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We narrowed to 15,966 results for: LEA

Showing: 8651 - 8700 of 15966 results

  1. pOpen-BstpolLF

    Plasmid
    #165553
    Purpose
    Fragment retains 5'-3' polymerase activity from full length Bst DNA Polymerase, while lacking 5'-3' exonuclease activity. Suitable for applications requiring thermophilic strand displacement.
    Depositor
    FreeGenes Project, Jennifer Molloy, Drew Endy
    Insert
    Bst DNA Polymerase, Large Fragment
    Use
    Synthetic Biology
    Available Since
    Aug. 16, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165553-plasmid-map-sequence-id-339898

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  3. mEos3.2-Nup50-N-10

    Plasmid
    #57479
    Purpose
    Localization: Nuclear Pore, Excitation: 507 / 572, Emission: 516 / 580
    Depositor
    Michael Davidson
    Insert
    Nup50 (NUP50 Human)
    Tags
    mEos3.2
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 5, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  4. C39m

    Plasmid
    #120972
    Purpose
    MoClo golden gate assembly CD part for ccdB (DNA gyrase toxin, modified synonymously to remove BsaI cut site; ccdA/C79m is antitoxin). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    ccdB toxin
    Use
    Synthetic Biology
    Mutation
    BsaI site removed
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120972-plasmid-map-sequence-id-236024

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  5. pTH385-GST-TEV-SUP35

    Plasmid
    #29744
    Depositor
    Tobias von der Haar
    Insert
    SUP35 gene encoding translation release factor 3 from S. cerevisiae (SUP35 Budding Yeast)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    wild type
    Available Since
    Aug. 8, 2011
    Availability
    Academic Institutions and Nonprofits only
    29744-plasmid-map-sequence-id-13828

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  6. pExp-PpiB

    Plasmid
    #112572
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and PpiB (peptidyl-prolyl cis-trans isomerase B from E. coli) fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8xHis-PpiB and Strep tag
    Expression
    Bacterial
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112572-plasmid-map-sequence-id-217222

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  7. BII-gR-PnTW

    Plasmid
    #133356
    Purpose
    Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications) coexpressed with tdTomato-NLS
    Depositor
    Blair Madison
    Insert
    tdTomato-NLS
    Tags
    HA-tagged tdTomato
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    CMV enhancer and hEf1a (CpG free)
    Available Since
    Jan. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    133356-plasmid-map-sequence-id-267393

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  9. pTH278-GST-TEV-SUP45

    Plasmid
    #29743
    Depositor
    Tobias von der Haar
    Insert
    SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    wild type
    Available Since
    Sept. 6, 2011
    Availability
    Academic Institutions and Nonprofits only
    29743-plasmid-map-sequence-id-13827

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  10. pcDNA3.1-hRIPK1-Flag-S320A

    Plasmid
    #112491
    Purpose
    Mammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.
    Depositor
    Junying Yuan
    Insert
    RIPK1 (RIPK1 Human)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    S320A
    Available Since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112491-plasmid-map-sequence-id-214534

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  12. pSBbi-Pur-AktAR2

    Plasmid
    #125195
    Purpose
    SB-transposon plasmid for stable expression of AktAR2 variant of FRET-based AKT activity reporter
    Depositor
    Eric Davis
    Insert
    AktAR2
    Use
    Transposon
    Tags
    6xHis, T7, and Xpress, mCerulean3 and cpVenus[E17…
    Expression
    Mammalian
    Promoter
    EF1a/RPBSA
    Available Since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    125195-plasmid-map-sequence-id-243600

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  14. pBac[3xP3-gTc'v;Tc'rps3P-Tc'H2Av_EGFP]

    Plasmid
    #86454
    Purpose
    Nuclear reporter (histone-tagged EGFP) construct with the Tribolium specific ribosomal protein subunit 3 promoter (driving ubiquitous expression); (Transformation plasmid; Black eye marker)
    Depositor
    Gregor Bucher
    Insert
    3xP3-gTc'v;Tc'rps3P-Tc'H2Av_EGFP
    Expression
    Insect
    Available Since
    March 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    86454-plasmid-map-sequence-id-170900

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  15. PP-HSP90 (3PEH)

    Plasmid
    #28267
    Depositor
    Cheryl Arrowsmith
    Insert
    PP-HSP90 (PFL1070c Plasmodium Falciparum)
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    contains N-terminal domain.
    Available Since
    April 14, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  16. 4YY8

    Plasmid
    #69961
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PF13_0238:MAC052-B07:C233961
    Use
    Baculovirus expression
    Tags
    His and TEV cleavage site
    Promoter
    polyhedrin
    Available Since
    Oct. 7, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  17. C67m

    Plasmid
    #120981
    Purpose
    MoClo golden gate assembly CD part for Bxb1-mScarleti-LAA (Bxb1 integrase tagged with an mScarlet-I reporter and an LAA degradation tag). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    Bxb1 integrase - mScarlet-i - LAA tag
    Use
    Synthetic Biology
    Tags
    ssrA degradation tag
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120981-plasmid-map-sequence-id-236036

