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We narrowed to 63,812 results for: cloning

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Showing: 3461 - 3480 of 63812 results

  10. pET-MCN-10His-mStayGold (E138D)

    Plasmid
    #211361
    Purpose
    Bacterial expression of the bright and photostable monomeric StayGold fluorescent protein (E138D). Codon optimised for expression in Escherichia coli. Contains a N-terminal 10xHis tag.
    Depositor
    Mohan Balasubramanian
    Publication
    Ivorra-Molla et al Nat Biotechnol. 2023 Dec 11. doi: 10.103
    Insert
    mStayGold
    Tags
    10xHis-tag
    Expression
    Bacterial
    Mutation
    E138D
    Promoter
    T7
    Available since
    Dec. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    211361-plasmid-map-sequence-id-417678

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  12. pJR101

    Plasmid
    #187241
    Purpose
    Lentiviral sgRNA vector for Perturb-seq with mU6 sgRNA promoter, CR1 constant region with CS1 capture sequence in stem loop, and UCOE EF1alpha driving PURO-GFP marker expression
    Depositor
    Marco Jost, Jonathan Weissman
    Publication
    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75
    Insert
    Lentiviral sgRNA vector for Perturb-seq with mU6 promoter
    Use
    Lentiviral
    Available since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    187241-plasmid-map-sequence-id-374769

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  14. pTwist-SARS-CoV-2 Δ18 B.1.617.2v1

    Plasmid
    #179905
    Purpose
    Encodes SARS-CoV-2 B.1.617.2v1 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production
    Depositor
    Alejandro Balazs
    Publication
    Garcia-Beltran et al Cell. 2022 Jan 6. pii: S0092-8674(21)014
    Insert
    SARS-CoV-2 B.1.617.2v1 Spike truncated to remove 18 amino acids (S Severe acute respiratory syndrome coronavirus 2)
    Expression
    Mammalian
    Mutation
    T19R; G142D; E156G; Δ157-158; L452R; T478K; D614G…
    Promoter
    CAG
    Available since
    Jan. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    179905-plasmid-map-sequence-id-351283

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  15. AdenoBuilder toolkit

    Plasmid kit
    #1000000176
    Purpose
    Plasmids containing modular inserts that span the human adenovirus serotype 5 (HAdV5) genome that can be assembled in a one-tube reaction.
    Depositor
    Fred Bunz
    Application
    Cloning and Synthetic Biology, Gene Expression and Labeling
    Vector type
    Adenoviral, Mammalian Expression
    Cloning type
    Gibson
    Available since
    Dec. 1, 2021
    Availability
    Academic Institutions and Nonprofits only

  16. Luc-noPS

    Plasmid
    #177940
    Purpose
    Transient mammalian expression of Luc2 (firefly luciferase). Lacks packaging sequence and is used as a negative control transfer plasmid for SARS-CoV-2 virus-like particles.
    Depositor
    Jennifer Doudna
    Publication
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Luc2
    Expression
    Mammalian
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    177940-plasmid-map-sequence-id-348112

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  18. pCMV-ABE8e-NRCH

    Plasmid
    #165416
    Purpose
    Expresses the base editor ABE8e-NRCH in mammalian cells
    Depositor
    David Liu
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ABE8e-NRCH
    Tags
    BP-NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    165416-plasmid-map-sequence-id-334022

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  19. Z-lock cofilin

    Plasmid
    #141137
    Purpose
    controlling the actin-severing ability of cofilin with light
    Depositor
    Klaus Hahn
    Publication
    Stone et al Nat Chem Biol. 2019 Dec;15(12):1183-1190
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Zdk2, Cofilin, LOV2
    Expression
    Mammalian
    Mutation
    mCherry, Zdk2(132F), Cofilin S3A, LOV WT
    Available since
    Dec. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    141137-plasmid-map-sequence-id-319426

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Showing: 3461 - 3480 of 63812 results