We narrowed to 7,237 results for: CIT

1. pGG431

   Plasmid

   #165615

   Purpose

   Vector for expression of SpCas9 in yeast after assembly with PID fragments: TEF1p-SpCas9::KanR-1xNLS-CYC1t (KanR cassette in place of the PID)

   Depositor

   Marcus Noyes

   Article

   Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

   Insert

   TEF1p-SpCas9::KanR(in place of PID)-1xNLS-CYC1t (S. cerevisiae TEF promoter driving SpCas9 with KanR cassette replacing PID)
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Yeast

   Mutation

   Corrected homology relative to wild type SpCas9 (…

   Promoter

   TEF1

   Available since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDestTol2-actinb-kcnj1b-IRES-EGFP

   Plasmid

   #164946

   Purpose

   Tol2 construct: actinb (actb2) promoter driven zebrafish kcnj1b and EGFP

   Depositor

   GuangJun Zhang

   Article

   Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

   Insert

   kcnj1b (kcnj1b Zebrafish)
   

   Use

   Tol2 transposon destination vector

   Tags

   IRES-EGFP

   Expression

   Mutation

   Promoter

   actinb (actb2) promoter

   Available since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDGB3 alpha2 P35S:MS2-VP64:Tnos (GB1395)

   Plasmid

   #160609

   Purpose

   TU for the constitutive expresion of the viral coat protein MS2 fused on C-terminal with the VP64

   Depositor

   Diego Orzaez

   Article

   Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

   Insert

   P35S:MS2-VP64:Tnos
   

   Use

   Tags

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   P35S

   Available since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. hKCNB1 L211P

   Plasmid

   #124487

   Purpose

   missense mutation in children with EOEE. HA tagged in C-terminus, pCIneo vector

   Depositor

   Federico Sesti

   Article

   Yu et al FASEB J. 2019 Dec;33(12):14680-14689. do

   Insert

   human KCNB1 (KCNB1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   changed leucine 211 to proline

   Promoter

   Available since

   Sept. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. hKCNB1 R312H

   Plasmid

   #124488

   Purpose

   missense mutation in children with EOEE. HA tagged in C-terminus, pCIneo vector

   Depositor

   Federico Sesti

   Article

   Yu et al FASEB J. 2019 Dec;33(12):14680-14689. do

   Insert

   human KCNB1 (KCNB1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   changed arginine 312 to histidine

   Promoter

   Available since

   Sept. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. hKCNB1 G381R

   Plasmid

   #124490

   Purpose

   missense mutation in children with EOEE. HA tagged in C-terminus, pCIneo vector

   Depositor

   Federico Sesti

   Article

   Yu et al FASEB J. 2019 Dec;33(12):14680-14689. do

   Insert

   human KCNB1 (KCNB1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   changed glycine at 381 to arginine

   Promoter

   Available since

   Sept. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. hKCNB1 F416L

   Plasmid

   #124491

   Purpose

   missense mutation in children with EOEE. HA tagged in C-terminus, pCIneo vector

   Depositor

   Federico Sesti

   Article

   Yu et al FASEB J. 2019 Dec;33(12):14680-14689. do

   Insert

   human KCNB1 (KCNB1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   changed phenyalanine 416 to leucine

   Promoter

   Available since

   Sept. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. TRAV29

   Plasmid

   #123401

   Purpose

   construction of TCR

   Depositor

   Catherine Wu

   Article

   Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

   Insert

   TRAV29 (TRAV29DV5 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   Available since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. hKCNB1 G379R

   Plasmid

   #124489

   Purpose

   missense mutation in children with EOEE. HA tagged in C-terminus, pCIneo vector

   Depositor

   Federico Sesti

   Article

   Yu et al FASEB J. 2019 Dec;33(12):14680-14689. do

   Insert

   human KCNB1 (KCNB1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   changed glycine at 379 to arginine

   Promoter

   Available since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti C-MYC-DDK Puro PNRC1-W300A

    Plasmid

    #123301

    Purpose

    Mammalian expression of PNRC1-W300A mutant C-MYC-FLAG

    Depositor

    Giovanni Tonon

    Article

    Gaviraghi et al EMBO J. 2018 Dec 3;37(23). pii: embj.201

    Insert

    PNRC1 (PNRC1 Human)
    

    Use

    Lentiviral

    Tags

    MYC-FLAG

    Expression

    Mammalian

    Mutation

    W300A

    Promoter

    Available since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. DLG3_PDZ_2

    Plasmid

    #103947

    Purpose

    Protein expression and purification of NE-DLG PDZ2 domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    DLG3 (DLG3 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. MAGI1_PDZ_1

    Plasmid

    #103898

    Purpose

    Protein expression and purification of MAGI1 PDZ1 domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    MAGI1 (MAGI1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. MAGI1_PDZ_4

    Plasmid

    #103901

    Purpose

    Protein expression and purification of MAGI1 PDZ4 domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    MAGI1 (MAGI1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTE3314

    Plasmid

    #107530

    Purpose

    Expresses human codon optimized FnCpf1(RVR mutant) in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Insert

    hFnCpf1(RVR)
    

    Use

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    N607R, K613V, N617R

    Promoter

    CMV

    Available since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTE3319

    Plasmid

    #107533

    Purpose

    Expresses human codon optimized MbCpf1(RR mutant) in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Insert

    hMbCpf1(RR)
    

    Use

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    N576R, K637R

    Promoter

    CMV

    Available since

    April 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTE3316

    Plasmid

    #107532

    Purpose

    Expresses human codon optimized FnCpf1(RR mutant) in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Insert

    hFnCpf1(RR)
    

    Use

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    N607R, K671R

    Promoter

    CMV

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTE3317

    Plasmid

    #107531

    Purpose

    Expresses human codon optimized MbCpf1(RVR mutant) in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Insert

    hMbCpf1(RVR)
    

    Use

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    N576R, K582V, N586R

    Promoter

    CMV

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTE3179

    Plasmid

    #107528

    Purpose

    Expresses Mb crRNA and mCherry in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Inserts

    Mb crRNA
    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and human U6

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHH0103_FRK-1/1

    Plasmid

    #91549

    Purpose

    Protein expression and purification of human SH3 domain construct FRK-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    FRK-1/1 (FRK Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHH0103_ITSN1-1/5-IS3/3

    Plasmid

    #91560

    Purpose

    Protein expression and purification of human SH3 domain construct ITSN1-1/5-IS3/3

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    ITSN1-1/5-IS3/3 (ITSN1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge