split with an intron between amino acids 95-96
   pegRNA targeting its own plasmid
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   disrupted 5' splice site

   Promoter

   CMV and U6

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. CROPseq-tRFP

   Plasmid

   #230938

   Purpose

   Modified version of the original CROP-seq vector (Addgene #86708), expressing tRFP instead of puroR for FACS

   Depositor

   Hyejung Won

   Article

   Lee et al Cell. 2025 Jan 22:S0092-8674(24)01435-1.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   TagRFP

   Expression

   Mutation

   Promoter

   Available since

   Feb. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. Klotho (membrane)

    Plasmid

    #17712

    Depositor

    Hal Dietz

    Article

    Arking et al Proc Natl Acad Sci U S A. 2002 Jan 22. 9

    Insert

    Klotho (KL Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    P101S

    Promoter

    Available since

    April 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. mCerulean-Keratin-17

    Plasmid

    #55379

    Purpose

    Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 433, Emission: 475

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    Keratin (KRT18 Human)
    

    Use

    Tags

    mCerulean

    Expression

    Mammalian

    Mutation

    V282L

    Promoter

    CMV

    Available since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pEGFP-N1-MITF-D

    Plasmid

    #38133

    Depositor

    Shawn Ferguson

    Article

    Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

    Insert

    MITF-D (MITF Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    deleted stop codon

    Promoter

    CMV

    Available since

    Aug. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. 3xFLAG-SENP7

    Plasmid

    #42886

    Depositor

    Edward Yeh

    Article

    Bawa-Khalfe et al Proc Natl Acad Sci U S A. 2012 Oct 23;10

    Insert

    SENP7 (SENP7 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    T102A, R969C, L996S

    Promoter

    Available since

    April 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pENTR-HLA-A2_GFP

    Plasmid

    #85161

    Purpose

    HLA.A2_GFP fusion with 2 glycines in between

    Depositor

    Sébastien Walchli, Tone Fredsvik Gregers

    Article

    Walchli et al Eur J Immunol. 2014 Mar;44(3):774-84. do

    Insert

    HLA.A2 (HLA-A Human)
    

    Use

    Gateway entry vector

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV-LK03

    Plasmid

    #206512

    Purpose

    AAV packaging vector containing AAV2 rep and LK03 cap and partial AAV2 p5 promoter

    Depositor

    Mark Kay

    Article

    Lisowski et al Nature. 2014 Feb 20;506(7488):382-6. doi

    Insert

    LK03 capsid
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    p5

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDV24_AAV2_DART-VADAR_iRFP720-sensor

    Plasmid

    #200411

    Purpose

    AAV vector encoding a DART-VADAR sensor for iRFP720 mRNA

    Depositor

    James Collins

    Article

    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

    Insert

    DART VADAR sensor
    

    Use

    AAV and Synthetic Biology

    Tags

    MCP-ADAR2dd(E488Q)-NES, TagBFP, and mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUCC001

    Plasmid

    #124451

    Purpose

    Multi-species CRISPR/Cas9 coexpression system, optimized for Golden Gate cloning of gRNA targets

    Depositor

    John Morrissey

    Article

    Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

    Insert

    HH-BsaI
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    gRNA expression cassette from pUDP002 modified to…

    Promoter

    TDH3 promoter from S.cerevisiae (already present …

    Available since

    Sept. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. shh10

    Plasmid

    #64867

    Purpose

    This plasmid can be used in the triple transfection method of AAV vector production. It supplies the replication proteins from AAV2 and the engineered shh10 capsid protein.

    Depositor

    John Flannery, David Schaffer

    Article

    Klimczak et al PLoS One. 2009 Oct 14;4(10):e7467. doi:

    Insert

    shh10 cap
    

    Use

    AAV

    Tags

    Expression

    Mutation

    I319V, N451D, D532N

    Promoter

    Available since

    Aug. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLKO.1 puro_shTBK1_3185

    Plasmid

    #167294

    Purpose

    Lentiviral plKO.1 construct coding for a short-hairpin RNA targeting the human gene TBK1. Contains a puro resistance cassette.

    Depositor

    Agnel Sfeir

    Article

    Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

    Insert

    shRNA TBK1 (TBK1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEY41

    Plasmid

    #191045

    Purpose

    dat-1 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

    Depositor

    Eviatar Yemini, Oliver Hobert

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    dat-1 (dat-1 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    None

    Promoter

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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