We narrowed to 12,557 results for: 109

1. PM18033-PLS-KP4_rpoA-U

   Plasmid

   #190984

   Purpose

   Test the U-to-C editing activity of KP4 on AtrpoA editing site, in human cells

   Depositor

   Bernard Gutmann

   Article

   Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

   Insert

   PLS-KP4
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-PLS-KP5_rpoA-U

   Plasmid

   #190965

   Purpose

   Test the U-to-C editing activity of KP5 on AtrpoA editing site, in E. coli

   Depositor

   Bernard Gutmann

   Article

   Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

   Insert

   PLS-KP5
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. T7-TetO-GFP_ColE1

   Plasmid

   #173193

   Purpose

   Expresses GFP under the control of a T7-TetO promoter. Expression can be repressed via TetR, and repression can be relieved via addition of tetracycline. Contains the ColE1 origin of replication.

   Depositor

   Tara Deans

   Article

   MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

   Insert

   T7-TetO
   

   Use

   Tags

   ssrA degradation tag (DAS+4)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. QUAS-0-T7-TetO-GFP_ColE1

   Plasmid

   #171662

   Purpose

   QUAS directly upstream of a T7 promoter with a TetO operator. Expresses GFP in BL21(DE3) E. coli. Contains the ColE1 origin of replication.

   Depositor

   Tara Deans

   Article

   MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

   Insert

   QUAS-T7-TetO-GFP ssrA-T7 Stop
   

   Use

   Tags

   ssrA degradation tag (DAS+4)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAc-sgRNA-Cas9.scr_1

   Plasmid

   #190703

   Purpose

   Expresses scrambled sgRNA and Cas9-Puro in Drosophila S2 cells

   Depositor

   David Bartel

   Article

   Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

   Insert

   Scrambled sgRNA 1
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Drosophila U6

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAc-sgRNA-Cas9.scr_3

   Plasmid

   #190705

   Purpose

   Expresses scrambled sgRNA and Cas9-Puro in Drosophila S2 cells

   Depositor

   David Bartel

   Article

   Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

   Insert

   Scrambled sgRNA 3
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Drosophila U6

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDF-pntAB

   Plasmid

   #158609

   Purpose

   Low phosphate induction of pntAB

   Depositor

   Michael Lynch

   Article

   Li et al Metab Eng. 2021 Jan 15. pii: S1096-7176(

   Insert

   pntAB(membrane bound proton translocating pyridine nucleotide transhydrogenase)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Insulated ugpBp from E. coli

   Available since

   Jan. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPPC031

   Plasmid

   #171152

   Purpose

   pGNW2 derivative with integration site at P. putida prophage1 for integration of J3-BBa_J23117-mRFP cassette

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

   Insert

   J3-BBa_J23117-mRFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J3-BBa_J23117

   Available since

   Dec. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPPC034

   Plasmid

   #171153

   Purpose

   pGNW2 derivative with integration site at P. putida prophage2 for integration of J3(106)-BBa_J23111-sfGFP cassette

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

   Insert

   J3(106)- BBa_J23111-sfGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J3(106)-BBa_J23111

   Available since

   Dec. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPPC008.106

    Plasmid

    #171141

    Purpose

    Expression of J106 scRNA on pBBR1-GmR plasmid

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

    Insert

    J106 scRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119

    Available since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHR: pmU6 sgChr3q29 2xMS2 pUbC MCP-mCherry-p2a-Puro

    Plasmid

    #174118

    Purpose

    Expression of sgRNA targeting Chr3q29 (chr3: 195478317 - 195506985, hg38)

    Depositor

    Stanley Qi

    Article

    Gao et al Mol Cell. 2021 Aug 13. pii: S1097-2765(2

    Insert

    sgChr3q29 2xMS2 and MCP-mCherry-p2a-Puro
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    mU6 and UbC

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTopo RE2 Donor pLox hPGK-Puro pA pLox

    Plasmid

    #171049

    Purpose

    Donor template for CRISPR/Cas9 targeting of TERT RE2

    Depositor

    Agnel Sfeir

    Article

    Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

    Insert

    Puromycin
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTopo RE3 Donor pLox hPGK-Puro pA pLox

    Plasmid

    #171050

    Purpose

    Donor template for CRISPR/Cas9 targeting of TERT RE3

    Depositor

    Agnel Sfeir

    Article

    Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

    Insert

    Puromycin
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. p663-UBC-miniTurbo-V5-CDKL1_IDG-K

    Plasmid

    #158176

    Purpose

    Gateway destination clone of CDKL1 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-CDKL1 (CDKL1 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. FoxFTVC+bpFOG_MRAS-G22V

    Plasmid

    #107521

    Purpose

    Constitutive activation of FGF/MAPK pathway by M-Ras-G22V mutant

    Depositor

    Lionel Christiaen

    Article

    Razy-Krajka et al Elife. 2018 Feb 6;7. pii: 29656. doi: 10

    Insert

    M-Ras protein
    

    Use

    Tags

    Expression

    Mutation

    changed Glycine 22 to Valine

    Promoter

    TVC-specific FoxF enhancer (FoxFTVC+bpFOG)

    Available since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX-4T1 mouse Tankyrase1 ARC2-3 (308-655)

    Plasmid

    #132637

    Purpose

    expresses mouse Tankyrase1 ARC2-3 (308-655) in E. coli

    Depositor

    Wenqing Xu

    Article

    Morrone et al Proc Natl Acad Sci U S A. 2012 Jan 31;10

    Insert

    Tankyrase1 (308-655)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSTAP-seq_fly_spike-in

    Plasmid

    #125151

    Purpose

    Vector to measure CP candidates (spike-in) expression by determining the abundance of transcripts originating from the candidate by a D.pse enhancer in fly cells

    Depositor

    Alexander Stark

    Article

    Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:

    Insert

    D. pseudoobscura peak #47 enhancer
    

    Use

    Stap-seq screening vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PTE_25

    Plasmid

    #111638

    Purpose

    Funclib design PTE_25

    Depositor

    Sarel Fleishman

    Article

    Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

    Insert

    PTE_25
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    pTac

    Available since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PTE_26

    Plasmid

    #111639

    Purpose

    Funclib design PTE_26

    Depositor

    Sarel Fleishman

    Article

    Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

    Insert

    PTE_26
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    pTac

    Available since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PTE_27

    Plasmid

    #111640

    Purpose

    Funclib design PTE_27

    Depositor

    Sarel Fleishman

    Article

    Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

    Insert

    PTE_27
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    pTac

    Available since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge