We narrowed to 20,231 results for: ATO

1. pLCHKOv3-mCherry

   Plasmid

   #209035

   Purpose

   Lentiviral backbone for expressing U6 driven hybrid guide (hg)RNAs with BveI cloning sites, mCherry and puromycin selection marker

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCPH2A.X_S139D-GS3

   Plasmid

   #204748

   Purpose

   Expression of Cas9 and human H2AX with the S139D mutation

   Depositor

   Yuichiro Miyaoka

   Article

   Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

   Insert

   H2AX S139D (H2AX Human)
   

   Expression

   Mammalian

   Mutation

   H2AX with the S139D mutation

   Available Since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. hMLKL NB(1-140)-2xFv-flag

   Plasmid

   #106074

   Purpose

   expression in mammalian cells

   Depositor

   Douglas Green

   Article

   Quarato et al Mol Cell. 2016 Feb 18;61(4):589-601. doi

   Insert

   human MLKL NB(1-140)-2xFv-flag (MLKL Human)
   

   Use

   Retroviral; Tet-on retrovirus

   Tags

   flag

   Expression

   Mammalian

   Mutation

   amino acids 1-140

   Promoter

   Dox-inducible

   Available Since

   March 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCFJ1955

   Plasmid

   #84830

   Purpose

   Minimos transposon with Peft-3:tdTomato:tbb-2 3'UTR and cbr-unc-119 selection. tdTomato was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and synthetic introns

   Depositor

   Andrew Fire

   Article

   Frokjaer-Jensen et al Cell. 2016 Jul 14;166(2):343-57. doi: 10

   Insert

   C. elegans codon optimized tdTomato
   

   Expression

   Bacterial and Worm

   Promoter

   Peft-3

   Available Since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCK301

   Plasmid

   #87767

   Purpose

   E. coli rhaBAD promoter upstream of sfGFP, sfGFP can be replaced with any gene of interest to express using L-rhamnose, three terminators, ampR, pBR322 origin, lacI

   Depositor

   John Heap

   Article

   Kelly et al ACS Synth Biol. 2016 Oct 21;5(10):1136-1

   Insert

   PrhaBAD-sfGFP
   

   Use

   Synthetic Biology

   Tags

   6xHis Tag

   Expression

   Bacterial

   Promoter

   PrhaBAD rhamnose-inducible promoter from E. coli

   Available Since

   May 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pK263.CAG-loxP-stop-loxP-NLSturboRFP-ires-tTA-WPRE

   Plasmid

   #89587

   Purpose

   For nucleus-specific turboRFP labeling in single cells, pK263 should be used with pK031.

   Depositor

   Takuji Iwasato

   Article

   Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

   Insert

   nuclear localization signal (nls) turboRFP
   

   Expression

   Mammalian

   Available Since

   June 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. hMLKL NB(1-140)-2xFv-Venus

   Plasmid

   #106076

   Purpose

   expression in mammalian cells

   Depositor

   Douglas Green

   Article

   Quarato et al Mol Cell. 2016 Feb 18;61(4):589-601. doi

   Insert

   human MLKL NB(1-140)-2xFv-Venus (MLKL Human)
   

   Use

   Retroviral; Tet-on retrovirus

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   amino acids 1-140

   Promoter

   Dox-inducible

   Available Since

   March 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR223-TAF5-FL

   Plasmid

   #209047

   Purpose

   Gateway-compatible entry vector, with insert of the full length TAF5 gene

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   TAF5 full length
   

   Use

   Gateway entry vector

   Expression

   Mammalian

   Available Since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-hSyn-ASAP2s

   Plasmid

   #101276

   Purpose

   Genetically encoded voltage indicator (GEVI) ASAP2s in AAV vector for neuron-specific expression using the promoter hSyn1

   Depositor

   Francois St-Pierre

   Article

   Chamberland et al eLife. 2017 Jul 27;6. pii: e25690. doi:

   Insert

   ASAP2s
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   hSyn (human synapsin)

   Available Since

   Oct. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti.tie1.H2B.eYFP.W

    Plasmid

    #51293

    Purpose

    Lentivector that encodes H2B-eYFP under the transcriptional control of the mouse TIE1 promoter

    Depositor

    Rusty Lansford

    Article

    Sato et al PLoS One. 2010 Sep 14;5(9):e12674. doi:

    Insert

    H2B-eYFP (H2BC21 Human)
    

    Use

    Lentiviral

    Tags

    H2B-eYFP

    Promoter

    mTie1

    Available Since

    June 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. ER-CanlonicSF

    Plasmid

    #178343

    Purpose

    To explore endoplasmic reticulum lactate dynamics

    Depositor

    Alejandro San Martín

    Article

    Aburto et al ACS Sens. 2022 Oct 28. doi: 10.1021/acss

    Insert

    ER-CanlonicSF
    

    Tags

    ER Retention Signal, ER Signal Exon 1, and ER Sig…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLKO.1 MP1 shRNA

    Plasmid

    #26632

    Depositor

    David Sabatini

    Article

    Sancak et al Cell. 2010 Apr 16. 141(2):290-303.

    Insert

    MP1 shRNA (LAMTOR3 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Nov. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

13. PB-CAG-DDdCas9VPP300-T2A-GFP-IRES-Neo

    Plasmid

    #102887

    Purpose

    PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-P300core activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    DDdCas9VP192-P300-T2A-GFP-IRES-Neo
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    CAG

    Available Since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLCHKOv3

    Plasmid

    #209025

    Purpose

    Lentiviral backbone for expressing U6 driven hybrid guide (hg)RNAs with BveI cloning sites and puromycin selection marker

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PB-CAG-DDdCas9VPPH-T2A-GFP-IRES-Neo

    Plasmid

    #102888

    Purpose

    PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-P300core-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    DDdCas9VP192-p65-HSF1-P300-T2A-GFP-IRES-Neo
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    CAG

    Available Since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV_hSyn1_AchLightR

    Plasmid

    #187463

    Purpose

    Expresses red acetylcholine indicator AchLightR in neuronal cells

    Depositor

    Tommaso Patriarchi

    Article

    Kagiampaki et al Nat Methods. 2023 Sep;20(9):1426-1436. d

    Insert

    AchLightR
    

    Use

    AAV

    Tags

    Igκ leader

    Promoter

    human Synapsin-1

    Available Since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. sgRNA1_ASCL1

    Plasmid

    #64129

    Purpose

    Photoactivatable transcription system. Lentiviral expression of ASCL1 sgRNA1. Also contains a CMV-puro-t2A-mCherry expression cassette.

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    sgRNA1_ASCL1 (ASCL1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDisplay-GACh1.0

    Plasmid

    #106072

    Purpose

    express the genetically-encoded fluorescent ACh indicator GACh1.0 in mammalian cells

    Depositor

    Yulong Li

    Article

    Jing et al Nat Biotechnol. 2018 Sep;36(8):726-737.

    Insert

    GPCR activation based ACh sensor (GACh) 1.0
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSLIK-Hygro-Myc-hAgo2

    Plasmid

    #60234

    Purpose

    Express HA-tagged wild-type Ago2 in mammalian cells in a Dox-inducible manner

    Depositor

    Zissimos Mourelatos

    Article

    Liu et al Mol Cell. 2012 May 25;46(4):507-17. doi:

    Insert

    Argonaute2 (AGO2 Human)
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 12, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pLKO.1P POLE1_1

    Plasmid

    #160762

    Purpose

    Suppress POLE1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shPOLE1_1
    

    Use

    Lentiviral

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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