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  18. pcDNA3.1-hRIPK1-Flag-K377R

    Plasmid
    #112489
    Purpose
    Mammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.
    Depositor
    Junying Yuan
    Insert
    RIPK1 (RIPK1 Human)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    K377R
    Available Since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112489-plasmid-map-sequence-id-214536

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  19. pAG472 H2B-iFAST

    Plasmid
    #130818
    Purpose
    Expresses H2B-iFAST in mammalian cells (nuclear labeling)
    Depositor
    Arnaud Gautier
    Insert
    H2B-iFAST
    Tags
    myc-tag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    130818-plasmid-map-sequence-id-254956

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  20. pSBbi-Puro_LPAR_AKAR-WT

    Plasmid
    #125206
    Purpose
    SB-transposon plasmid for stable expression of lipid raft-targeted FRET-based PKA activity reporter
    Depositor
    Eric Davis
    Insert
    LPAR_AKAR-WT
    Use
    Transposon
    Tags
    EV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…
    Expression
    Mammalian
    Promoter
    EF1a/RPBSA
    Available Since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    125206-plasmid-map-sequence-id-243604

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  21. 5C8G

    Plasmid
    #69968
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    Tb427tmp.01.1830:MAC054-B05:C234722
    Tags
    His and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7-lacO
    Available Since
    Oct. 7, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  25. PLXNA2

    Plasmid
    #28279
    Depositor
    Cheryl Arrowsmith
    Insert
    PLXNA2 (PLXNA2 Human)
    Tags
    His with TEV cleavage
    Expression
    Bacterial
    Mutation
    residues 1490-1600.
    Available Since
    Feb. 10, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  26. pcDNA3.1-hRIPK1-Flag-K599R

    Plasmid
    #112490
    Purpose
    Mammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.
    Depositor
    Junying Yuan
    Insert
    RIPK1 (RIPK1 Human)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    K599R
    Available Since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112490-plasmid-map-sequence-id-214537

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  27. pcDNA3.1-hRIPK1-Flag-K115R

    Plasmid
    #112488
    Purpose
    Mammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.
    Depositor
    Junying Yuan
    Insert
    RIPK1 (RIPK1 Human)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    K115R
    Available Since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112488-plasmid-map-sequence-id-214538

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  28. Top7 K39E K40E V48V K55E

    Plasmid
    #12464
    Purpose
    Plasmid contains Top7 K39E K40E V46A and K55E. Please note this is DIFFERENT than what the plasmid name implies.
    Depositor
    David Baker
    Insert
    Top7 K39E K40E V46A K55E
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    K39E K40E V46A K55E
    Available Since
    March 13, 2007
    Availability
    Academic Institutions and Nonprofits only
    12464-plasmid-map-sequence-id-235394

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  29. pX330-Flag-HeFSpCas9-H840A

    Plasmid
    #80456
    Purpose
    high fidelity enhanced SpCas9 mammalian expression vector
    Depositor
    Ervin Welker
    Insert
    HeFSpCas9_H840A
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Promoter
    CBh
    Available Since
    Aug. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80456-plasmid-map-sequence-id-151478

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  30. mP_ILB_Vector 1

    Plasmid
    #232193
    Purpose
    The plasmid vector designed for the expression of four genes in oleaginous yeasts, including Yarrowia lipolytica and Rhodotorula toruloides.
    Depositor
    Matrika Bhattarai
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Yeast
    Available Since
    March 3, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    232193-plasmid-map-sequence-id-463610

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  31. 1203T-CMV-Cas7.1-NLS-bGHpolyA

    Plasmid
    #221540
    Purpose
    Effector only expresses Cas7.1 domain (truncated from DiCas7-11 effector), nuclear localization signal sequence on 5' and 3' end of the coding domain
    Depositor
    Omar Akbari
    Insert
    Truncated DiCas7-11
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    221540-plasmid-map-sequence-id-437823

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  32. pFHMSPN-avi-TEV-LIC V

    Plasmid
    #220885
    Purpose
    Donor vector to generate recombinant baculovirus by site-specific transposition in an E. coli host.
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Baculovirus expresssion
    Tags
    Honeybee melittin signal followed by His, avi and…
    Available Since
    June 20, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    220885-plasmid-map-sequence-id-436698

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  33. p_L_3c_SG_RLuc

    Plasmid
    #208729
    Purpose
    For assessing leaky scanning rates in mammalian cells
    Depositor
    Andrew Firth
    Insert
    ORF3c (ORF3a )
    Tags
    Renilla luciferase
    Expression
    Mammalian
    Available Since
    Jan. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    208729-plasmid-map-sequence-id-420912

