We narrowed to 6,241 results for: nls

1. pCEE

   Plasmid

   #159746

   Purpose

   Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by CFP fluoresce.

   Depositor

   Wolfgang Lukowitz

   Article

   Angulo et al bioRxiv

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Oct. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. TRE-dCas9(sa)-VP64-AAV

   Plasmid

   #213035

   Purpose

   dCas9-VP64 expressed under control of TRE

   Depositor

   Nadav Ahituv

   Article

   Nguyen et al Nat Biotechnol. 2025 Feb 4:10.1038/s4158

   Insert

   dCas9
   

   Use

   AAV and CRISPR

   Tags

   NLS and NLS-VP64

   Promoter

   CMV

   Available Since

   Oct. 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. TOPO-EXOC3 (-1557 to-1046)

   Plasmid

   #226446

   Purpose

   For subcloning of human EXOC3 promoter (base pairs -1557 to-1046) or for assays using M13 phage

   Depositor

   Glen Borchert

   Article

   DeMeis et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

   Insert

   EXOC3 (-1557 to-1046) (EXOC3 Human)
   

   Use

   Cloning vector

   Mutation

   No

   Available Since

   Sept. 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. TOPO-EXOC3 (-2455 to -1951)

   Plasmid

   #226441

   Purpose

   For subcloning of human EXOC3 promoter (base pairs -2455 to -1951) or for assays using M13 phage

   Depositor

   Glen Borchert

   Article

   DeMeis et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

   Insert

   EXOC3 (-2455 to -1951) (EXOC3 Human)
   

   Use

   Cloning vector

   Mutation

   No

   Available Since

   July 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. TOPO-EXOC3 (-1592 to -1046)

   Plasmid

   #226444

   Purpose

   For subcloning of human EXOC3 promoter (base pairs -1592 to -1046) or for assays using M13 phage

   Depositor

   Glen Borchert

   Article

   DeMeis et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

   Insert

   EXOC3 (-1592 to -1046) (EXOC3 Human)
   

   Use

   Cloning vector

   Mutation

   No

   Available Since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. TOPO-EXOC3 (-1591 to -1009)

   Plasmid

   #226443

   Purpose

   For subcloning of human EXOC3 promoter (base pairs -1591 to -1009) or for assays using M13 phage

   Depositor

   Glen Borchert

   Article

   DeMeis et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

   Insert

   EXOC3 (-1591 to -1009) (EXOC3 Human)
   

   Use

   Cloning vector

   Mutation

   No

   Available Since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. TOPO-EXOC3 (-1701 to -1594)

   Plasmid

   #226442

   Purpose

   For subcloning of human EXOC3 promoter (base pairs -1701 to -1594) or for assays using M13 phage

   Depositor

   Glen Borchert

   Article

   DeMeis et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

   Insert

   EXOC3 (-1701 to -1594) (EXOC3 Human)
   

   Use

   Cloning vector

   Mutation

   No

   Available Since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. TOPO-EXOC3 (-1592 to -1444)

   Plasmid

   #226445

   Purpose

   For subcloning of human EXOC3 promoter (base pairs -1592 to -1444) or for assays using M13 phage

   Depositor

   Glen Borchert

   Article

   DeMeis et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

   Insert

   EXOC3 (-1592 to -1444) (EXOC3 Human)
   

   Use

   Cloning vector

   Mutation

   No

   Available Since

   July 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. TOPO-EXOC3 (-1557 to-1444)

   Plasmid

   #226447

   Purpose

   For subcloning of human EXOC3 promoter (base pairs -1557 to-1444) or for assays using M13 phage

   Depositor

   Glen Borchert

   Article

   DeMeis et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

   Insert

   EXOC3 (-1557 to-1444) (EXOC3 Human)
   

   Use

   Cloning vector

   Mutation

   No

   Available Since

   July 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-SpCas9-P2A-EGFP (MSP2582)

    Plasmid

    #223067

    Purpose

    pCAG human codon optimized wild-type SpCas9 expression plasmid with C-terminal NLS(SV40)-3xFLAG-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized SpCas9-P2A-EGFP
    