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  37. pExp-TFSumo

    Plasmid
    #112576
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and TF (Trigger factor (tig) from E. coli linked to Sumo) fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8xHis-TFSumo and Strep tag
    Expression
    Bacterial
    Available Since
    Feb. 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    112576-plasmid-map-sequence-id-255698

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  38. pExp-Tsf

    Plasmid
    #112578
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and Tsf (elongation factor Ts from E. coli fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8xHis-Tsf and Strep tag
    Expression
    Bacterial
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112578-plasmid-map-sequence-id-217230

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  39. pExp-AFV

    Plasmid
    #112560
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and AFV (Acidianus Filamentous Virus 1 protein) fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8xHis-AFV and Strep tag
    Expression
    Bacterial
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112560-plasmid-map-sequence-id-217216

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  40. NKAP.001.140

    Plasmid
    #137206
    Purpose
    Expresses N-terminal (His)6-TEV cleavage site fusion of human gene or portion of gene in bacterial strains. pET based vector.
    Depositor
    Tara Davis
    Insert
    NKAP (NKAP Human)
    Tags
    (His)6-TEV
    Expression
    Bacterial
    Mutation
    001-140
    Available Since
    Jan. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    137206-plasmid-map-sequence-id-322594

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  41. UC16m

    Plasmid
    #121038
    Purpose
    MoClo golden gate assembly DE part for gQi gRNA (guide RNA for S. pyogenes Cas9; sequence from DOI: 10.1016/j.cell.2013.02.022). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    gQi Cas9 gRNA UC part
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121038-plasmid-map-sequence-id-236383

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  42. UC17m

    Plasmid
    #121039
    Purpose
    MoClo golden gate assembly DE part for gV1 gRNA (guide RNA for S. pyogenes Cas9; sequence from doi: [10.15252/msb.20145735]). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    gV1 Cas9 gRNA UC part
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121039-plasmid-map-sequence-id-236352

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  43. C105m

    Plasmid
    #121008
    Purpose
    MoClo golden gate assembly CD part for sfYFP-AAV (superfolder YFP with AAV degradation tag for ssrA). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    sfYFP-AAV ssrA (has bbsI)
    Use
    Synthetic Biology
    Tags
    AAV degradation tag for ssrA
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121008-plasmid-map-sequence-id-236163

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  44. C106m

    Plasmid
    #121009
    Purpose
    MoClo golden gate assembly CD part for sfYFP-DAS (superfolder YFP with DAS degradation tag for ssrA). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    sfYFP-DAS ssrA (has bbsI)
    Use
    Synthetic Biology
    Tags
    DAS degradation tag for ssrA
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121009-plasmid-map-sequence-id-236172

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  45. C109m

    Plasmid
    #121011
    Purpose
    MoClo golden gate assembly CD part for mfLon-pdt3 (mfLon protease with self-targeting degradation tag pdt3). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    mfLon protease with pdt3 tag
    Use
    Synthetic Biology
    Tags
    pdt3 mfLon degradation tag
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121011-plasmid-map-sequence-id-236323

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  47. DsRed-CPI-17-T38A-NLS

    Plasmid
    #100792
    Purpose
    Mammalian expression of Fluorescent CPI-17 neutral mutation nuclear
    Depositor
    Joseph Gordon
    Insert
    protein phosphatase 1 regulatory inhibitor subunit 14A (PPP1R14A Human)
    Tags
    DsRed
    Expression
    Mammalian
    Mutation
    T38A
    Promoter
    CMV
    Available Since
    Nov. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    100792-plasmid-map-sequence-id-193236

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  48. Py-U5-15kD

    Plasmid
    #25561
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    Py-U5-15kD
    Tags
    His
    Expression
    Bacterial
    Available Since
    May 7, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits

  49. pAAV_BiVIPe4_ChR2_mCherry (AAV PHP.eB)

    Viral Prep
    #213856-PHPeB
    Purpose
    Ready-to-use AAV PHP.eB particles produced from pAAV_BiVIPe4_ChR2_mCherry (#213856). In addition to the viral particles, you will also receive purified pAAV_BiVIPe4_ChR2_mCherry plasmid DNA. Expression of mCherry-tagged channelrhodopsin-2 under the control of the VIP interneuron-targeting enhancer E4. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Gordon Fishell
    Article
    Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1
    Tags
    mCherry
    Available Since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only

  50. pAAV_BiSSTe10_ChR2_mCherry (AAV PHP.eB)

    Viral Prep
    #213815-PHPeB
    Purpose
    Ready-to-use AAV PHP.eB particles produced from pAAV_BiSSTe10_ChR2_mCherry (#213815). In addition to the viral particles, you will also receive purified pAAV_BiSSTe10_ChR2_mCherry plasmid DNA. Expression of mCherry-tagged channelrhodopsin-2 under the control of the SST interneuron-targeting enhancer E10. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Gordon Fishell
    Article
    Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1
    Tags
    mCherry
    Available Since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
Showing: 8651 - 8700 of 15966 results