    Use

    CRISPR

    Tags

    NLS(SV40)-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-CMV-FLEX-SaCas9-U6-sgKcnma1

    Plasmid

    #209199

    Purpose

    Mutagenesis of Kcnma1

    Depositor

    Larry Zweifel

    Article

    Juarez et al Sci Adv. 2023 Aug 11;9(32):eadg8869. doi

    Insert

    Kcnma1 (Kcnma1 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Promoter

    CMV

    Available Since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. Ubiquiton Yeast Kit

    Plasmid Kit

    #1000000243

    Purpose

    The Ubiquiton system allows the inducible, linkage-specific polyubiquitylation of a protein of interest, accommodating for linear (M1-), K48-, and K63-linkage.

    Depositor

    Helle Ulrich

    Available Since

    May 31, 2024

    Availability

    Academic Institutions and Nonprofits only

13. pPGK-GZF1-RR

    Plasmid

    #35092

    Depositor

    David Segal

    Article

    Bhakta et al Methods Mol Biol. 2010;649:3-30.

    Insert

    ZFN GZF1-Fok(RR)
    

    Tags

    HA and SV40 NLS

    Expression

    Mammalian

    Mutation

    contains the "RR" obligate heterodimer …

    Promoter

    PGK

    Available Since

    May 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPGK-GZF3-DD

    Plasmid

    #35093

    Depositor

    David Segal

    Article

    Bhakta et al Methods Mol Biol. 2010;649:3-30.

    Insert

    ZFN GZF3-Fok(DD)
    

    Tags

    HA and SV40 NLS

    Expression

    Mammalian

    Mutation

    contains the "DD" obligate heterodimer …

    Promoter

    PGK

    Available Since

    May 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-ABE-P48R-UGI

    Plasmid

    #161817

    Purpose

    Expresses ABE-P48R-UGI in mammalian cells

    Depositor

    Sangsu Bae

    Insert

    bpNLS-TadA7.10(P48R)-32AA linker-hSpCas9n(D10A)-10AA linker-UGI-10AA linker-UGI-bpNLS-P2A-EGFP-NLS
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    D10A in S. pyogenes Cas9, TadA mutations describe…

    Available Since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPuroG1

    Plasmid

    #200224

    Purpose

    puroR nls-mNG marked rapid RMCE integration vector

    Depositor

    Michael Nonet

    Article

    Nonet et al Genetics. 2023 Apr 20:iyad072. doi: 10.1

    Type

    Empty backbone

    Expression

    Worm

    Available Since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. APEX2 VLP

    Plasmid

    #182211

    Purpose

    Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and APEX2 tagged with VP2C (GAL10 promoter).

    Depositor

    Claudia Vickers

    Inserts

    VP2C-APEX2
    Murine polyomavirus deltaVP1 (NLS deletion mutant)
    

    Use

    Synthetic Biology; Tem imaging

    Expression

    Yeast

    Promoter

    GAL1 and GAL10

    Available Since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKG210

    Plasmid

    #69761

    Purpose

    Retroviral expression of CENP-T aa 1-375

    Depositor

    Iain Cheeseman

    Insert

    CENP-T aa 1-375 (CENPT Human)
    

    Use

    Retroviral

    Tags

    LacI-NLS and mCherry

    Expression

    Mammalian

    Mutation

    e.coli optimized sequence

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pKG212

    Plasmid

    #69758

    Purpose

    Retroviral expression of CENP-T aa 1-242

    Depositor

    Iain Cheeseman

    Insert

    CENP-T aa 1-242 (CENPT Human)
    

    Use

    Retroviral

    Tags

    LacI-NLS and mCherry

    Expression

    Mammalian

    Mutation

    e.coli optimized sequence

    Available Since

    Nov. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

20. PX391 Sphaerochaeta globus str. Buddy Cas9

    Plasmid

    #68328

    Purpose

    Sphaerochaeta globus str. Buddy Cas9

    Depositor

    Feng Zhang

    Insert

    SgCas9
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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